We narrowed to 719 results for: pgl3

1. pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

   Plasmid

   #198844

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   COX2-Q6-L18 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro

   Plasmid

   #173232

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-NLS-ccdb-DddA(G1333N)-UGI-Puro

   Plasmid

   #173233

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-NLS-ccdb-DddA(G1397C)-UGI-Puro

   Plasmid

   #173234

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-NLS-ccdb-DddA(G1397N)-UGI-Puro

   Plasmid

   #173235

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216246

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgAAVS1.5
   

   Use

   CRISPR; Human safe harbor locus (a avs1) site-spe…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-MTS-G3635A-L17-Q2L7-T2176N-UGI-Puro

   Plasmid

   #209647

   Purpose

   To install m.G3635 mutation on mtDNA

   Depositor

   Bin Shen

   Article

   Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

   Insert

   L17-Q2L7-T2176N
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216251

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgSEC61B.N1 (SEC61B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro

   Plasmid

   #209646

   Purpose

   To install m.G3635 mutation on mtDNA

   Depositor

   Bin Shen

   Article

   Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

   Insert

   L17-Q2L7-T2176C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro

    Plasmid

    #209642

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-FZY2-S100N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge