We narrowed to 11,407 results for: 110

1. GAL 25Q+ProCFPp303

   Plasmid

   #15574

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt25Q + Pro (HTT Human)
   

   Use

   Tags

   CFP

   Expression

   Yeast

   Mutation

   Proline-rich region present

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

2. pSBL_DK198

   Plasmid

   #156425

   Purpose

   Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid

   Depositor

   Stephen Brohawn

   Article

   Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

   Insert

   SARS-CoV-2 3a protein (aa 42-275)
   

   Use

   Tags

   sfGFP-His

   Expression

   Insect

   Mutation

   aa 42-275

   Promoter

   Polyhedrin

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221-RAP1

   Plasmid

   #84525

   Purpose

   This plasmid can be used as a donor for subcloning into Gateway destination vectors.

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Gateway donor plasmid

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   none

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJG486

   Plasmid

   #91195

   Purpose

   WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nTaCas9_H840A+gUbi1+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL N-Myc-hPOT1_IRES_hygro

   Plasmid

   #166406

   Purpose

   express hPOT1 in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   hPOT1 (POT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUB6_ATR

   Plasmid

   #84016

   Purpose

   ATR minigene to test splicing event

   Depositor

   Robert Bradley

   Article

   Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UB6

   Available since

   Jan. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GAL HA25QDProCFPp303

   Plasmid

   #15578

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q Delta Pro (HTT Human)
   

   Use

   Tags

   CFP and HA

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

8. GAL HA103QDProCFPp303

   Plasmid

   #15579

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 103Q Delta Pro (HTT Human)
   

   Use

   Tags

   CFP and HA

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Promoter

   Available since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

   Plasmid

   #46839

   Purpose

   Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

   Depositor

   Phil Sharp

   Article

   Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

   Insert

   Coral Green Fluorescent Protein (cGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. SMT205

    Plasmid

    #134817

    Purpose

    SMT201 with mutated RBS for DHFR, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_A0701

    Plasmid

    #91019

    Purpose

    Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4-P2A-AtCas9_H840A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    H840A

    Promoter

    35S

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAPi

    Plasmid

    #127548

    Purpose

    Empty control for pGAPi-based RNAi silencing in tandem with APT transcript segment

    Depositor

    Luis Vidali

    Article

    Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

    Insert

    Adenine Phosphorybosyltransferase transcript segment
    

    Use

    RNAi; Empty control

    Tags

    Expression

    Mutation

    Promoter

    Ubiquitin

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MyoUTipGAPi

    Plasmid

    #127549

    Purpose

    Positive RNAi control for pGAPi-based RNAi silencing in tandem with APT transcript segment

    Depositor

    Luis Vidali

    Article

    Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

    Insert

    Myosin XIa+b 5'UTR and Adenine Phosphorybosyltransferase transcript segment
    

    Use

    RNAi; Positive control

    Tags

    Expression

    Mutation

    Promoter

    Ubiquitin

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTC393

    Plasmid

    #91221

    Purpose

    protoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNA targeting tomato ANT1
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTC363

    Plasmid

    #91211

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtU6 promoters)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-HA-mIFITM3-C105A

    Plasmid

    #58392

    Purpose

    Expressed murine IFITM3-C105A with an N-terminal HA tag in mammalian cells

    Depositor

    Jacob Yount, Howard Hang

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Cysteine 105 to Alanine

    Promoter

    CMV IE

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

17. 824 pGEX2T PTEN delta274-342

    Plasmid

    #10739

    Depositor

    William Sellers

    Article

    Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Deletion of amino acids 274-342.

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-myc-mIFITM3

    Plasmid

    #58458

    Purpose

    Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

19. Cenp-B DBD INCENP TSS/AAA mCherry

    Plasmid

    #45234

    Purpose

    Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
    

    Use

    Tags

    CENP-B DBD, DNA binding domain of CENP-B and mChe…

    Expression

    Mammalian

    Mutation

    INCENP C-terminal TSS mutated to AAA

    Promoter

    t7

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. px330-MRPsg4

    Plasmid

    #87192

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 4

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #4 (RMRP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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