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Showing: 2201 - 2250 of 4184 results

  1. pJK645

    Plasmid
    #71700
    Purpose
    Produces E. coli thioredoxin (EcTrxA)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71700-plasmid-map-sequence-id-131737

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  2. pET-(neg27)GFP-GGS9-Cre-6xHis

    Plasmid
    #89253
    Purpose
    Expresses GFP with -27 theoretical charge fused to Cre in E.Coli
    Depositor
    David Liu
    Insert
    (neg27)GFP-GGS9-Cre-6xHis
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89253-plasmid-map-sequence-id-176140

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  3. pJK266

    Plasmid
    #73326
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioD with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioD
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73326-plasmid-map-sequence-id-135034

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  4. pJK409

    Plasmid
    #72423
    Purpose
    Produces Acetobacter aceti 1023 Est14, C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    72423-plasmid-map-sequence-id-135791

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  5. pJK405

    Plasmid
    #72418
    Purpose
    Produces Acetobacter aceti 1023 Est13, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72418-plasmid-map-sequence-id-135782

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  6. pJK403

    Plasmid
    #72416
    Purpose
    Produces Acetobacter aceti 1023 Est07, N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    arylesterase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    72416-plasmid-map-sequence-id-135537

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  7. pJK690

    Plasmid
    #72380
    Purpose
    Produces Escherichia coli monothiol glutaredoxin GrxD, N-terminal His6 tag (H6EcGrxD)
    Depositor
    T. J. Kappock
    Insert
    glutaredoxin-4
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72380-plasmid-map-sequence-id-134002

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  8. MAC045-C12

    Plasmid
    #58753
    Purpose
    bacterial expression of PDB 4NXJ
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4NXJ
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Aug. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. Py-ADA

    Plasmid
    #25576
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Py-ADA
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pJK365

    Plasmid
    #72404
    Purpose
    Produces Acetobacter aceti 1023 Est01
    Depositor
    T. J. Kappock
    Insert
    acyl-CoA thioester hydrolase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    72404-plasmid-map-sequence-id-134631

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  11. VEEV_KP282671.1_NSP2_Pro_NHisTEV_CThromAvi

    Plasmid
    #234384
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp2_VEEV
    Use
    Tags
    LVPRGSSSGLNDIFEAQKIEWHE and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234384-plasmid-map-sequence-id-470056

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  12. VEEV_KP282671.1_NSP2_FL_NHisTEV_CThromAvi

    Plasmid
    #234383
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp2_VEEV
    Use
    Tags
    LVPRGSSSGLNDIFEAQKIEWHE and MHHHHHHSSGRENLYFQG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    234383-plasmid-map-sequence-id-469873

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  13. pAIP4_1YW5

    Plasmid
    #234235
    Purpose
    Heterologous protein expression of peptidyl-prolyl cis-trans isomerase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1YW5 (ESS1 Candida albicans)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234235-plasmid-map-sequence-id-464199

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  14. pAIP4_1TJE

    Plasmid
    #234203
    Purpose
    Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TJE
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234203-plasmid-map-sequence-id-464168

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  15. pAIP4_5H28

    Plasmid
    #234184
    Purpose
    Heterologous protein expression of oxysterol-binding protein homolog 1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5H28 (SWH1 Budding Yeast)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234184-plasmid-map-sequence-id-464149

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  16. pAIP4_1HKA

    Plasmid
    #234169
    Purpose
    Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1HKA (folK Escherichia coli)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234169-plasmid-map-sequence-id-464135

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  17. pAIP4_4RZ9

    Plasmid
    #234164
    Purpose
    Heterologous protein expression of pre-mRNA-splicing factor 38A in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4RZ9 (PRPF38A Human)
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234164-plasmid-map-sequence-id-464130

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  18. pAIP4_5IHW

    Plasmid
    #234148
    Purpose
    Heterologous protein expression of serine-aspartate repeat-containing protein E in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5IHW
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234148-plasmid-map-sequence-id-464114

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  19. pAIP4_2OKT

    Plasmid
    #234149
    Purpose
    Heterologous protein expression of o-succinylbenzoate synthase (OSB synthase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2OKT
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234149-plasmid-map-sequence-id-464115

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  20. pNYCOMPS-C-Lic-His-FLAG

    Plasmid
    #182505
    Purpose
    pET based vector for protein expression in E.coli for targets fused with C-terminal Flag and His
    Depositor
    Renato Bruni
    Type
    Empty backbone
    Use
    Tags
    TEV-FLAG-10x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182505-plasmid-map-sequence-id-361391

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  21. 5VS7 (PP-BRD4)

    Plasmid
    #101264
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PP-BRD4
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 3, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    101264-plasmid-map-sequence-id-193497

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  22. pJK241

    Plasmid
    #71693
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp404>Phe mutant (AarAH6-W404F)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Tro404 to Phe
    Promoter
    T7 promoter
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71693-plasmid-map-sequence-id-129669

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  23. pJK699

    Plasmid
    #71691
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Asp371>Asn mutant (AarAH6-D371N)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Asp371 to Asn
    Promoter
    T7 promoter
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71691-plasmid-map-sequence-id-129671

    Enlarge

  24. pJK708

    Plasmid
    #71692
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase (AarA) with His6 tag and Trp400>Phe mutant (AarAH6-W400F)
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    changed Trp400 to Phe
    Promoter
    T7 promoter
    Available since
    June 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    71692-plasmid-map-sequence-id-129670

    Enlarge

  25. pJK195

    Plasmid
    #73321
    Purpose
    Produces Janthinobacterium lividum violacein biosynthesis protein VioC with a N-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    violacein biosynthesis protein VioC
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    contains silent mutation in Val428
    Promoter
    T7
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73321-plasmid-map-sequence-id-135029

    Enlarge

  26. pJK536

    Plasmid
    #72444
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein adjacent to purEK genes (AaOrfX)
    Depositor
    T. J. Kappock
    Insert
    hypothetical protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    synthetic gene with ~40 silent substitutions
    Promoter
    T7
    Available since
    Feb. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    72444-plasmid-map-sequence-id-136729

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  27. pJK539

    Plasmid
    #72515
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase large subunit (AaPurL)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72515-plasmid-map-sequence-id-132543

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  28. pJK555

    Plasmid
    #72521
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ)
    Depositor
    T. J. Kappock
    Insert
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    72521-plasmid-map-sequence-id-132606

    Enlarge

  29. 2QQ8

    Plasmid
    #60110
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TBC1D14:TBC1D14_dml-X01:C201000
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. 4JUY

    Plasmid
    #60083
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RNF31:YTC010-F07:C227920
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  31. MAC04E-A09

    Plasmid
    #58757
    Purpose
    bacterial expression of PDB 4O2Z
    Depositor
    Cheryl Arrowsmith
    Insert
    PDB:4O2Z
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available since
    Sept. 19, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. 2Q3F

    Plasmid
    #51356
    Depositor
    Cheryl Arrowsmith
    Insert
    2Q3F (RRAGD Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  33. Pb-OMPDC

    Plasmid
    #25573
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pb-OMPDC
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. Pv-ASL

    Plasmid
    #25587
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    Pv-ASL
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. pTCF236 HLA-B*40:01

    Plasmid
    #180463
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*40:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180463-plasmid-map-sequence-id-355082

    Enlarge

  36. pTCF7 HLA-B*07:02

    Plasmid
    #180446
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*07:02
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180446-plasmid-map-sequence-id-355789

    Enlarge

  37. pJA48 HLA-B*57:01

    Plasmid
    #180444
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*57:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    180444-plasmid-map-sequence-id-366471

    Enlarge

  38. pTCF5 HLA-A*01:01

    Plasmid
    #180448
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*01:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180448-plasmid-map-sequence-id-355109

    Enlarge

  39. pTCF37 HLA-B*35:01

    Plasmid
    #180450
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*35:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    180450-plasmid-map-sequence-id-358091

    Enlarge

  40. pTCF158 Human beta-2 microglobulin

    Plasmid
    #180457
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Human beta-2 microglobulin
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180457-plasmid-map-sequence-id-355801

    Enlarge

  41. pTCF156 HLA-A*02:01

    Plasmid
    #180456
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*02:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180456-plasmid-map-sequence-id-355799

    Enlarge

  42. 4X3G

    Plasmid
    #64663
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIAH1 (SIAH1 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    April 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  43. pTCF6 HLA-A*24:02

    Plasmid
    #180445
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*24:02
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180445-plasmid-map-sequence-id-355843

    Enlarge

  44. pTCF48 HLA-A*11:01

    Plasmid
    #180449
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-A*11:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    Feb. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    180449-plasmid-map-sequence-id-355113

    Enlarge

  45. pTCF10 HLA-B*08:01

    Plasmid
    #180447
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*08:01
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Promoter
    Available since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180447-plasmid-map-sequence-id-355840

    Enlarge

  46. pTCF200 Mouse beta-2 microglobulin

    Plasmid
    #180462
    Purpose
    E. coli expression of class I MHC light chain
    Depositor
    John Altman
    Insert
    Mouse beta-2 microglobulin
    Use
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    180462-plasmid-map-sequence-id-355083

    Enlarge

  47. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Use
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    67921-plasmid-map-sequence-id-197668

    Enlarge

  48. RAB25

    Plasmid
    #26163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB25:HPC038-B06:C25773 (RAB25 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  49. RAB27B

    Plasmid
    #25545
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB27B (RAB27B Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  50. RAB31

    Plasmid
    #25552
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB31 (RAB31 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
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