We narrowed to 12,197 results for: 112

1. GST-Delta23C11orf83

   Plasmid

   #65849

   Purpose

   bacterial expression of GST (N-ter) - Delta 23 C11orf83/UQCC3 (UQCC3 protein depleted of its N-terminal transmembrane domain)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   UQCC3 depleted of its N terminal transmembrane part (UQCC3 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion of the 23 first aa (TM)

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CoV2-N-P199L

   Plasmid

   #177949

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P199L). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P199L) (N )
   

   Expression

   Mammalian

   Mutation

   P199L

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. IKK2-EGFP

   Plasmid

   #111195

   Purpose

   fluorescent fusion protein

   Depositor

   Johannes A. Schmid

   Article

   Birbach et al J Biol Chem. 2002 Mar 29;277(13):10842-5

   Insert

   IKKbeta (IKBKB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   June 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. NSP6-FR-2Strep

   Plasmid

   #210317

   Purpose

   Expresses SARS-CoV-2 NSP6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-p3-PE(1153-dRnH)-C-term

   Plasmid

   #187182

   Purpose

   p3-PE2-C-term(1153-dRnH) in AAV2 backbone (ITRs) targeting DNMT1

   Depositor

   Gerald Schwank

   Article

   Bock et al Sci Transl Med. 2022 Mar 16;14(636):eabl

   Insert

   PE2 from plasmid #132775 with shortened RnaseH domain
   

   Use

   AAV

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sc++

   Plasmid

   #155011

   Purpose

   Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

   Depositor

   Joseph Jacobson

   Article

   Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

   Insert

   Sc++
   

   Expression

   Mammalian

   Mutation

   T1227K, Loop Substitution from S. anginosus

   Promoter

   EF1ɑ

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

   Plasmid

   #89149

   Purpose

   An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

   Insert

   hM3D(Gq)
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-AID-BLM-UGI

   Plasmid

   #221218

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-BLM-UGI
   

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pADSL-EGFP

   Plasmid

   #99109

   Purpose

   Expresses human ADSL-EGFP fusion in mammalian cells

   Depositor

   Stephen Benkovic

   Article

   An et al Science. 2008 Apr 4;320(5872):103-6. doi

   Insert

   ADSL (ADSL Human)
   

   Tags

   EGFP (enhanced green fluorescent protein)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Syn-Ace-mNeon2

    Plasmid

    #195518

    Purpose

    Green fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    Ace-mNeon2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    Ace-mNeon 81S; SY linker

    Promoter

    Synapsin

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDarmo.CMVT_v1

    Plasmid

    #133072

    Purpose

    Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

    Depositor

    Kacper Rogala, David Sabatini

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-Flag-TwinStrep and TwinStrep-Flag-3C

    Expression

    Mammalian

    Promoter

    CMVT (Enhanced CMV)

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLX_307 SLC31A1

    Plasmid

    #184491

    Purpose

    Lentivirus expression for SLC31A1 gene

    Depositor

    Peter Tsvetkov, Todd Golub

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    SLC31A1 (SLC31A1 Human)
    

    Use

    Lentiviral

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZR040_Lenti-U6-sgCtrl-MS2-PP7-hPGK-PCP-p65-HSF1-Puro

    Plasmid

    #180266

    Purpose

    Lentiviral expression vector for CRISPRa-SAM with non-targeting control sgRNA

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    No-Target Control sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-VARNAM2-Kv2.1PR

    Plasmid

    #195524

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    Synapsin

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. C1-mCitrine-FKBP-EZRabd(t567D)

    Plasmid

    #155227

    Purpose

    Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system
    

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Changed Thr 567 to Asp in original Ezrin

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

    Plasmid

    #194678

    Purpose

    CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    CHRM3_cpHalo∆
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBluescript II KS(+) EACMCV DNA-A 1.6mer

    Plasmid

    #159136

    Purpose

    Infectious clone containing a partial-tandem-dimer of East African cassava mosaic Cameroon virus 1998 DNA-A

    Depositor

    Vincent Fondong

    Article

    Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

    Insert

    EACMCV DNA-A 1.6mer
    

    Use

    Infectious clone for bombarding plants

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CaMKIIa-mScarlet

    Plasmid

    #131000

    Purpose

    AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Has Service

    AAV8

    Insert

    mScarlet
    

    Use

    AAV

    Promoter

    CaMKIIa

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CCT8_pCSdest

    Plasmid

    #53795

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Insert

    CCT8 (CCT8 Human)
    

    Expression

    Mammalian

    Promoter

    SP6 and CMV

    Available Since

    Sept. 9, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

    Plasmid

    #110560

    Purpose

    miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    stRBS-lacZ
    

    Expression

    Bacterial

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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