Skip to main content
Addgene
Showing: 861 - 880 of 1075 results

  1. HINT2-bio-His

    Plasmid
    #53406
    Purpose
    Expresses full-length Histidine triad nucleotide-binding protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    HINT2 (HINT2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53406-plasmid-map-sequence-id-83184

    Enlarge

  2. CCDC-bio-His

    Plasmid
    #53331
    Purpose
    Expresses full-length Coiled-coil domain-containing protein 109A precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    CCDC (MCU Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53331-plasmid-map-sequence-id-83062

    Enlarge

  3. G6f-bio-His

    Plasmid
    #53342
    Purpose
    Expresses full-length Lymphocyte antigen 6 complex locus protein G6f precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    G6f (LY6G6F Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53342-plasmid-map-sequence-id-83072

    Enlarge

  4. DCBL-bio-His

    Plasmid
    #51867
    Purpose
    Expresses full-length Discoidin, CUB and LCCL domain-containing protein 2 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    DCBL (DCBLD2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51867-plasmid-map-sequence-id-79763

    Enlarge

  5. ESAM-bio-His

    Plasmid
    #51751
    Purpose
    Expresses full-length Endothelial cell-selective adhesion molecule precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ESAM (ESAM Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51751-plasmid-map-sequence-id-79336

    Enlarge

  6. ACVR1-bio-His

    Plasmid
    #51623
    Purpose
    Expresses full-length Activin receptor type-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ACVR1 (ACVR1 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51623-plasmid-map-sequence-id-78913

    Enlarge

  7. pRDA_936

    Plasmid
    #216094
    Purpose
    Cas12a [EnAs] knockout targeting CD46, CD47, CD55, CD81, positive control
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Insert
    CD46, CD47, CD55, CD81 guides
    Use
    CRISPR and Lentiviral; Positive controls
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216094-plasmid-map-sequence-id-431378

    Enlarge

  8. IL6ST-bio-His

    Plasmid
    #51806
    Purpose
    Expresses full-length Interleukin-6 receptor subunit beta precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    IL6ST (IL6R Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51806-plasmid-map-sequence-id-79529

    Enlarge

  9. APP-bio-His

    Plasmid
    #51643
    Purpose
    Expresses full-length Amyloid beta A4 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    APP (APP Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51643-plasmid-map-sequence-id-78965

    Enlarge

  11. pcDNA3.1/hpa5(aIIB_thigh)

    Plasmid
    #59971
    Purpose
    integrin a5 chimeric headpiece expression (a5 beta-propeller domain and aIIB thigh domain)
    Depositor
    Timothy Springer
    Insert
    human integrin a5 beta-propeller domain fused with human integrin aIIB thigh domain (ITGA5, ITGA2B Human)
    Use
    Tags
    Acid coiled-coil and StrepII tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. MSNA-c028

    Plasmid
    #175470
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.
    Depositor
    Opher Gileadi
    Article
    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    H288A
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175470-plasmid-map-sequence-id-353161

    Enlarge

  13. MSNA-c021

    Plasmid
    #175468
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44.
    Depositor
    Opher Gileadi
    Article
    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    L281R
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175468-plasmid-map-sequence-id-353157

    Enlarge

  14. MSNA-c029

    Plasmid
    #175471
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R and H288A point mutations that reduce binding to CD44.
    Depositor
    Opher Gileadi
    Article
    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    L281R, H288A
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175471-plasmid-map-sequence-id-353253

    Enlarge

  15. pMXs-IP-mCherry-TOSI

    Plasmid
    #172496
    Purpose
    mCherry-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    PDCD4 (Pdcd4 Mouse)
    Use
    Retroviral
    Tags
    Mcherry
    Expression
    Mammalian
    Mutation
    Promoter
    MLV LTR
    Available since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172496-plasmid-map-sequence-id-338550

    Enlarge

  16. pMXs-IP-mVenus-TOSI

    Plasmid
    #172491
    Purpose
    mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.
    Depositor
    David Scadden
    Article
    Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:
    Insert
    Pdcd4 (Pdcd4 Mouse)
    Use
    Retroviral
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    MLV LTR
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    172491-plasmid-map-sequence-id-346537

    Enlarge

  17. GRP94-COMP-blac-flag-his

    Plasmid
    #111008
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    endoplasmin, putative (GRP94) (PFL1070c Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111008-plasmid-map-sequence-id-224562

    Enlarge

  18. AAP-COMP-blac-flag-his

    Plasmid
    #111001
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    aminopeptidase P (APP) (PF14_0517 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    111001-plasmid-map-sequence-id-224546

    Enlarge

  19. PF3D7_1310500-COMP-blac-flag-his

    Plasmid
    #110967
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    conserved Plasmodium protein (PF3D7_1310500 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110967-plasmid-map-sequence-id-224243

    Enlarge

  20. PF3D7_1242000-COMP-blac-flag-his

    Plasmid
    #111025
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Article
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    conserved Plasmodium protein (PF3D7_1242000 Synthetic)
    Use
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    111025-plasmid-map-sequence-id-224709

    Enlarge

Showing: 861 - 880 of 1075 results