We narrowed to 6 results for: pgl3
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TypeBlog Post...widely used luciferase reporter gene plasmid pGL3 pGL3 luciferase reporter gene plasmids have been ... and Woods in the nineties. However, reliance on pGL3 has not been without problems due to the high rate...pXPG, which shows lower background expression than pGL3, enabling its use for the study of promoters with...pXPG contains the Luc+ luciferase gene derived from pGL3 but has a distinct advantage over the latter plasmid... the synthetic polyadenylation signal present in pGL3. This appears to contribute to more efficient blocking...
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Luciferase Plasmid Collection
TypeCollection...in our 27 Hot Plasmids from 2016 blog post. pGL3-lRF , pGL3-LRR : Genomic tiles spanning a region of interest...64784 pGL3-Basic-IRES Firefly Insertion of 5' promoter/enhancer regions Joshua Mendell 212936 pGL3 Basic...present for normalization Alejandro Ferrando 114670 pGL3-TK-5UTR-BsmBI-Luciferase Firefly Insertion of 5'...fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and renilla_polyA Firefly/Renilla Insertion... -
Sequencing Primers
TypeGuide...RVprimer3 CTAGCAAAATAGGCTGTCCC (Promega) 5' of MCS in pGL3 vector, forward primer SFFV-F ATTGATTGACTGCCCACCTC... -
Neurodegeneration Plasmid Collection
TypeCollection...Ristow 14978 pGL3-basic-hFX-promotor(1kb) FXN hFXN Friedreich ataxia Michael Ristow 14979 pGL3-basic-hFX-...Ristow 14980 pGL3-basic-hFX-promotor(2,5kb) FXN hFXN Friedreich ataxia Michael Ristow 14981 pGL3-basic-hFX-promotor...Yildiz 159787 pGL3-U6-pegRNA_KCNA1-EGFP KCNA1 U6 Episodic ataxia Xiaolong Wang 159788 pGL3-U6-sgRNA_KCNA1...Schroer 51435 pGL3Basic_ME.1/ApoEpromoter APOE ApoE Alzheimer's Sohail Tavazoie 51436 pGL3Basic-ME.2/ApoEpromoter... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Zhao pRB1017 59936 Worm BsaI none S. pyogenes Fire pGL3-U6-sgRNA-PGK-Puro 51133 Mammalian none S. pyogenes... -
Validated gRNA Sequences
TypeCollection...GGACTTTTGCCAGCCATGGT 52255 cut S. pyogenes 23929339 Sheen pGL3-Basic-8x-gRNA-eGFP reporter synthetic AAAGGTCGAGAAACTGCAAA...