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TRIM33 gRNA (BRDN0001162291)

Plasmid

#77097

Purpose

3rd generation lentiviral gRNA plasmid targeting human TRIM33

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

TRIM33 (TRIM33 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ACVR1 gRNA (BRDN0001145448)

Plasmid

#76757

Purpose

3rd generation lentiviral gRNA plasmid targeting human ACVR1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ACVR1 (ACVR1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CHEK2 gRNA (BRDN0001146385)

Plasmid

#76488

Purpose

3rd generation lentiviral gRNA plasmid targeting human CHEK2

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CHEK2 (CHEK2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKCB gRNA (BRDN0001148507)

Plasmid

#75954

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKCB

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKCB (PRKCB Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ALDH18A1 gRNA (BRDN0001487061)

Plasmid

#77996

Purpose

3rd generation lentiviral gRNA plasmid targeting human ALDH18A1

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ALDH18A1 (ALDH18A1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 6 serines mutated to alanines

Plasmid

#69815

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Publication

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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hPlekhm1_pIRES puro Glue

Plasmid

#73453

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Publication

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Plekhm1 (PLEKHM1 Human)

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS2

Plasmid

#59390

Purpose

Destination vector with RBPMS2 for stable cell line generation

Depositor

Thomas Tuschl

Publication

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RBPMS2 (RBPMS2 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRS426-TUB1-internal6xHis (1-440)

Plasmid

#60391

Purpose

Expression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoter

Depositor

Ron Vale

Publication

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Mutation

internal 6xHis (see supplement figure 4), amino a…

Promoter

GAL

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pDEST myc MOV10 K530A

Plasmid

#51719

Purpose

plasmid expresses myc-tagged MOV10 K530A

Depositor

Markus Landthaler

Publication

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MOV10 K530A (MOV10 Human)

Tags

myc

Expression

Mammalian

Mutation

K530A

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

Plasmid

#32735

Depositor

Frank McCormick

Publication

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 Promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

Promoter

-962 CCND1

Available since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
Y pFe-YcLoxP-YnAtt

Plasmid

#24510

Depositor

Jehonathan Pinthus

Publication

Lu et al Nat Prec (2008).

Insert

Yn, Yc, ECFP, EYFP

Tags

ECFP and EYFP

Expression

Mammalian

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only

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L1-1176

Plasmid

#13264

Depositor

Vance Lemmon

Publication

Cheng et al J Neurosci. 2005 Jan 12. 25(2):395-403.

Insert

L1CAM (L1CAM Human)

Expression

Bacterial

Mutation

Truncated before RSLE region

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pG20_TEV_2xStrepII_FG

Plasmid

#240675

Purpose

Expression plasmid for TEV-2xStrepII-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Publication

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-2xStrepII

Expression

Plant

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pGL0_22 [pSG5 ORI]

Plasmid

#198935

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI

Use

Synthetic Biology

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_21 [UARS1 ORI]

Plasmid

#198934

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

UARS1 ORI

Use

Synthetic Biology

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_19 [panARS ORI]

Plasmid

#198932

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

panARS ORI

Use

Synthetic Biology

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_11 [pWKS1 ORI]

Plasmid

#198925

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pWKS1 ORI

Use

Synthetic Biology

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-xylA

Plasmid

#89964

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting xylA.

Depositor

Michael Lynch

Publication

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

xylA gRNA

Expression

Bacterial

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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CtkA_full_pET21a

Plasmid

#27428

Depositor

SeWon Suh

Publication

Kim et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Cell translocating kinas A (jhp0940 H. pylori)

Tags

His

Expression

Bacterial

Available since

May 2, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article