We narrowed to 4,045 results for: golden gate
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Plasmid#199733PurposeGolden Gate entry vector; 7th gRNA under OsU6.2 promoterDepositorInsertOsU6.2-sgRNA7 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLsgRNA8
Plasmid#199734PurposeGolden Gate entry vector; 8th gRNA under TaU6 promoterDepositorInsertTaU6-sgRNA8 scaffold
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0013
Plasmid#117521PurposeLevel0 Golden Gate part: CDS, SpCas9-pDepositorInsertSpCas9-p (with stop codon)
UseCRISPRAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF002
Plasmid#48843PurposeProvides a BASTA resistance cassette as GreenGate module.DepositorInsertp35S::BastaR::t35S
UseGolden gate compatible cloning vectorMutationinternal BsaI recognition site in 35S promoter re…Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ147
Plasmid#69299PurposeGolden Gate recipient and Gateway entry vector; assembly of 7 gRNAsDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
BCJJ358
Plasmid#117505PurposeRPS5a:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:RPS5a:Cas9_4:E9:ttgc:BpiI
UseCRISPRExpressionPlantPromoterAtRPS5aAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
BCJJ344
Plasmid#117503PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2 reverseDepositorInsertBpiI:tagt:UBI10:Cas9-3(intron):E9:ttgc:BpiI
UseCRISPRExpressionPlantPromoterAtUBI10Available SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceExpressionWormPromoternot applicableAvailable SinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
L0-I18 Ub-Arg GG1-GG2
Plasmid#195899PurposeLevel 0 Ubiquitin-Arg tag, protein degration tag with GG1 GG2 golden gate linkersDepositorInsertUb-Arg tag
UseSynthetic BiologyAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPD355 VPR-FRB (NLS)
Plasmid#138847PurposeConstitutive expression of VPR-FRB under the CMV promoter.DepositorInsertVPR-FRB
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
L0-I39 PhiC31-Intein GG1-GG4
Plasmid#195905PurposeLevel 0 PhiC31-intein(DnaE-Npu) with GG1 and GG4 golden gate linkersDepositorInsertPhiC31-intein
UseSynthetic BiologyAvailable SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I40 Intein-PhiC31 GG1-GG4
Plasmid#195906PurposeLevel 0 Intein(DnaE-Ssp)-PhiC31 with GG1 and GG4 golden gate linkersDepositorInsertIntein-PhiC31
UseSynthetic BiologyAvailable SinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
BCJJ340
Plasmid#117502PurposeUBI10:Cas9:E9 Golden Gate Level 1 Position 2DepositorInsertBpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
UseCRISPRExpressionPlantPromoterAtUBI10Available SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGF008
Plasmid#48848PurposeProvides a BASTA resistance cassette as GreenGate module.DepositorInsertpNOS::BastaR::tNOS
UseGolden gate compatible cloning vectorMutationchi sequence in BastaR removed by silent substitu…Available SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLY134
Plasmid#130962PurposeEngineered sgRNA-LEB5 generator including Anderson promoter J23100 for golden gate assemblyDepositorInsertsgRNA-LEB5
UseSynthetic BiologyExpressionBacterialPromoterAnderson promoter J23100Available SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0058
Plasmid#117539PurposeLevel1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (SaCas9)
ExpressionPlantAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZA55
Plasmid#174768PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E without STOP codon
UseUnspecifiedMutationL17EAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA56
Plasmid#174769PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-L17E/D481V without STOP codon
UseUnspecifiedMutationL17E/D481VAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA54
Plasmid#174767PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn-D481V without STOP codon
UseUnspecifiedMutationD481VAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA53
Plasmid#174766PurposeEntry clone for Golden Gate assemblyDepositorInsertGG_NbZAR1syn without STOP codon
UseUnspecifiedMutationWTAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD
Plasmid#162810PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expressionDepositorInsertBsmBI_Intein_DD
UsePiggybacExpressionMammalianPromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH184
Plasmid#122857PurposeMS2-modified guide RNA targeting the FT promoter with GreenGate E-F flanking sequencesDepositorInsertMS2-modified sgRNA targeting the Arabidopsis FT promoter
UseGolden gate compatible cloning vectorAvailable SinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0007
Plasmid#117534PurposeLevel0 Golden Gate part: CDS, LbCas12aDepositorInsertLbCas12a (with stop codon)
UseCRISPRAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGD007
Plasmid#48837PurposeProvides linker:nuclear localization signal as GreenGate C-terminal tag module.DepositorInsertlinker:nuclear localization signal
UseGolden gate compatible cloning vectorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0021
Plasmid#117532PurposeLevel0 Golden Gate part: CDS, SaCas9 (no stop codon)DepositorInsertSaCas9 (no stop codon)
UseCRISPRAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0016
Plasmid#117526PurposeLevel0 Golden Gate part: CDS, Sp-eCas9 1.0DepositorInsertSp-eCas9 1.0 (with stop codon)
UseCRISPRMutationK810A, K1003A, R1060AAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
EML6442_p35S-mCherry2-SV40
Plasmid#234351PurposeExpressed mCherry2 florescent proteins in plant cells for tracing transformation eventsDepositorInsertSpeR
ExpressionPlantAvailable SinceNov. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
DHFR-TEV (integrating)
Plasmid#247218PurposePlasmid for genomic integration of DHFR-TEV into the YORWΔ22 locus of yeast cells.DepositorInsertDHFR-TEV
ExpressionYeastPromoterTEF2Available SinceNov. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
VN1272 pB2Hws_v28_Ddcm_wkKanR_TIR51_pLWeakRBS_TIR371_cI-2GG+rpoA-IP3
Plasmid#235120PurposeQuantitative bacterial two-hybrid (qB2H). Expresses IP3.DepositorTypeEmpty backboneUseSynthetic Biology; Quantitative bacterial two-hyb…TagsrpoAExpressionBacterialAvailable SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
L3928 Filler DNA
Plasmid#244193PurposeEmpty vector; modified pcDNA with CMV promoter and bGHpolyA removed to reduce burdenDepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianAvailable SinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
p0AmpCD
Plasmid#237276PurposeEmpty vector for L0 TypeIISDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
p0AmpAB
Plasmid#237274PurposeEmpty vector for L0 TypeIISDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceSept. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tad-CBE2.1
Plasmid#235053PurposeExpresses Tad-CBE2.1 in mammalian cells.DepositorInsertTad-CBE2.1-SpCas9 D10A
UseCRISPRTags2xUGI, SV40NLS and bpNLSExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tad-CABE2.5
Plasmid#235054PurposeExpresses Tad-CABE2.5 in mammalian cells.DepositorInsertTad-CABE2.5-SpCas9 D10A
UseCRISPRTags2xUGI, SV40NLS and bpNLSExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tad-CBE3.1
Plasmid#235056PurposeExpresses Tad-CBE3.1 in mammalian cells.DepositorInsertTad-CBE3.1-SpCas9 D10A
UseCRISPRTags2xUGI, SV40NLS and bpNLSExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tad-CBE3.2
Plasmid#235057PurposeExpresses Tad-CBE3.2 in mammalian cells.DepositorInsertTad-CBE3.2-SpCas9 D10A
UseCRISPRTags2xUGI, SV40NLS and bpNLSExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tad-CABE1.1
Plasmid#235051PurposeExpresses Tad-CABE1.1 in mammalian cells.DepositorInsertTad-CABE1.1-SpCas9 D10A
UseCRISPRTags2xUGI, SV40NLS and bpNLSExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tad-CABE1.2
Plasmid#235052PurposeExpresses Tad-CABE1.2 in mammalian cells.DepositorInsertTad-CABE1.2-SpCas9 D10A
UseCRISPRTags2xUGI, SV40NLS and bpNLSExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTE5425
Plasmid#229032PurposeExpression vector for 10xHis-phi29 DNA polymerase (catalytically inactive mutant D249E): PT7-lacO-g10leader-p2 dead-T7 terminator, CamRDepositorInsertPT7-lacO-g10lead-10xHisTag-phi29 DNAP (D249E, catalytically inactive)
Tags10x His-tag + Factor Xa recognition siteExpressionBacterialPromoterPT7-lacO and T7 promoterAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSWITCH
Plasmid#222402PurposeExpression in Streptococcus pyogenesDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 2, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
Hv_HPT:pCSRM2C:Gus_introns
Plasmid#215191PurposeEvaluating guard cell specific promotors in BarleyDepositorInsertScream2
PromoterBdSCRM2Available SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bo_NPTII:pCSRM2C:Gus_introns
Plasmid#215177PurposeEvaluating guard cell specific promotors in BrassicaDepositorInsertScream2
UseSynthetic BiologyPromoterpSCRM2Available SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRO288
Plasmid#233827PurposeMoClo-YTK; type4A; Activation domains; VP48DepositorInsertVP48
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRO129
Plasmid#233805PurposeMoClo-YTK; type3; rtTA systems; rtTA3DepositorInsertrtTA3
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRO232
Plasmid#233808PurposeMoClo-YTK; type2A; ABA systems; phlfO7DepositorInsertphlfO7
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRO175
Plasmid#233810PurposeMoClo-YTK; type3B; ABA systems; ABIDepositorInsertABI
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRO177
Plasmid#233811PurposeMoClo-YTK; type3B; ABA systems; PYL1DepositorInsertPYL1
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRO286
Plasmid#233812PurposeMoClo-YTK; type2A; Auxin systems; amtRO7DepositorInsertamtRO7
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN022
Plasmid#233814PurposeMoClo-YTK; type3B; Auxin systems; AIDΔ34DepositorInsertAIDΔ34
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWN079
Plasmid#233819PurposeMoClo-YTK; type3B; NS3 systems; NS3aDepositorInsertNS3a
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only