We narrowed to 16,006 results for: grna

Showing: 8961 - 8980 of 16006 results

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pSLQ2853-3 pHR: U6-Sasgv2CXCR4-1 CMV-EGFP

Plasmid

#84254

Purpose

Expresses optimized Sa sgCXCR4-1 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Publication

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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gh23

Plasmid

#106696

Purpose

expression of gRNA targeting POMT1, Cas9, and GFP

Depositor

Eric-Paul Bennett

Publication

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

POMT1 (POMT1 Human)

Expression

Mammalian

Available since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B1_M13ps

Plasmid

#231422

Purpose

M13 phagemid that expresses sgRNA-B1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ615.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B1

Use

Synthetic Biology

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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HaloXRCC4 sgRNA

Plasmid

#207606

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of XRCC4.

Depositor

Jens Schmidt

Publication

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

TACTGGGTTCAGAAACAAGG

Expression

Mammalian

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C1_M13ps

Plasmid

#231411

Purpose

M13 phagemid that expresses sgRNA-C1 (= sgRNA A5T from Nielsen et al., 2014) from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ272.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C1

Use

Synthetic Biology

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-2_M13ps

Plasmid

#231412

Purpose

M13 phagemid that expresses sgRNA-2 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ171.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-2

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Publication

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_LacI_pLac-sgRNA-1

Plasmid

#231408

Purpose

sgRNA-1 expression from an IPTG-inducible promoter.

Depositor

Manish Kushwaha

Publication

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
LacI

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_AraC_pBAD-sgRNA-1

Plasmid

#231407

Purpose

sgRNA-1 expression from an arabinose-inducible promoter.

Depositor

Manish Kushwaha

Publication

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

sgRNA-1
AraC

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A1_M13ps

Plasmid

#231414

Purpose

M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-A1

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-1_M13ps

Plasmid

#231409

Purpose

M13 phagemid that expresses sgRNA-1 from a strong constitutive promoter.

Depositor

Manish Kushwaha

Publication

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-1

Use

Synthetic Biology

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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p103DestTol2pA2-4xU6sgRNA

Plasmid

#227778

Purpose

Destination vector with U6 driven 4 sgRNAs

Depositor

Bushra Raj

Publication

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Insert

4xU6:sgRNA

Use

CRISPR

Promoter

U6

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #1

Plasmid

#207105

Purpose

pX330 based plasmid for expression of Cas9 and the CGCTATCAAGATTTATACCT sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CGCTATCAAGATTTATACCT

Expression

Mammalian

Promoter

CMV and U6

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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ATG13 sgRNA

Plasmid

#207558

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG13 locus

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

ggaaactgatctcaattccc

Expression

Mammalian

Promoter

CMV and U6

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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HaloTag KO sgRNA

Plasmid

#207102

Purpose

pX330 based plasmid for expression of Cas9 and the GTCGATGTTGGTCCGCGCGA sgRNA to target the HaloTag coding sequence.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GTCGATGTTGGTCCGCGCGA

Expression

Mammalian

Promoter

CMV and U6

Available since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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SHLD3 KO sgRNA #2

Plasmid

#207106

Purpose

pX330 based plasmid for expression of Cas9 and the CTGAAGGAACAGACTAATTC sgRNA to target the SHLD3 locus..

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

CTGAAGGAACAGACTAATTC

Expression

Mammalian

Promoter

CMV and U6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA5_8xCTS-ECFP-pA

Plasmid

#200247

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 5

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA5_8xCTS

Expression

Mammalian

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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sgRNA3_8xCTS-ECFP-pA

Plasmid

#200245

Purpose

Mammalian transfections; 8xCTS-ECFP reporter construct 3

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

sgRNA3_8xCTS

Expression

Mammalian

Available since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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