We narrowed to 19,281 results for: Cre

1. pBA439

   Plasmid

   #85967

   Purpose

   Perturb-seq vector backbone

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sgGFP-NT2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. MRGPRD-DuET

   Plasmid

   #213340

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   MRGPRD (MRGPRD Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP TMEM41B-3xFLAG

   Plasmid

   #117415

   Purpose

   Expresses TMEM41B tagged with 3xFLAG in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   TMEM41B (TMEM41B Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. LentiCRISPRv2puro-Ptgs2_B6

   Plasmid

   #170300

   Purpose

   A knock-out vector for the mouse Ptgs2 in C57BL6

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse tyrosinase gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-Myc-Parkin

   Plasmid

   #17612

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiCRISPRv2hyg-Ptgs1

   Plasmid

   #170299

   Purpose

   A knock-out vector for the mouse Ptgs1

   Depositor

   Michiyuki Matsuda

   Article

   Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

   Insert

   A gRNA targeting the mouse Ptgs1 gene.
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-STAT2

   Plasmid

   #71451

   Purpose

   3rd generation lentiviral expression of STAT2

   Depositor

   George Stark

   Article

   Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

   Insert

   STAT2 (STAT2 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. NPY1R-DuET

   Plasmid

   #213354

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   NPY1R (NPY1R Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Str-KDEL_TNF-SBP-mCherry

   Plasmid

   #65279

   Purpose

   synchronize trafficking of TNF from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Str-KDEL_TNF-SBP-EGFP

    Plasmid

    #65278

    Purpose

    synchronize trafficking of TNF from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PEmax_2star_IVT

    Plasmid

    #227674

    Purpose

    plasmid for PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    PEmax_2_star
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-AncBE4max

    Plasmid

    #174164

    Purpose

    This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.

    Depositor

    Wensheng Wei

    Article

    Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

    Insert

    AncBE4max
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

    Plasmid

    #176704

    Purpose

    neural activity dependent release of sbGLuc from presynaptic vesicles

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

    Inserts

    Gaussia luciferase, slow burn
    dTomato
    

    Use

    AAV

    Tags

    P2A and hPOMC1-26

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWF079_LVt-EF1a-TCRGibson_humanTRAC

    Plasmid

    #233448

    Purpose

    Gibson assembly of TCRB-P2A-TCRa

    Depositor

    William Freed-Pastor

    Article

    Ely et al Science. 2025. May 8; 388(6747)

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-REPORT(PGKCMV)-nucTomato-hygro

    Plasmid

    #208573

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    mnucTomato
    d2nucEGFP
    HygroR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK/CMV and minP

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. p23-NLS-RfxCas13d-mcherry-NLS-Flag

    Plasmid

    #165077

    Purpose

    overexpression of RfxCas13d in human cells

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    RfxCas13d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a_MYCN_P2A_Hygro_Barcode

    Plasmid

    #120463

    Purpose

    Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYCN (MYCN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. NPY-td-Orange2

    Plasmid

    #83497

    Purpose

    Expression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.

    Depositor

    Sebastian Barg

    Article

    Gandasi et al PLoS One. 2015 Jun 19;10(6):e0127801. do

    Insert

    NPY (NPY Human)
    

    Use

    Tags

    tandem mOrange2

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIL6-Nluc-hbb

    Plasmid

    #239843

    Purpose

    Template for in vitro transcription of secreted nanoluciferase, with interleukin 6 signal peptide, flanked by the human haemoglobin beta 5' and 3' UTRs.

    Depositor

    Catherine Jopling

    Article

    Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

    Insert

    Secreted nanoluciferase, with interleukin 6 signal peptide, flanked by human haemoglobin beta 5' and 3' UTRs
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    T7 (included as part of insert)

    Available since

    July 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR221-BNT162b2ΔUTR

    Plasmid

    #171214

    Purpose

    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

    Depositor

    Liang Wei Wang

    Article

    Low et al medRxiv 2021.04.27.21256151

    Insert

    BNT162b2ΔUTR
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Kozak sequence changed from CCCGCCACC to GGCTGCAC…

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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