We narrowed to 4,784 results for: AAT
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Plasmid#224069PurposeBacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage siteDepositorInserttlnrd1 (TLNRD1 Human)
UseTags6xHISExpressionBacterialMutationL191T/A225T double mutant; reduces affinity of TL…PromoterT7Available sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
TLNRD1(4H)-2E_pET151
Plasmid#224068PurposeBacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage siteDepositorInserttlnrd1 (TLNRD1 Human)
UseTags6xHIS; V5ExpressionBacterialMutationK192E/R233E double mutant; prevents actin binding…PromoterT7Available sinceAug. 26, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
AA244
Plasmid#215945PurposeFragmid fragment: (guide cassette) guide expression; positive control cell surfaceDepositorHas ServiceCloning Grade DNAInsertU6_v1; sgCD59_v1 [Sp]; trRNA_v4 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA-pcr4
Plasmid#193662PurposeExpression of tandem pre-sgRNA array pcr4 for LbCas12aDepositorInsertU6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I432D_eGFP
Plasmid#208879PurposeExpress eGFP-CCM2 with the I432D mutation in mammalian cells.DepositorInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutation I432DPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_W421AD422A_eGFP
Plasmid#208880PurposeExpress eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.DepositorInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutations W421A and D422APromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I428S_eGFP
Plasmid#208878PurposeExpress eGFP-CCM2 with the I428S mutation in mammalian cells.DepositorInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutation I428SPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
LENTICRISPR-PGM2_sgRNA1
Plasmid#201616PurposeCRISPR/Cas9-mediated gene knock-outDepositorInsertPGM2 (PGM2 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1B CDC25A_2
Plasmid#160768PurposeSuppress CDC25ADepositorInsertshCDC25A_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon3_1_As
Plasmid#155057PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon2_2_As
Plasmid#155056PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLCHKO_HPRT1_exon2_2_Lb
Plasmid#155052PurposeLentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)DepositorInsert(hg)RNA for deletion of HPRT1 exon 2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-Sfxn2 (CG6812)
Plasmid#110643PurposeRetroviral expression of Flag-tagged Drosophila CG6812 in mammalian cellsDepositorInsertCG6812 (CG6812 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-Sfxn1-3 (CG11739)
Plasmid#110642PurposeRetroviral expression of Flag-tagged Drosophila CG11739 in mammalian cellsDepositorInsertCG11739 (CG11739 Fly)
UseRetroviralTagsFLAGExpressionMammalianMutationcodon-optimized cDNA (no amino acid mutations)PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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RPS6KL1 gRNA (BRDN0001487111)
Plasmid#77954Purpose3rd generation lentiviral gRNA plasmid targeting human RPS6KL1DepositorInsertRPS6KL1 (RPS6KL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PDGFRL gRNA (BRDN0001147755)
Plasmid#77698Purpose3rd generation lentiviral gRNA plasmid targeting human PDGFRLDepositorInsertPDGFRL (PDGFRL Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PIP4K2C gRNA (BRDN0001148338)
Plasmid#77681Purpose3rd generation lentiviral gRNA plasmid targeting human PIP4K2CDepositorInsertPIP4K2C (PIP4K2C Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SRPK1 gRNA (BRDN0001149313)
Plasmid#77494Purpose3rd generation lentiviral gRNA plasmid targeting human SRPK1DepositorInsertSRPK1 (SRPK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only