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pYPQ291-D573A-SRDX

Plasmid

#129683

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

BthCas12b-SRDX

Use

CRISPR; Gateway compatible bthcas12b entry clone

Tags

Expression

Plant

Mutation

D573A; BthCas12b is rice codon optimized

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa3.8.3

Plasmid

#136374

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aac.3

Plasmid

#136377

Purpose

Gateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Aa1.2.3

Plasmid

#136372

Purpose

Gateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is used

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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hC73S-HA

Plasmid

#111529

Purpose

Expresses human KCNB1 C73S in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73S-Kv2.1 + HA (KCNB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C73S

Promoter

CMV

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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hC73A-HA

Plasmid

#111526

Purpose

Expresses human KCNB1 C73A in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73A-Kv2.1 + HA (KCNB1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C73A

Promoter

CMV

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pOME0245M

Plasmid

#182607

Purpose

Reverse complement PAN-RNA pOME0244 Human herpesvirus 8 strain JSC-1 clone BAC16, complete genome.

Depositor

Dirk Dittmer

Insert

Reverse compliment for PAN_RNA (pOME0244)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCas9-EGFP

Plasmid

#63592

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Phil Sharp, Feng Zhang

Article

Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

Inserts

Cas9
EGFP

Use

CRISPR and Lentiviral

Tags

FLAG and P2A

Expression

Mammalian

Mutation

Promoter

EFS-NS

Available since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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HER2 WT

Plasmid

#16257

Depositor

Mien-Chie Hung

Article

Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

Insert

HER2 (ERBB2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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CHOP promoter (-649/+136) pmCherry-1

Plasmid

#36035

Depositor

Quan Lu

Article

Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

Insert

CHOP promoter (-647 to +136) (DDIT3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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Pinducer20-BSD-scFv-sfGFP-DNMT3A1

Plasmid

#115780

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Article

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

scFv-sfGFP-DNMT3A1 (DNMT3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2

Plasmid

#52300

Purpose

full length YTHDF2 with N-terminal flag tag for mammalian cell expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

T7

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_Ado1.0med

Plasmid

#153286

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF3

Plasmid

#70088

Purpose

Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-N-HA-Pcdh15_mini_V4

Plasmid

#199185

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

HA-mmPcdh15_mini_V4-CD2

Use

AAV

Tags

Human influenza hemagglutinin (HA)

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF1

Plasmid

#70087

Purpose

Encode YTHDF1 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_Ado1.0med

Plasmid

#153289

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in astrocytes

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Tags

Expression

Mutation

Promoter

GfaABC1D

Available since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-eGFP-PGK-mCherry

Plasmid

#191862

Purpose

Heat inducible eGFP reporter with constitutive mCherry marker

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

eGFP
mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

HSPA7 promoter and PGK promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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