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Dendra2-Vimentin-7

Plasmid

#57745

Purpose

Localization: Intermediate Filaments, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Vimentin (VIM Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pNIR-FbGFPY58/C-JIP

Plasmid

#184684

Purpose

NIR-Fb-based JNK inhibitor

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbGFPY58/C-JIP

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMyosin-miRFP670nano

Plasmid

#127431

Purpose

LC-Myosin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-LC-Myosin

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-mBmi1

Plasmid

#114317

Purpose

TET-inducible expression of the mBmi-1 protein

Depositor

Patrick Salmon

Article

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

mBmi1 (Bmi1 Mouse)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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px330 VRER

Plasmid

#101729

Purpose

Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E, and T1337R mutation…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pJAID5XY

Plasmid

#165051

Purpose

Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

OsTIR1 is codon-optimised for expression in S. ce…

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCDFBB-eGFP

Plasmid

#32550

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

egfp (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pRU292

Plasmid

#167687

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUBQ4-2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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mEos2-Golgi-7

Plasmid

#57382

Purpose

Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Golgi (B4GALT1 Human)

Tags

mEos2

Expression

Mammalian

Mutation

aa 1-82 of NM_001497.3

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV2-hSyn1-NES-ChemoG-CaM

Plasmid

#193817

Purpose

Expression of ChemoG-CaM in primary neurons

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-CaM

Use

AAV

Mutation

EGFP:A206K; HT7: L271E

Promoter

hSyn1

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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5xHRE-MinTK-CRE

Plasmid

#141145

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

5xHRE-MinTK-CRE

Use

Lentiviral

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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Fra-1

Plasmid

#187906

Purpose

Expresses Fra-1 in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

Fra1 (Fosl1 Rat)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET FLIPE intramol-1u

Plasmid

#13549

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol WT (gltI E. coli)

Tags

EYFP

Expression

Bacterial

Mutation

ECFP insert in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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mEos2-CENPA

Plasmid

#57359

Purpose

Localization: Nucleus, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CENPA (CENPA Human)

Tags

mEos2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pFLIPglu-600uDelta13

Plasmid

#12991

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

F16A in binding protein

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT-ChemoL-ATP

Plasmid

#193831

Purpose

CMV overexpression of ChemoL-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoL-ATP

Expression

Mammalian

Mutation

EGFP:A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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Twist-siRNA5

Plasmid

#8456

Depositor

Bob Weinberg

Article

Yang et al Cell 2004 Jun 25;117(7):927-39.

Insert

siTwist (Twist1 Mouse)

Expression

Mammalian

Available Since

May 4, 2005

Availability

Academic Institutions and Nonprofits only
PKAcs-GGS4X-GFP

Plasmid

#108583

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC flag-mRAP1 I312R

Plasmid

#73138

Purpose

Expression of Flag-tagged mRAP1 with single point mutatoin I312R

Depositor

Agnel Sfeir

Article

Yeung et al Cell Rep. 2013 Jun 27;3(6):1847-56. doi:

Insert

Rap1 (Terf2ip Mouse)

Tags

Flag

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only
Syn:NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEcs(ENLYFQ/M)-tTA

Plasmid

#135462

Purpose

Neuronal expression of uFLARE TF component

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

NRX-TM-Nav1.6-CIBN-2xMKII-hLOV-TEVcs(ENLYFQ/M)-tTA

Use

AAV

Tags

HA, flag, VP16

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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