We narrowed to 17,810 results for: Met;

1. mIFP12-CD81-10

   Plasmid

   #56248

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 683, Emission: 704

   Depositor

   Michael Davidson

   Article

   Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

   Insert

   CD81 (CD81 Human)
   

   Use

   Tags

   mIFP12

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192789

   Purpose

   aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody minimizer
   

   Use

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMOS010: pLX304-CheRiff-GFP

   Plasmid

   #62986

   Purpose

   Lentivirus production of CheRiff channelrhodopsin actuator

   Depositor

   Adam Cohen

   Article

   Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

   Insert

   CheRiff-eGFP
   

   Use

   Lentiviral; Gateway destination vector

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-VTKS6

   Plasmid

   #170857

   Purpose

   AAV backbone for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of any transgene in neurons under the control of the hSyn1 promoter.

   Depositor

   Jordane Dimidschstein

   Article

   Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1

   Plasmid

   #218524

   Purpose

   sgRNA targeting human KIAA0141/DELE1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   DELE1 (DELE1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2

   Plasmid

   #218525

   Purpose

   sgRNA targeting human KIAA0141/DELE1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   DELE1 (DELE1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY036_ATP1A1_G5_Array

   Plasmid

   #86620

   Purpose

   Vector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G5 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmid

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 G5 crRNA + user-specified crRNA + AsCpf1-3xHA
   

   Use

   CRISPR; Co-selection via hdr using ouabain

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ2842 pPB: CAG-GID1-VPR-IRES-Zeo-WPRE PGK-GAI-tagBFP-SadCas9

   Plasmid

   #84244

   Purpose

   Expresses GA-inducible VPR-Sa dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   GAI-tagBFP-Sa dCas9
   GID1-VPR
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   GAI-tagBFP-Nucleoplasmin NLS, GID1, IRES-Zeocin, …

   Expression

   Mammalian

   Mutation

   D10A, N580A and Synonymous mutation in PYL1 to re…

   Promoter

   CAG and PGK

   Available since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-STIM1-NN_puromycin

   Plasmid

   #65310

   Purpose

   Luminal ER hook only (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   STIM1 mutated for binding motif to microtubule (STIM1 Human)
   

   Use

   Tags

   streptavidin

   Expression

   Mammalian

   Mutation

   2 amino acids of STIM1 (IP from SxIP motif) were …

   Promoter

   CMV

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-VTKS5-GFP

    Plasmid

    #178713

    Purpose

    AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

    Depositor

    Jordane Dimidschstein

    Article

    Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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