We narrowed to 9,182 results for: Pol

1. pLKO.1-TRC.mKO2

   Plasmid

   #85208

   Purpose

   Backbone for lentiviral gene silencing with monomeric Kusabira-Orange2 expression

   Depositor

   Sandra Martha Gomes Dias

   Article

   Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   RNA polymerase III promoter for human U6 snRNA fo…

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBacM13-8His-ZZ-LTLT-Hook3-SNAPf

   Plasmid

   #130976

   Purpose

   Expression of 8His-ZZ-LTLT tagged Hook3-SNAPf in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   Hook3-SNAPf (HOOK3 Human)
   

   Tags

   8His-ZZ-LTLT and SNAPf

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET30-ARH3-His-Sumo-HA

   Plasmid

   #111578

   Purpose

   Expresses ARH3 (amino acids 14-363) in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   ARH3 (ADPRS Human)
   

   Tags

   6xHis-Sumo and HA

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-13

   Promoter

   T7

   Available Since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFl Strep-sumo-TEV-hAgo2

   Plasmid

   #136235

   Purpose

   Expresses human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Tags

   Strep Sumo

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAGM22082_cRed

   Plasmid

   #117225

   Purpose

   pET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.

   Depositor

   Sylvestre Marillonnet

   Article

   Pullmann et al Sci Rep. 2019 Jul 29;9(1):10932. doi: 10

   Insert

   Canthaxanthin Operon
   

   Tags

   34 amino acid N-terminal linker, Hexahistidine Ta…

   Expression

   Bacterial

   Mutation

   removed Type IIS restriction sites within the bac…

   Promoter

   T7 RNA Polymerase

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB PUb-integrase

   Plasmid

   #183966

   Purpose

   piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   PhiC31 integrase
   

   Use

   Piggybac insect transgenesis destination vector

   Promoter

   Aedes aegypti Polyubiquitin

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. M2e-noro-VLP

   Plasmid

   #201192

   Purpose

   Expresses noro-VLP decorated with influenza M2e in insect cells

   Depositor

   Vesa Hytönen

   Article

   Lampinen et al Front Cell Infect Microbiol. 2023 Jun 22

   Insert

   M2e-noro-VLP
   

   Tags

   Influenza M2e peptide

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-DIO-NS1

   Plasmid

   #175273

   Purpose

   AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. p59sheLL

   Plasmid

   #40628

   Depositor

   Simon Beddows

   Article

   Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

   Inserts

   HPV59 L1
   HPV59 L2
   

   Expression

   Mammalian

   Mutation

   Codon optimized for mammalian expression

   Promoter

   CMV and Polio IRES

   Available Since

   Oct. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. 498 pSG5L HA RB del663 (NAAIRS)

    Plasmid

    #10728

    Depositor

    William Sellers

    Article

    Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

    Insert

    RB del663 (RB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 664 to 669 were replaced by NAAIRS, a fl…

    Available Since

    Sept. 27, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-hPPP3CC-FLAG

    Plasmid

    #179162

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. p39sheLL

    Plasmid

    #40627

    Depositor

    Simon Beddows

    Article

    Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

    Inserts

    HPV39 L1
    HPV39 L2
    

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression

    Promoter

    CMV and Polio IRES

    Available Since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pAcUW51-gEgIL

    Plasmid

    #11622

    Depositor

    Pamela Bjorkman

    Article

    Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

    Insert

    HSV-1 glycoprotein E and HSV-1 glycoprotein I
    

    Tags

    His

    Expression

    Insect

    Mutation

    Dicistronic vector containing HSV-1 gE (KOS strai…

    Available Since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pCAG-mPpp3cc-FLAG-6XHIS

    Plasmid

    #179160

    Purpose

    Expression vector of mouse protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc) tagged with FLAG and 6X HIS at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

    Insert

    protein phosphatase 3, catalytic subunit, gamma isoform (Ppp3cc Mouse)
    

    Tags

    6XHIS and FLAG

    Expression

    Mammalian

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Fireworks RFP[PTC+] (AVA2626)

    Plasmid

    #85444

    Purpose

    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-HygromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCsy_complex

    Plasmid

    #89232

    Purpose

    Plasmid contains the genes Csy1, Csy2, Csy3, Csy4, cloned as a polycistronic operon from bacterium Pseudomonas aeruginosa PA14. Csy3 includes an N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Inserts

    Csy1
    Csy2
    Csy3
    Csy4
    

    Tags

    6xHis-TEV protease

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. Fireworks GFP[PTC+] (AVA2600)

    Plasmid

    #85446

    Purpose

    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a PTC-containing beta-globin reporter.

    Depositor

    Joan Steitz

    Article

    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:

    Insert

    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)(PTC39)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    

    Use

    Minimal backbone fragment for low-copy bacterial …

    Expression

    Mammalian

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB’ CbbL Y72R

    Plasmid

    #162707

    Purpose

    Carboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomes

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Expression

    Bacterial

    Mutation

    CbbL Y72R; Substitution of CsoS2-binding tyrosine…

    Promoter

    PLtet0-1 promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-PTBP1-RRM34

    Plasmid

    #89156

    Purpose

    Recombinant expression of PTBP1-RRM34 in E.coli

    Depositor

    Frédéric Allain

    Article

    Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

    Insert

    Polypyrimidine Tract Binding Protein 1 RNA Recognition Motif 3+4 (PTBP1 Human)
    

    Tags

    hexa His

    Expression

    Bacterial

    Mutation

    contains RNA Recognition Motif 3+4

    Promoter

    T7

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMJA219= pcDNA3.1V5-completeTRABV-CMVp

    Plasmid

    #116878

    Purpose

    mammalian expression vector containing TCR sequences

    Depositor

    Marcos Alcocer

    Article

    Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

    Insert

    complete TRAV10-TRBV25-CMVp rabbitpolyA (TRAV10, TRBV25-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV Promoter

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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