We narrowed to 6,273 results for: nls
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Plasmid#169851PurposeMammalian Expression, sauCas9 based prime editorDepositorInsertSaCas9-N580A-M-MLV
TagsbpSV40 NLS and SV40 NLS and cmyc-NLS and bpSV40 N…ExpressionMammalianPromotercmvAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eBE-S3
Plasmid#101093PurposeExpresses eBE-S3 in mammalian cellsDepositorInserteBE-S3
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-SacB-Cas9-T2A-mCherry_(BbsI)
Plasmid#117070PurposeEncodes Cas9, T2A-mCherry, and AmpR which creates a CRISPR system application with reliable positive selectionDepositorInsertsCas9
SacB
UseCRISPRTags3x FLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh and U6Available SinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCR158
Plasmid#240639PurposeExpression of NLS-Kaede in Exaiptasia diaphanaDepositorInsertsAIPGENE865 promoter
Kaede
UseExpression of nls-kaede in exaiptasia diaphanaTagsSV40 NLSMutationnaturally occurring 749-bp insertion at bp 628 (f…PromoterExaiptasia diaphana Elongation Factor 1 alpha (AI…Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
BII-tdT-inv-GFP
Plasmid#133393PurposePiggybac vector for bidirectional promoter reporter assayDepositorTypeEmpty backboneExpressionMammalianPromoterNo promoterAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-smURFP
Plasmid#133399PurposePiggybac vector for constitutive expression of smURFP-NLSDepositorInsertsmURFP-NLS
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT22iuasvApoWtEos3N(#486)
Plasmid#184107Purposecyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenicDepositorInsertmyr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
UseZebrafish transgenesis (blue eyes)MutationDIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …Promoter4xUASAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-iRFP670
Plasmid#133397PurposePiggybac vector for constitutive expression of iRFP670-NLSDepositorInsertiRFP-670-NLS
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pY125 (pcDNA3.1-huBfCpf1)
Plasmid#92301PurposeExpression of humanized BfCpf1DepositorInserthuBfCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY115 (pcDNA3.1-huMlCpf1)
Plasmid#92275PurposeExpression of humanized MlCpf1DepositorInserthuMlCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY112 (pcDNA3.1-huSaCpf1)
Plasmid#92269PurposeExpression of humanized SaCpf1DepositorInserthuSaCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-eBE-Y130F
Plasmid#113423PurposeExpresses hA3A-eBE-Y130F in mammalian cellsDepositorInserthA3A-eBE-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianMutationhAPOBEC3A_Y130FPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
SpyCas9 PE2*-3HA
Plasmid#169854PurposeMammalian Expression, SpyCas9 PE2* prime editor with 3HA-tagDepositorInsertSpCas9-H840A-star-M-MLV-3HA
Tags3XHA tag, bpSV40 NLS and SV40 NLS, and cmyc-NLS a…ExpressionMammalianPromotercmvAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-eBE-Y132D
Plasmid#113429PurposeExpresses hA3A-eBE-Y132D in mammalian cellsDepositorInserthA3A-eBE-Y132D (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
UseCRISPRExpressionMammalianMutationhAPOBEC3A_Y132DPromoterCMVAvailable SinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRD439
Plasmid#168465PurposeFor the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mTurquoise2-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD437
Plasmid#168463PurposeFor the insertion pf NLS-mEos3.2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEos3.2-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD438
Plasmid#168464PurposeFor the insertion pf NLS-mEos3.2-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEos3.2-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD440
Plasmid#168466PurposeFor the insertion pf NLS-mCherry-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mCherry-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD441
Plasmid#168467PurposeFor the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mEGFP-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD442
Plasmid#168468PurposeFor the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-eYFP-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRD444
Plasmid#168470PurposeFor the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex systemDepositorInsertNLS-mNeonGreen-H-NSdbd-NLS
ExpressionMammalianPromoterCMV promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pY116 (pcDNA3.1-huMb2Cpf1)
Plasmid#92292PurposeExpression of humanized Mb2Cpf1DepositorInserthuMb2Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY111 (pcDNA3.1-huTsCpf1)
Plasmid#92267PurposeExpression of humanized TsCpf1DepositorInserthuTsCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJuly 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY117 (pcDNA3.1-huMb3Cpf1)
Plasmid#92293PurposeExpression of humanized Mb3Cpf1DepositorInserthuMb3Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
lenti.Cas9.BFP.sgRNA.parental
Plasmid#196715PurposeSortable WT-SpCas9 expression and guide cassette (1-vector system).DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pY113 (pcDNA3.1-huPb2Cpf1)
Plasmid#92272PurposeExpression of humanized Pb2Cpf1DepositorInserthuPb2Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY119 (pcDNA3.1-huLb5Cpf1)
Plasmid#92295PurposeExpression of humanized Lb5Cpf1DepositorInserthuLb5Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY118 (pcDNA3.1-huLb4Cpf1)
Plasmid#92294PurposeExpression of humanized Lb4Cpf1DepositorInserthuLb4Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY124 (pcDNA3.1-huBsCpf1)
Plasmid#92300PurposeExpression of humanized BsCpf1DepositorInserthuBsCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-104
Plasmid#79842PurposeExpresses NLS-PpsR2-VP16 under CMV promoterDepositorInsertNLS-PpsR2-VP16
TagsNuclear localization signal of nuclear cap-bindin…ExpressionMammalianMutationRpPpsR2 cysteine 439 changed to serinePromoterCMVAvailable SinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNB648
Plasmid#60140PurposeNanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.DepositorInsertNLuc
TagsNuclear localization signal (NLS)ExpressionYeastPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB330
Plasmid#60147PurposeDisintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)DepositorInsertNLuc
TagsNuclear localization signal (NLS)ExpressionYeastPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB774
Plasmid#60114PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)DepositorInsertGrLuc
UseUnspecifiedTagsClpXP degron and Nuclear localization signal (NLS…Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB775
Plasmid#60115PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)DepositorInsertYeLuc
UseUnspecifiedTagsClpXP degron and Nuclear localization signal (NLS…Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB776
Plasmid#60117PurposeRed beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)DepositorInsertRdLuc
UseUnspecifiedTagsClpXP degron and Nuclear localization signal (NLS…Available SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCL33-decoder
Plasmid#159451PurposeInsert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.DepositorInsertPfus1mut-tetR-nls-malE-tCyc1
UseSynthetic BiologyExpressionYeastPromoterPfus1mutAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCerulean-Nucleus-12
Plasmid#54556PurposeLocalization: Nucleus, Excitation: 433, Emission: 475DepositorTypeEmpty backboneTagsmCerulean-NLSExpressionMammalianAvailable SinceJuly 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pY130 (pcDNA3.1-U6-huBsCpf1)
Plasmid#92306PurposeExpression of humanized BsCpf1 and crRNADepositorInserthuBsCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY128 (pcDNA3.1-U6-huMb2Cpf1)
Plasmid#92304PurposeExpression of humanized Mb2Cpf1 and crRNADepositorInserthuMb2Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY129 (pcDNA3.1-U6-huMb3Cpf1)
Plasmid#92305PurposeExpression of humanized Mb3Cpf1 and crRNADepositorInserthuMb3Cpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY127 (pcDNA3.1-U6-huTsCpf1)
Plasmid#92303PurposeExpression of humanized TsCpf1 and crRNADepositorInserthuTsCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY120 (pcDNA3.1-huFbCpf1)
Plasmid#92296PurposeExpression of humanized FbCpf1DepositorInserthuFbCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY126 (pcDNA3.1-huBoCpf1)
Plasmid#92302PurposeExpression of humanized BoCpf1DepositorInserthuBoCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY123 (pcDNA3.1-huCMaCpf1)
Plasmid#92299PurposeExpression of humanized CMaCpf1DepositorInserthuCMaCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY114 (pcDNA3.1-huPgCpf1)
Plasmid#92273PurposeExpression of humanized PgCpf1DepositorInserthuPgCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY122 (pcDNA3.1-huCPbCpf1)
Plasmid#92298PurposeExpression of humanized CPbCpf1DepositorInserthuCPbCpf1
TagsNLS and NLS-3xHAExpressionMammalianAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRDA174_EF1a_EnAsCas12a_BFP
Plasmid#196724PurposeSortable and selectable EnAsCas12a.DepositorInsertenASCas12a-HA-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS - HA and SV40 NLSMutationE174R, S542R, K548RPromoterEF1aAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-BnTW
Plasmid#133365PurposePiggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLSDepositorInserttdTomato-NLS
TagsFlag-tagged tdTomatoExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti.Cas9.BFP.Blast
Plasmid#196714PurposeWT SpCas9 with BSD-TagBFP for selection and sortingDepositorInsertCas9-T2A-BSD-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterEF1aAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-EBFP2-nuc
Plasmid#36085DepositorTypeEmpty backboneUseLentiviralTagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…Available SinceJune 1, 2012AvailabilityAcademic Institutions and Nonprofits only