We narrowed to 4,783 results for: Drosophila

Showing: 861 - 880 of 4783 results

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pAc5.1B-EGFP-DmRRP40

Plasmid

#65797

Purpose

expresses EGFP-tagged RRP40 in Drosophila cells

Depositor

Elisa Izaurralde

Insert

RRP40 (Rrp40 Fly)

Use

Drosophila expression

Tags

EGFP

Promoter

Actin 5C

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pET-26b-Nb127D01-ALFA-His

Plasmid

#171568

Purpose

Bacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

Nb127D01-ALFA-His (CXCR2 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-dVP16AD-Hsp70

Plasmid

#62908

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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hs-cre::EBD, UAS-dBrainbow attB

Plasmid

#220326

Purpose

expresses a heat-shock inducible cre::EBD fusion protein and UAS-dBrainbow

Depositor

Erdem Bangi

Inserts

Cre::EBD (with intron)
dBrainbow

Expression

Insect

Promoter

UAS and hs

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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UAS-sulfateless

Plasmid

#41174

Depositor

Norbert Perrimon

Article

Lin et al Nature. 1999 Jul 15;400(6741):281-4.

Insert

sulfateless (sfl Fly)

Expression

Insect

Promoter

Hsp70

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only
pWALIUM-intron 3x UAS attB

Plasmid

#206360

Purpose

includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

Depositor

Erdem Bangi

Article

Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP

Plasmid

#219639

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAW-NbVHH05-mCherry

Plasmid

#171572

Purpose

Expresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-mCherry (UBE2J1 Human)

Tags

mCherry

Expression

Insect

Promoter

fly actin5C promoter

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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hMTP

Plasmid

#124281

Purpose

Expresses human MTP in mammalian cells.

Depositor

Mahmood Hussain

Article

Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

Insert

MTTP (MTTP Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pF3BGX

Plasmid

#138387

Purpose

Empty RMCE vector (phiC31 attB/loxP)

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

P5' FRT3 3xP3-Venus φC31-attB loxP FRT3 P3'

Expression

Insect

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal80-3xP3-RFP

Plasmid

#127562

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal80-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal80

Use

CRISPR

Expression

Insect

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-Gal4DBD-3xP3-RFP

Plasmid

#127564

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

Gal4DBD

Use

CRISPR

Expression

Insect

Available Since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJAT27

Plasmid

#204308

Purpose

For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-GFPc

Use

CRISPR

Promoter

MHC

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJAT18

Plasmid

#204307

Purpose

For generating targeting inversions. N-terminal split GFP, expressed in flight muscles.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

MHC-GFPn

Use

CRISPR

Promoter

MHC

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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OA-1043

Plasmid

#164586

Purpose

clone in U6 and DR

Depositor

Omar Akbari

Article

Sun et al J Vis Exp. 2021 Feb 5;(168). doi: 10.379

Insert

U6 and DR

Expression

Insect

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pRB31

Plasmid

#72868

Purpose

template for N-terminal 1x Strep tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

single Strep tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRB30

Plasmid

#72860

Purpose

template for N-terminal eGFP tag, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

eGFP

Expression

Insect

Mutation

eGFP

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pKF292

Plasmid

#72856

Purpose

template base vector for N-terminal tagging, inducible Blasticidin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for Nterm-tag templates

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab4 CA

Plasmid

#53466

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab04

Tags

YFP

Expression

Insect

Mutation

Q67L

Promoter

GAL4

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pUASP YFP Rab35 WT

Plasmid

#37741

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab35 (Rab35 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only