We narrowed to 10,356 results for: plasmids 101

1. pDONR223_EYS_p.R587I

   Plasmid

   #81485

   Purpose

   Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   EYS (EYS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   R587I

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223_EYS_p.C554S

   Plasmid

   #81491

   Purpose

   Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   EYS (EYS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   C554S

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR223_EYS_p.Q172L

   Plasmid

   #81448

   Purpose

   Gateway Donor vector containing EYS , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Jesse Boehm, William Hahn

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   EYS (EYS Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Q172L

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223_CCND1_p.E172K

   Plasmid

   #82733

   Purpose

   Gateway Donor vector containing CCND1, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   E172K

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDONR223_SERPINB5_p.I159S

   Plasmid

   #82734

   Purpose

   Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   SERPINB5 (SERPINB5 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   I159S; S176P; p.190_192delTDTins25amino acids; p.…

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223_SERPINB5_p.D141A

   Plasmid

   #82735

   Purpose

   Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   SERPINB5 (SERPINB5 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   p. D141A; p. 176S>P; p.190_192delTDTins25amino…

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223_SERPINB5_p.A7T

   Plasmid

   #82736

   Purpose

   Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   SERPINB5 (SERPINB5 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   p. A7T; p.176S>P; p. 190_192delTDTins25; p.194…

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_SERPINB5_p.A42F

   Plasmid

   #82738

   Purpose

   Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   SERPINB5 (SERPINB5 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   p.A42F; p.176S>P; p.190_192delTDTins25; p.194_…

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR223_SERPINB5_p.A165T

   Plasmid

   #82741

   Purpose

   Gateway Donor vector containing SERPINB5, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   SERPINB5 (SERPINB5 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   p. A165T; p.176S>P; p.190_192delTDTins25; p.19…

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFUW-VenusFlag-hCD44

    Plasmid

    #211824

    Purpose

    Express CD44 in mammalian cells

    Depositor

    Haian Fu

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    CD44 (CD44 Human)
    

    Use

    Lentiviral

    Tags

    N-Terminal Venus and Flag tags

    Expression

    Mammalian

    Mutation

    isoform corresponds to UniProt ID P16070-1 with a…

    Promoter

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL4.22-VEGF-HRE::dLUC

    Plasmid

    #128096

    Purpose

    Hypoxia response element real-time luciferase reporter

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Hypoxia response element (x5) from VEGF promoter (VEGFA Human)
    

    Use

    Luciferase

    Tags

    drives expression of destabilized luciferase (2CP…

    Expression

    Mammalian

    Mutation

    Promoter

    inserted VEGF hypoxia response elements (HRE) (x5)

    Available Since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV uN2CpolyGly op GFP (NIID)

    Plasmid

    #224356

    Purpose

    Express GFP tagged upsteram ORF of NOTCH2NLC with an expansion (100x) of GGC repeats with codon optimization (NOT pure GGC repeats)

    Depositor

    Nicolas Charlet-Berguerand

    Article

    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

    Insert

    uN2C with a GGC repeat expansion (100x)
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Codon-optimized for expression in human, so no pu…

    Promoter

    CMV + chimeric intron (CAG)

    Available Since

    Feb. 5, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Tet-O-FUW-EGR1-P2A-mCherry

    Plasmid

    #203856

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR1 (EGR1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBFC1207

    Plasmid

    #186694

    Purpose

    VcDART under control of Lac promoter, Vc_2xBsaI_NT gRNA, GmR+sfGFP cargo, with ET-Seq (AsiSI+SbfI) restriction sites flanking Tn7 Right End

    Depositor

    Jennifer Doudna

    Article

    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

    Inserts

    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_2xBsaI_NT (Guide Stuffer) crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    SbfI+AsiSI dual restriction site for donor plasmid removal during ET-Seq
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLac

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP

    Plasmid

    #135766

    Purpose

    This plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.

    Depositor

    Nadia Mercader Huber

    Article

    Sanz-Morejon et al Cell Rep. 2019 Jul 30;28(5):1296-1306.e6

    Insert

    Wilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
    

    Use

    Zebrafish

    Tags

    Expression

    Mutation

    Promoter

    UAS

    Available Since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-GSDMD_1-270

    Plasmid

    #218229

    Purpose

    Gasdermin1-270 is a pore-forming GSDMD formed by SARS-CoV-2 3CLpro cleavage. Transfection in HEK293 cells showed GSDMD 1-270 pores secrete 3CLpro, nucleocapsid, and in excess, kill cells by pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 1-270

    Promoter

    Available Since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA3.1-GSDMD_1-275

    Plasmid

    #218230

    Purpose

    Gasdermin-D_1-275 is a pore-forming GSDMD formed by cleavage by host caspases. Transfection in HEK293 cells showed GSDMD 1-275 pores secrete 3CLpro, nucleocapsid; in excess, kills cells by pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 1-275

    Promoter

    Available Since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pcDNA3.1-GSDMD_29-484

    Plasmid

    #218231

    Purpose

    Gasdermin-D_29-484 is a non-functional GSDMD formed by SARS-CoV-2 3CLpro cleavage in the N-terminal domain. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 29-484

    Promoter

    Available Since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA3.1-GSDMD_29-270

    Plasmid

    #218232

    Purpose

    Gasdermin-D_29-270 is a non-functional GSDMD formed by two SARS-CoV-2 3CLpro cleavages. Transfection in HEK293 cells showed no pore-forming activity or induction of pyroptosis

    Depositor

    Christopher Overall

    Article

    Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

    Insert

    GSDMD (GSDMD Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    construct only codes for amino acids 29-270

    Promoter

    Available Since

    July 1, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pLCKO-CMV-ACE2-P2A-Blasticidin

    Plasmid

    #237476

    Purpose

    Encodes full length human ACE2 protein, along with blasticidin selection marker.

    Depositor

    Dirk Daelemans

    Article

    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Lentiviral

    Tags

    P2A-Blasticidin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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