We narrowed to 1,198 results for: e.coli
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Plasmid#220133PurposeExpresses dCas9-FCPF in E.coliDepositorInsertdCas9-FCPF (cas9 S. pyogenes, Synthetic)
Tags6xHisExpressionBacterialMutationFCPF sequence inserted on C-terminalPromoterT7Available SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWzyE-His
Plasmid#196669PurposeExpression of C-terminally His10-tagged WzyE from Pectobacterium atrosepticum in E.coliDepositorInsertWzyE
TagsTEV cleavable His10ExpressionBacterialPromoteraraBADAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2061
Plasmid#186299PurposeExpresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoterDepositorInsertsLexA-HBD-B112
Cas9
UseCRISPR and Synthetic BiologyExpressionBacterial and YeastPromoter2xLexop-minimalCyc1 and PACT1Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R1CiM_4EGFP_R3EGFPi2
Plasmid#185968PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.DepositorInsertMolecular IMPLY gate
UseSynthetic BiologyAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R3tdTomatoi2
Plasmid#185969PurposeExpresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.DepositorInsertPr-i2rbs-tdTomato cassette in reverse direction in Network Brick
UseSynthetic BiologyPromoterPRAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-UTX(420-633)-H6
Plasmid#183773PurposeExpress pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.DepositorInsertUTX
Tags6 His tag at the C-terminusExpressionBacterialPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
DT033A
Plasmid#159394PurposeConstitutive murine mature BDNF expression, controlled under E.coli tac promoterDepositorInsertpr.tac-BDNF (Bdnf, Mouse) (Bdnf Mouse)
UseSynthetic BiologyTagsFLAGExpressionBacterialPromotertac promoterAvailable SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_3H1L_02.395
Plasmid#155198PurposeExpresses 3H1L_02.395 in E.coliDepositorInsert3H1L_02.395
Tags6x His TagExpressionBacterialAvailable SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97E
Plasmid#125825PurposeExpresses GST-RPN13 (19-132) K97E in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged lysine 97 to glutamatePromoterT7Available SinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K83A
Plasmid#125829PurposeExpresses GST-RPN13 (19-132) K83A in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged lysine 83 to alaninePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97A
Plasmid#125823PurposeExpresses GST-RPN13 (19-132) K97A in E.coliDepositorAvailable SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97D
Plasmid#125824PurposeExpresses GST-RPN13 (19-132) K97D in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged lysine 97 to aspartatePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104A
Plasmid#125826PurposeExpresses GST-RPN13 (19-132) R104A in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged arginine 104 to alaninePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104D
Plasmid#125827PurposeExpresses GST-RPN13 (19-132) R104D in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged arginine 104 to aspartatePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) R104E
Plasmid#125828PurposeExpresses GST-RPN13 (19-132) R104E in E.coliDepositorInsertRPN13 (ADRM1 Human)
TagsGSTExpressionBacterialMutationchanged arginine 104 to glutamatePromoterT7Available SinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-C-SH2
Plasmid#111419Purposeexpress murine Syk (C)SH2 domain (His 162 to Gln 264), in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (C)SH2 domain (His 162 to Gln 264), without any tag (Syk Mouse)
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-30a(+)-N-SH2
Plasmid#111274Purposeexpress murine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site, in E.coli strain Rosetta 2 (DE3)DepositorInsertmurine Syk (N)SH2 domain (Ser 8 to Gly 117), with an N-terminal (His)6 tag and TEV cleavage site (Syk Mouse)
TagsHHHHHHENLYFQGExpressionBacterialPromoterT7 promoterAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-953)
Plasmid#73743PurposeExpression of GFP-PP-RPN2 (918-53) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (932-953)
Plasmid#73745PurposeExpression of GFP-PP-RPN2 (932-953) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 932-953 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-GFP-PP-RPN2 (918-947)
Plasmid#73747PurposeExpression of GFP-PP-RPN2 (918-947) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
Tags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-947 of RPN2PromoterT7Available SinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only