We narrowed to 10,032 results for: ADA

1. pXR004_puroR

   Plasmid

   #219820

   Purpose

   hU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)

   Depositor

   Philipp Maass

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAS9id_TRP

   Plasmid

   #141254

   Purpose

   Galactose-inducible expression of Cas9 for addressing two targets; Contains two guide RNA expression cassettes with stuffer and Kpn restriction sites.

   Depositor

   Adam Bertl

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   GAL1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_CMV_FR-GECO1c

   Plasmid

   #224358

   Purpose

   Expresses red fluorescent calcium sensor FR-GECO1c

   Depositor

   Adam Cohen

   Article

   Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

   Insert

   FR-GECO1c
   

   Use

   Lentiviral

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mEmerald-Homer1-C-18

   Plasmid

   #54120

   Purpose

   Localization: Membrane Adaptor, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Homer1 (Homer1 Rat)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. AAVS1-SAGFP

   Plasmid

   #127100

   Purpose

   SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.

   Depositor

   Adam Karpf

   Insert

   SA-GFP reporter
   

   Expression

   Mammalian

   Available Since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEmerald-Homer1-N-18

   Plasmid

   #54121

   Purpose

   Localization: Membrane Adaptor, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Homer1 (Homer1 Rat)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. PZ055:mOrange2-TM

   Plasmid

   #186430

   Purpose

   mOrange2-TM

   Depositor

   Adam Cohen

   Article

   Shi et al Cell. 2018 Dec 13;175(7):1769-1779.e13.

   Insert

   mOrange2-TM
   

   Expression

   Mammalian

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-dCas9_puroR

   Plasmid

   #219825

   Purpose

   CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)

   Depositor

   Philipp Maass

   Insert

   dCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A/H841A

   Promoter

   CMV

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PS-CFP2-H2B-6

   Plasmid

   #57228

   Purpose

   Localization: Membrane Adaptor, Excitation: 400 / 490, Emission: 468 / 511

   Depositor

   Michael Davidson

   Insert

   H2B (H2BC11 Human)
   

   Tags

   PS-CFP2

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pTJK482

    Plasmid

    #138001

    Purpose

    GATA1 expression. Vector also expresses GFP under control of hUBC promoter

    Depositor

    Tami Kingsbury

    Insert

    GATA1 (GATA1 Human)
    

    Use

    Lentiviral

    Mutation

    None

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSkunk3-BLA

    Plasmid

    #61531

    Purpose

    Phagemid expressing streptomycin/spectinomycin resistance gene

    Depositor

    Marc Ostermeier

    Article

    Firnberg et al PLoS One. 2012;7(12):e52031. doi: 10.137

    Insert

    aadA
    

    Expression

    Bacterial

    Promoter

    lacI

    Available Since

    Dec. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBEST-p15a-pBAD-TetO1-UTR1-deGFP-ssrA-T500

    Plasmid

    #45788

    Depositor

    Richard Murray

    Insert

    deGFP
    

    Use

    Synthetic Biology

    Tags

    ssrA degradation tag

    Expression

    Bacterial

    Promoter

    pBAD-tetO1

    Available Since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTJK637

    Plasmid

    #138010

    Purpose

    GATA1 knockout

    Depositor

    Tami Kingsbury

    Insert

    GATA1 sgRNA (GATA1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. EP003: CAG::GEM7-HaloTag

    Plasmid

    #209303

    Purpose

    Expression plasmid for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice via in utero electroporation

    Depositor

    Adam Cohen

    Insert

    GEM7
    

    Use

    AAV

    Tags

    HaloTag

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. EP004: pAAV TRE::GEM7-HaloTag

    Plasmid

    #209304

    Purpose

    Doxycycline-gated expression plasmid (TetOFF) for GEM7 (encapsulin from R. rubrum) with C-terminal HaloTag suitable for expression in mice

    Depositor

    Adam Cohen

    Insert

    GEM7
    

    Use

    AAV

    Tags

    HaloTag

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCW57.1-GFP_mmu-miR-16-5p_sponge

    Plasmid

    #132624

    Purpose

    Expresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTR

    Depositor

    Constance Ciaudo

    Insert

    stop codon, mmu-miR-16-5p sponge sequence and polyA signal
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW57-MCS1-P2A-MCS2 (Neo)

    Plasmid

    #89180

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. dCas9-miniTurbo

    Plasmid

    #219822

    Purpose

    EF1α-driven expression of dCas9 fused to miniTurbo with GFP (Adapted from plasmid #153209)

    Depositor

    Philipp Maass

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    miniTurbo

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    EF1a

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. mGeos-M-Homer1-C-18

    Plasmid

    #57528

    Purpose

    Localization: Membrane Adaptor, Excitation: 503 / 569, Emission: 514 / 581

    Depositor

    Michael Davidson

    Insert

    Homer1 (Homer1 Rat)
    

    Tags

    mGeos-M

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct

    Plasmid

    #212705

    Purpose

    targeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) system

    Depositor

    Elzo de Wit

    Insert

    ZFP143-FKBPV-2HA-P2A-GFP
    

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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