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Plasmid#200963Purpose3rd gen lentiviral plasmid for expressing mitochondria localized TurboID with EGFPDepositorInsertMito-TurboID
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EGFP-IRES-Neo
Plasmid#128660Purposemammalian expression of EGFP with neomycin selectionDepositorInsertEGFP
UseLentiviralTagsN/AExpressionMammalianMutationWild typePromoterCMVAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_LKB1
Plasmid#108111PurposeLentiviral expression plasmid of human LKB1 cDNA with neomycin resistance geneDepositorInsertLKB1 (STK11 Human)
UseCRISPR and LentiviralTagsFLAGExpressionMammalianMutationPromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-Rac1-2G
Plasmid#66110PurposeSecond generation Rac1 FRET biosensorDepositorInsertSecond generation Rac1 FRET biosensor
UseTagsHis-MycExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEJS614_pTetR-P2A-BFPnls/sgNS
Plasmid#108650PurposeNon-specific Spy sgRNA under U6 promoterDepositorInsertsNon-specific sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUGW-Golgi-TurboID
Plasmid#200964Purpose3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFPDepositorInsertGolgi-TurboID
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-cilia-APEX
Plasmid#73186PurposeExpresses cilia-APEX in mammalian cellsDepositorInsertcilia-APEX (Nphp3 Glycine max, Mouse)
UseTagsinternal GFP tagExpressionMammalianMutationPromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-TAG-ERN
Plasmid#80476PurposeAll-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)DepositorTypeEmpty backboneUsePiggybac transposonTagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
Plasmid#196192Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locusDepositorInsert2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
UseZnf donorTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mito-TurboID
Plasmid#200960PurposeTransiently expresses mitochondria localized TurboID with V5 tagsDepositorInsertmito-TurboID
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSirt3-RFP
Plasmid#216806PurposeHuman Sirt3-RFP driven by the CaMKII promoterDepositorInsertSirt3 (SIRT3 Human)
UseLentiviralTagsmRFP1ExpressionMammalianMutationPromoterCamKIIAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-BioID2-BioID2-HA
Plasmid#176740PurposeExpresses the promiscuous small biotin ligase, BioID2, in astrocytesDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHPHS232
Plasmid#228356PurposeLanding pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locusDepositorInsertsmTagBFP2
Reverse tetracycline transactivator 3
neo
UseTagsBlasticidin S deaminase and iCasp9ExpressionMammalianMutationPromoterTRE3GV and gene trapAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-3FLAG-RAB5A
Plasmid#176488PurposeFor lentiviral transduction of 3FLAG-RAB5ADepositorInsertRAB5A (RAB5A Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLG-bio-His
Plasmid#53427PurposeExpresses full-length Plasminogen precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPLG (PLG Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-LifeAct-GFP
Plasmid#190199PurposeExpresses the small actin binding peptide, LifeAct, with a GFP reporterDepositorInsertLifeAct-eGFP
UseAAVTagsGFPExpressionBacterial and MammalianMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 GfaABC1D rM3D mCherry SV40
Plasmid#92285PurposeAAV DREADD expressionDepositorInsertrM3D
UseAAVTagsmCherryExpressionMutationPromoterGfaABC1DAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-BioID2-BioID2-HA
Plasmid#176855PurposeExpresses the promiscuous small biotin ligase, BioID2, in neuronsDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEJS613_pTetR-P2A-BFPnls/sgTelo
Plasmid#108649PurposeTelomere-targeting Spy sgRNA under U6 promoterDepositorInsertsTelomere-targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianMutationPromoterU6 and hPGKAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-GFP
Plasmid#177333PurposeExpresses GFP on the plasma membrane of neurons via an Lck localization sequenceDepositorInsertLck-GFP
UseAAVTagsGFPExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only