We narrowed to 9,888 results for: EPO

1. pcs2-MTS-cox8A-GFP-IRES-mCherry

   Plasmid

   #226103

   Purpose

   Stability reporter construct of the COX8A mitochondrial targeting sequence (MTS) for transient expression in mammalian cells

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Insert

   COX8A (COX8A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   encodes only for mitochondrial targeting sequence…

   Promoter

   Available Since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST-CMV mCherry-GFP-LC3B WT

   Plasmid

   #123230

   Purpose

   Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Use

   Tags

   EGFP and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCAR_sgRNA_puro-mKate-lox2272

   Plasmid

   #162076

   Purpose

   3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-N-Flag-Caspase-1

   Plasmid

   #75128

   Purpose

   Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   caspase 1 (Casp1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   P341S in Caspase-1 (please see depositor comments…

   Promoter

   CMV

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET iGluSnFR3 v857

   Plasmid

   #175186

   Purpose

   Fluorescent reporter for glutamate, third generation, variant 857 soluble form to express in bacteria.

   Depositor

   Kaspar Podgorski

   Article

   Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

   Insert

   iGluSnFR3 v857
   

   Use

   Synthetic Biology

   Tags

   His tag, T7 leader, Xpress epitope

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct

   Plasmid

   #176836

   Purpose

   Targeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   dCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   See Depositor Comments Below

   Promoter

   Tet operator (tetracycline inducible expression)

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV.CBA.eGFP.2A.wtTau

   Plasmid

   #140424

   Purpose

   AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

   Depositor

   Bradley Hyman

   Article

   Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

   Insert

   eGFP-2A-Tau (MAPT Human, Synthetic)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   CBA

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRaGE Pyl TAG GFP Y35TAG

   Plasmid

   #160041

   Purpose

   Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.

   Depositor

   Lital Alfonta

   Article

   Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5

   Inserts

   Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
   Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
   deGFP
   

   Use

   Synthetic Biology

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed tyrosine 35 to TAG stop-codon (Site numbe…

   Promoter

   P70b promoter and T500 terminator, Vibrio natrieg…

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-hygro-STING V155M

   Plasmid

   #102599

   Purpose

   Express STING V155M for IFN-beta Luciferase reporter assay.

   Depositor

   Nicolas Manel

   Article

   Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

   Insert

   STING (STING1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   V155M

   Promoter

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1

    Plasmid

    #216526

    Purpose

    Encodes SARS-CoV-2 spike for JN.1 pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiPGK Puro DEST p38KTRClover

    Plasmid

    #59152

    Purpose

    Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-hygro-STING HAQ

    Plasmid

    #102600

    Purpose

    Express STING HAQ for IFN-beta Luciferase reporter assay.

    Depositor

    Nicolas Manel

    Article

    Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

    Insert

    STING (STING1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    R71H-G230A-R293Q (HAQ)

    Promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pErCas12a EZ Clone

    Plasmid

    #132641

    Purpose

    Expresses ErCas12a and an sgRNA to target a region of interest

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    AAV and CRISPR

    Tags

    3x HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q926R (Please see depositor comments) and BsaI si…

    Promoter

    CMV Promoter

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSG5 EDD C2768A

    Plasmid

    #37192

    Depositor

    Charles Watts, Darren Saunders

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD C2768A (UBR5 Human)
    

    Use

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    Changed Cysteine 2768 to Alanine; K503R (please s…

    Promoter

    SV40

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. p1303 DV-SFTPC-tdTomato

    Plasmid

    #126696

    Purpose

    donor vector for targeting a tdTomato reporter to the human SFTPC locus at the endogenous ATG start site

    Depositor

    Darrell Kotton

    Article

    Jacob et al Cell Stem Cell. 2017 Oct 5;21(4):472-488

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV-Sox10MCS5-GFP-EF1a-mCherry

    Plasmid

    #115782

    Purpose

    Sox10-MCS5-GFP and a constitutive EF1α-mCherry reporter plasmid. Construct generates constitutive red fluorescence and basal promoter cfos conjugated SOXMCS5 enhancer driven GFP expression in a cell.

    Depositor

    Fraser Sim

    Article

    Pol et al Exp Neurol. 2013 Sep;247:694-702. doi: 1

    Inserts

    mouse derived SOX10 Multiple Species Conserved enhancer element 5 conjugated with cfos basal promoter
    EF1alpha promoter driven constitutive mCherry
    

    Use

    Lentiviral

    Tags

    EF1α promoter fused to mCherry and cfos promoter …

    Expression

    Mutation

    Promoter

    SOX10 Multiple Species Conserved enhancer element…

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEMS2241

    Plasmid

    #111896

    Purpose

    ssAAV genome with Ple320 (POU4F1 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple320 (POU4F1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple320 (POU4F1 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFOXA2-T2A-mTagBFP2-PGK-Neo

    Plasmid

    #223191

    Purpose

    BFP reporter plasmid targeting the human FOXA2 gene

    Depositor

    Matthew Birket

    Article

    Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

    Insert

    FOXA2-T2A-mTagBFP2-PGK-Neo-FOXA2 (FOXA2 Human)
    

    Use

    Cre/Lox; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-GAL4-UAS-activemTGFb1-IRES-mCherry-PGK-BFP

    Plasmid

    #223213

    Purpose

    Lentiviral vector - mCherry reporter and TGFb1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    TGFb1 active - membrane (TGFB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMS2273

    Plasmid

    #111899

    Purpose

    ssAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPRE

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    Ple343 (TUBB3 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Ple343 (TUBB3 MiniPromoter)

    Available Since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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