We narrowed to 63,772 results for: cell

1. pcDNA5/FRT/TO_CalR_STf_KDEL

   Plasmid

   #182004

   Purpose

   CMV overexpression of SNAP-tag fast in the endoplasmic reticulum of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   CalR_STf_KDEL
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5/FRT/TO_b4g-STf

   Plasmid

   #182005

   Purpose

   CMV overexpression of SNAP-tag fast in the golgi apparatus of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   b4g-STf
   

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc-sgRNA-Cas9.scr_1

   Plasmid

   #190703

   Purpose

   Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Scrambled sgRNA 1
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   Drosophila U6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAc-sgRNA-Cas9.scr_3

   Plasmid

   #190705

   Purpose

   Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

   Depositor

   David Bartel

   Article

   Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

   Insert

   Scrambled sgRNA 3
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   Drosophila U6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. YIplac211-iGFP-DRS2

   Plasmid

   #177104

   Purpose

   Expresses iGFP-Drs2 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   DRS2 (DRS2 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-PGK-SNAP-MD-OCT4

   Plasmid

   #115691

   Purpose

   Constitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a mitotic degron (MD)

   Depositor

   David Suter

   Article

   Friman et al eLife. 2019 Dec 3;8. pii: 50087. doi: 10

   Insert

   SNAP-MD-OCT4
   

   Use

   Lentiviral

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIplac211-NHX1-iGFP

   Plasmid

   #177108

   Purpose

   Expresses Nhx1-iGFP in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   NHX1 (NHX1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-TREX1 g3 Cas9-T2A-mCherry

   Plasmid

   #164252

   Purpose

   Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

   Depositor

   John Maciejowski

   Article

   Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

   Insert

   sgTREX1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC8

   Plasmid

   #170757

   Purpose

   Co-express in mammalian cells two target proteins C-terminally fused to V5 and FLAG tags respectively, using an internal ribosome entry site and two multi-cloning sites.

   Depositor

   Roberto Dominguez

   Article

   Carman et al J Biol Chem. 2021 Oct;297(4):101154. doi

   Type

   Empty backbone

   Tags

   site 1- V5 tag and site 2- FLAG tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Rab37

    Plasmid

    #140886

    Purpose

    Expression of zebrafish GFP-tagged Rab37 in mammalian cells.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Rab37
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAV0517

    Plasmid

    #133492

    Purpose

    Expression of nucleo-cytoplasmic GFP in P-cells

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pHis5(StuI)-p(map3)-GFP-terminator(nmt)
    

    Expression

    Yeast

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV0763

    Plasmid

    #133497

    Purpose

    Expression of nucleo-cytoplasmic mTagBFP2 in M-cells

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(mam1*)-mTagBFP2-terminator(ScADH1)-kanMX
    

    Expression

    Yeast

    Available Since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. IgH P+ VA20

    Plasmid

    #127249

    Purpose

    IgH V-region

    Depositor

    Christine Milcarek

    Article

    Shell et al J Immunol. 2007 Dec 1;179(11):7663-73.

    Insert

    Promoter+V-region of IgH of A20 B cell line
    

    Use

    Other

    Promoter

    IgH

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. IgH intron enhancer

    Plasmid

    #127247

    Purpose

    To maximize gene expression in B cells

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    heavy chain enhancer
    

    Expression

    Mammalian

    Available Since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZF-AA-GCN

    Plasmid

    #127479

    Purpose

    coding sequence for synthetic zinc finger ZF-AA-GCN

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-AA fused with GCN4 leucine zipper
    

    Use

    Cell-free transcription–translation

    Tags

    GCN4 leucine zipper

    Promoter

    T7

    Available Since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

    Plasmid

    #127409

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, E309C …

    Promoter

    CMV

    Available Since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-MBP1-K295TAG-W340A-S233H-T237C-CAAX.pcDNA3-k

    Plasmid

    #127403

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available Since

    July 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FLAG-MBP1-E322TAG-K305H-E309H-W340A-CAAX.pcDNA3-k

    Plasmid

    #127407

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Tags

    CAAX and FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, K305H,…

    Promoter

    CMV

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FLAG-MBP1-E322TAG-K305H-E309H-W340A.pcDNA3-k

    Plasmid

    #127406

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: E322 to Amber codon, K305H,…

    Promoter

    CMV

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FLAG-MBP1-K295TAG-W340A-S233H-T237C.pcDNA3-k

    Plasmid

    #127402

    Purpose

    Expresses MBP in mammalian cells. Amber codon at AA# 295 as a noncanonical amino acid incorporation site

    Depositor

    Sharona Gordon

    Article

    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1

    Insert

    Maltose Binding Protein
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Amino acid mutations: K295 to Amber codon, W340A,…

    Promoter

    CMV

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge