We narrowed to 16,586 results for: EMB

1. pYO1125

   Plasmid

   #235742

   Purpose

   Protein expression of GST-tagged PX domain (Cys at 2) in bacteria

   Depositor

   Roger Williams

   Article

   Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

   Insert

   p40 PX domain (NCF4 Human)
   

   Use

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   aa 2-149 with Cys at 2

   Promoter

   Available since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNDGG006

   Plasmid

   #231310

   Purpose

   Vector part for BEVA; BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 3 p15A narrow host range origin of replication with oriT, CmR.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNDGG007

   Plasmid

   #231311

   Purpose

   Vector part for BEVA; BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 3 pMB1 narrow broad host range origin of replication with oriT, CmR.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNDGG010

   Plasmid

   #231315

   Purpose

   Vector part for BEVA; BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 4 attB recombinase site, AmpR. Also includes FRT EF module for L1 GG cloning.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNDGG022

   Plasmid

   #231306

   Purpose

   Vector part for BEVA; BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 1 Level 1 BsaI cloning site with alternate CAGA 3' fusion site, without T0, AmpR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNDGG009

   Plasmid

   #231314

   Purpose

   Vector part for BEVA; BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.

   Depositor

   Barney Geddes

   Article

   Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

   Insert

   BEVA2.0 Position 4 FRT recombinase site, AmpR. Also includes attB EF module for L1 GG cloning.
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB-Puro-DEST (JDW 925)

   Plasmid

   #229808

   Purpose

   A puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning system

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGLAST-DEST (JDW 1009)

   Plasmid

   #229822

   Purpose

   A single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGM65

   Plasmid

   #220170

   Purpose

   CRISRPi guide RNA against NACA

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   NACA (sgRNA) (NACA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNDGG002

    Plasmid

    #231308

    Purpose

    Vector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNDGG001

    Plasmid

    #231307

    Purpose

    Vector part for BEVA; BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.

    Depositor

    Barney Geddes

    Article

    Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

    Insert

    BEVA2.0 Position 2 Km/Nm antibiotic resistance via nptII, CmR/KmR/NmR.
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-MCS (JDW 455)

    Plasmid

    #229832

    Purpose

    A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    attL1/L2 flanked MCS
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME-StayGoldc4-Giantin (JDW 1355)

    Plasmid

    #229844

    Purpose

    A gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent protein

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    n2StayGoldc4
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xMS-TR-100-PURO HRD

    Plasmid

    #207582

    Purpose

    homologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locus

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
    

    Use

    Tags

    3xMS2

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-Dyskerin

    Plasmid

    #207577

    Purpose

    N-terminal mCherry-tag using SalI/EcoRI

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    generated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid

    #228724

    Purpose

    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

    Plasmid

    #228732

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D56R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

    Plasmid

    #228735

    Purpose

    Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

    Plasmid

    #228731

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEJS2713-CMV-iNme2-ABE8e-i1-L10

    Plasmid

    #228734

    Purpose

    Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid iNme2 nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

    Promoter

    CMV

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge