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pCOLA kiwi sensor sfGFP

Plasmid

#107368

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

kiwi-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-T43NTR-TrxA-H10

Plasmid

#121677

Purpose

Bacterial expression of neurotensin receptor fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60

Insert

neurotensin receptor (Ntsr1 Rat)

Tags

TrxA-H10

Expression

Bacterial

Mutation

deleted amino acids 1-42

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
mEmerald-MCM3-N-22

Plasmid

#54166

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM3

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pMAL498

Plasmid

#169154

Purpose

Encodes OptoQ-INVRT4

Depositor

Jose Avalos

Article

Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

Plasmid

#199347

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-3C

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA iRFP713_zhang2.0

Plasmid

#167916

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

Plasmid

#172225

Purpose

Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

Depositor

Alexander Meissner

Article

Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

Insert

sgRNA

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I gamma

Plasmid

#22156

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Available Since

Dec. 9, 2009

Availability

Academic Institutions and Nonprofits only
GFP/WT SP M85

Plasmid

#89317

Purpose

Expression of mouse GFP/WT spastin M85 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2008 Feb 27;28(9):2147-57. d

Insert

SPAST M85 (Spast Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pKF257

Plasmid

#52533

Purpose

expresses I-SceI nuclease in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

I-SceI (SCEI Budding Yeast)

Tags

HA and NLS

Expression

Insect

Promoter

tubulin

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pEBB-II-I-GFP gamma isoform

Plasmid

#22148

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pEBB-II-I-GFP alpha isoform

Plasmid

#22146

Depositor

Ananda Roy

Article

Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

Insert

TFII-I (GTF2I Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pME18S-3HA-mULK1(K46N)

Plasmid

#22897

Depositor

Masaaki Muramatsu

Article

Yan et al Biochem Biophys Res Commun. 1998 May 8.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Tags

3xHA

Expression

Mammalian

Mutation

changed Lysine 46 to Aspargine

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only
H2A-mTurquoise-RHOG-QL-deltaCAAX

Plasmid

#176096

Purpose

Constitutively active, nuclear localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Expression

Mammalian

Mutation

QL

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p6656 MSCV-P C-Flag HA 25E7-Kozak

Plasmid

#35149

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV25 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 9, 2012

Availability

Academic Institutions and Nonprofits only
pET29b-mVenus-dspB(E184Q)-6xHis

Plasmid

#176576

Purpose

Plasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al Methods Enzymol. 2022;665:209-231. doi:

Insert

Dispersin B

Tags

Hexahistidine tag and mVenus

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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Munc18cwt-pDEST27

Plasmid

#85626

Purpose

GST tagged Munc18c

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Promoter

CMV

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE- TRE-DEST-CBioTAP

Plasmid

#53569

Purpose

Lentiviral destination vector for C-Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Lentiviral

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pH6HTC_CCDC59-Halo

Plasmid

#175327

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCDC59 (CCDC59 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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S9_attL1-EGFP-C-term-attL2

Plasmid

#128473

Purpose

EGFP entry clone

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

EGFP entry clone

Expression

Bacterial

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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