We narrowed to 168,189 results for: addgene

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pTCO2-mTDGi.1

Plasmid

#149430

Purpose

miniTdg gene, catalytic mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

N151A catalytic mutant, SNPs of Tdg gene in OLA/1…

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-Myosin7B-sgRNA

Plasmid

#154861

Purpose

Lentiviral expression of Cas9 and sgRNA targeting Myosin7B

Depositor

Yihong Ye

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

Insert

MYO7B sgRNA (MYO7B Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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MEN2A-MAC

Plasmid

#139634

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

MEN2A (RET Human)

Tags

StrepIII-HA-BirA*

Expression

Mammalian

Mutation

C634W

Promoter

CMV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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Y365A or A365Y inducible rescue

Plasmid

#131323

Purpose

gRNA to generate i-WT-r or i-MT-r systems

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

Y365A or A365Y inducible rescue

Use

CRISPR

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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MTF2_KO_ex11_gRNA-4

Plasmid

#131334

Purpose

gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

MTF2_KO_ex11_gRNA

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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LMX1B-gRNA-C-del

Plasmid

#131516

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only
LMX1B-gRNA-N-del

Plasmid

#131333

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HoxD del ds1

Plasmid

#131338

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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SKOR1-gRNA-C-del

Plasmid

#131332

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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SKOR1-gRNA-N-del

Plasmid

#131331

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HoxD del control ds

Plasmid

#131336

Purpose

gRNA to delete control region near HoxD. Use with HoxD del control us

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

CTRL-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
HoxD del us1

Plasmid

#131337

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HoxD del control us

Plasmid

#131335

Purpose

gRNA to delete control region near HoxD. Use with HoxD del control ds

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

CTRL-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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EED-KO-gRNA-1

Plasmid

#131321

Purpose

gRNA to knockout EED. Use with EED-KO-gRNA-2

Depositor

Danny Reinberg

Article

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EED-KO-gRNA-1

Use

CRISPR

Expression

Mammalian

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128345

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb4 (Chrnb4 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Sph ELL2

Plasmid

#127270

Purpose

For in vitro translation of human WT ELL2 with internal HA tag

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

WT Mets and HA tag at SphI site (8th Met)

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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ELL2mRNA+polyA

Plasmid

#127256

Purpose

For in vitro translation of human ELL2 with 30 nt pA tail

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

shortens 3'UTR to 40 bp adds A30 tail

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Xba ELL2

Plasmid

#127268

Purpose

For in vitro translation of WT ELL2 with HA tag inserted near N-term

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

ELL2mRNA+polyA with HA tag inserted at aa12 betwe…

Promoter

T7

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only
gh114

Plasmid

#106784

Purpose

expression of gRNA targeting B4GALNT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B4GALNT2 (B4GALNT2 Human)

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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