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p2703-AGER-gRNA2

Plasmid

#216472

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2704-AGER-gRNA3

Plasmid

#216473

Purpose

to express Cas9 from S. pyogenes with 2A-eGFP and sgRNA targeting human AGER endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

sgRNA targeting human AGER locus

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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INS-3'gRNA

Plasmid

#210468

Purpose

gRNA targeting INS 3' terminal for CRISPR-Cas9-mediated knock-in

Depositor

Kai Wang

Article

Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

Insert

INS (INS Human)

Use

CRISPR

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMeCP2Y pc644

Plasmid

#167565

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Insert

Methyl-CpG binding protein 2 (Mecp2 Rat)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE P1214R

Plasmid

#166810

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. LL1193EE and P1214R mutations abolish DPP9 association.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

LL1193EE, P1214R

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG LL1193EE

Plasmid

#166805

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The LL1193E mutant abolishes FL-NLRP1 association with DPP9 at interface I.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

LL193EE

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG K1277E

Plasmid

#166808

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The K1277E mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K1277E

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG H1276G

Plasmid

#166807

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The H1276G mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

H1276G

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 LIC 6A NLRP1-FLAG P1214R

Plasmid

#166806

Purpose

Mammalian cell expression vector for FLAG-tagged NLRP1. The P1214R patient mutant abolishes NLRP1-CT association with DPP9 at interface II.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

P1214R

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pB6-Lrig1-T2A-sfGFP-iCre-ERT2-FNF-TK

Plasmid

#126651

Purpose

B6J Lrig1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

Lrig1 (Lrig1 Mouse)

Use

Cre/Lox and Mouse Targeting

Mutation

C-terminal fusion of T2A-sfGFP-iCre-ERT2

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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