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pX330-HypaCas9

Plasmid

#108302

Purpose

pX330 (Plasmid #42230) with the N692A, M694A, Q695A, and H698A mutations

Depositor

Yuichiro Miyaoka

Publication

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST26-C-FLAG

Plasmid

#79275

Purpose

Destination vector containing a C-terminal FLAG tag

Depositor

Rita Shiang

Publication

Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

Insert

FLAG

Expression

Mammalian

Promoter

CMV

Available since

March 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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MSGV Hu Acceptor PGK-NGFR

Plasmid

#64270

Purpose

Retroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFR

Depositor

David Ott

Publication

Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

Type

Empty backbone

Use

Retroviral

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pCM66

Plasmid

#45827

Depositor

Mary Lidstrom

Publication

Marx et al Microbiology. 2001 Aug;147(Pt 8):2065-75

Type

Empty backbone

Use

Broad host range cloning vector in bacteria

Promoter

lac

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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PMXS-SLC2A3

Plasmid

#72877

Purpose

expresses SLC2A3 in mammalian cells

Depositor

David Sabatini

Publication

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
AAV pTRE-FLEX-EGFP-WPRE-bGHpA

Plasmid

#65449

Purpose

Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

Depositor

Hongkui Zeng

Publication

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

EGFP

Use

AAV

Promoter

tetO

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAMPK gamma1

Plasmid

#15996

Depositor

Morris Birnbaum

Insert

AMPK gamma1 (Prkag1 Mouse)

Tags

His6

Expression

Mammalian

Available since

Oct. 26, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 EGFP-Sumo2

Plasmid

#13385

Depositor

Mary Dasso

Publication

Azuma et al J Cell Biol. 2003 Nov 10. 163(3):477-87.

Insert

Sumo2 (SUMO2 Human)

Tags

EGFP and GST

Expression

Bacterial

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pGP-AAV-CAG-FLEX-jGCaMP7b-WPRE

Plasmid

#104497

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

Depositor

Douglas Kim, GENIE Project

Publication

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Insert

jGCaMP7b

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p5E-Runx1+23

Plasmid

#69602

Purpose

Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

Depositor

Owen Tamplin, Leonard Zon

Publication

Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

Inserts

Runx1 +23 intronic enhancer (Runx1 Mouse)
Mouse beta-globin minimal promoter

Use

5' gateway entry vector

Promoter

C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

Available since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3-RAB-ICUE

Plasmid

#118480

Purpose

ddRFP-based single-color biosensor for monitoring cAMP dynamics.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-ICUE

Expression

Mammalian

Promoter

CMV

Available since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pT2/SV-Venus-SIINFEKL

Plasmid

#111624

Purpose

Sleeping Beauty transposon containing SV-40-driven Venus-LEQLE-SIINFEKL-TEW for antigen presentation studies

Depositor

Jon Yewdell

Insert

Venus-LEQLE-SIINFEKL-TEW

Tags

LEQLE-SIINFEKL-TEW

Expression

Mammalian

Promoter

SV40

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Myc-Nix

Plasmid

#100795

Purpose

Mammalian expression of Nix (Bnip3L)

Depositor

Joseph Gordon

Publication

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

BCL2 interacting protein 3 like (BNIP3L Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RasV12 in pENTR1A (w99-1)

Plasmid

#22252

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Expression

Mammalian

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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APJ-Tango

Plasmid

#66224

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Publication

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

APJ (APLNR Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-FNIP2

Plasmid

#72294

Purpose

overexpression

Depositor

David Sabatini

Publication

Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

Insert

FNIP2 (FNIP2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
mCherry-CyclinF

Plasmid

#32975

Depositor

Michele Pagano

Publication

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Tags

Flag and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
lacI-PT5-gusA-pBAV1k

Plasmid

#30501

Depositor

Ichiro Matsumura

Publication

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucuronidase (gusA Escherichia coli)

Tags

His

Expression

Bacterial

Available since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5M-TIMP3

Plasmid

#31715

Depositor

Rik Derynck

Publication

Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

Insert

TIMP3 (TIMP3 Human)

Tags

Myc

Expression

Mammalian

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Bmi1 shRNA1

Plasmid

#21576

Depositor

Sally Temple

Publication

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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