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pimEJ5GFP

Plasmid

#44026

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ5GFP egfp-based chromosomal break reporter

Expression

Mammalian

Mutation

pCAGGS promoter and eGFP separated by pgkPURO cas…

Promoter

pCAGGS

Available Since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDestTol2-QUAS:GCaMP7a

Plasmid

#184817

Purpose

Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

Depositor

Marnie Halpern

Article

Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

Insert

QUAS:GCaMP7a-pA

Use

Zebrafish tol2 transgenesis

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_LamB1

Plasmid

#175528

Purpose

Expression of HaloTag7 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-LamB1

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_NES_HaloTag7

Plasmid

#175527

Purpose

Strict cytosolic expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

NES-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_HaloTag7

Plasmid

#175535

Purpose

Expression of HaloTag7 at the actin filaments of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1_HaloTag7

Plasmid

#175531

Purpose

Lyzosomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_SKL

Plasmid

#175532

Purpose

Peroxysomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-SKL

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pKM464 (deletions)

Plasmid

#108322

Purpose

Orbit integrating plasmid to delete target gene.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integration plasmid for creating knockouts …

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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XLone-Cre

Plasmid

#235216

Purpose

Tet-On 3G-inducible PiggyBac system for expression of Cre in human pluripotent stem cells.

Depositor

Xiaojun Lian

Article

Howells et al Biotechnol Bioeng. 2025 Aug 20. doi: 10.

Insert

Cre

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET29-neoLux1.2

Plasmid

#229952

Purpose

Bacterial expression for de novo luciferase, neoLux1.2

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

neoLux1.2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLumi-LgBiT

Plasmid

#188319

Purpose

Expression of LgBiT via sigma 70 promoter in E. coli

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

LgBiT

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Promoter

sigma 70 promoter

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCFD3-NS

Plasmid

#149545

Purpose

Expression of single pegRNA under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Type

Empty backbone

Use

CRISPR

Expression

Insect

Promoter

U6

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKM496 (deletions) Zeo

Plasmid

#109301

Purpose

ORBIT integrating plasmid for deletion of target gene, using zeocin as a selection marker.

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integrating plasmid

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBsaBM3

Plasmid

#85102

Purpose

P450 BM3 variant (A74G/C773S/C810S)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

P450 BM3

Use

Cloning vector

Mutation

A74G/C773S/C810S

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_tBFP_PGK_mCherry

Plasmid

#79130

Purpose

Lentiviral vector - tBFP reporter for Gal4DBDVP64 synNotch receptors with a constitutive mCherry

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Insert

tBFP

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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paavCAG-post-mGRASP-2A-dTomato

Plasmid

#34912

Purpose

post

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Has Service

AAV5

Insert

postsynaptic mGRASP

Use

AAV

Promoter

CAG

Available Since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-EGFP

Plasmid

#122842

Purpose

N-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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cyto-iATPSnFR1.0

Plasmid

#102550

Purpose

cytoplasmic ATP sensor for mammalian cells

Depositor

Baljit Khakh

Article

Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

Insert

iATPSnFR1.0

Expression

Mammalian

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pDisplay-mSA-EGFP-TM

Plasmid

#39863

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin, EGFP, TM helix

Tags

EGFP, FLAG, and HA

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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