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Showing: 1321 - 1350 of 13590 results

  1. pYLCRISPR/Cas9P35S-B

    Plasmid
    #66190
    Purpose
    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection
    Depositor
    Yao-Guang Liu
    Article
    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(
    Insert
    Cas9
    Use
    Tags
    Expression
    Plant
    Mutation
    plant-codon optimized with higher GC contents (62…
    Promoter
    cauliflower mosaic virus 35S promoter (P35S)
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    66190-plasmid-map-sequence-id-169495

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  17. pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract

    Plasmid
    #138525
    Purpose
    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence.
    Depositor
    Richard Jenner
    Article
    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899
    Insert
    Control non-PRC2 binding A-tract repeat RNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138525-plasmid-map-sequence-id-292292

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  20. pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR

    Plasmid
    #118622
    Purpose
    expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons
    Depositor
    Marina Chekulaeva
    Article
    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525
    Insert
    cdc42E7-E6 3'UTR (Cdc42 Mouse)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    changed ttgcttt to cggtaag (496 - 502 nt in 3…
    Promoter
    synapsin I (rat)
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    118622-plasmid-map-sequence-id-230551

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  21. pMEXneo-TrkC-Δ6

    Plasmid
    #190204
    Purpose
    To express the human TrkC that has in the extracellular domain only the second IgG like domain. Still binds NT-3.
    Depositor
    Pantelis Tsoulfas
    Article
    Urfer et al J Biol Chem. 1998 Mar 6;273(10):5829-40.
    Insert
    TrkC (NTRK3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    The extracellualr domain conatins only the signal…
    Promoter
    Moloney mouse sarcoma virus right LTR
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190204-plasmid-map-sequence-id-373714

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  22. pRosettav2

    Plasmid
    #115557
    Purpose
    To determine correct antibiotic selection conditions in mammalian cells; standard for titering experiments
    Depositor
    John Doench
    Insert
    Hygromycin-Blasticidin-Puromycin-EGFP
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    115557-plasmid-map-sequence-id-226246

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  23. pLenti-ePEG-LINE1-loxPsym

    Plasmid
    #216866
    Purpose
    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements
    Depositor
    Leopold Parts
    Insert
    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216866-plasmid-map-sequence-id-441211

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  25. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid
    #195487
    Purpose
    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7
    Use
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    C61T, C262V
    Promoter
    T7
    Available since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195487-plasmid-map-sequence-id-393190

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  30. pPyCAG-Nanog(K140A)-IP

    Plasmid
    #62998
    Purpose
    Expresses Mouse Nanog K140A Mutants. It is modified from pPyCAG-Nanog-IP (Addgene Plasmid #13838).
    Depositor
    Shinya Yamanaka
    Insert
    Mouse Nanog K140A Mutant (Nanog Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Lysine 140 (141 AA in Mouse Protein) to A…
    Promoter
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    62998-plasmid-map-sequence-id-104601

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Showing: 1321 - 1350 of 13590 results