We narrowed to 3,788 results for: golden gate

1. pLPP5

   Plasmid

   #209967

   Purpose

   Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P_sppQ)
   

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. BB3rN_14*

   Plasmid

   #98550

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLP-mScarlet-I

   Plasmid

   #209993

   Purpose

   Contains Level 0 Part: Coding sequences (mScarlet-I) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher, Georg Fritz

   Article

   Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

   Insert

   mScarlet-I
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. BB3aZ_14*

   Plasmid

   #98542

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BB3aN_14*

   Plasmid

   #98548

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. BB1_34_RPS25Att

   Plasmid

   #98526

   Purpose

   BB1 with insert: RPS25Att

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   RPS25Att
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. BB1_23_eGFP

   Plasmid

   #98518

   Purpose

   BB1 with insert: CDS eGFP

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   eGFP
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BB3aK_14*

   Plasmid

   #98529

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BB1_12_pLAT1

   Plasmid

   #98509

   Purpose

   BB1 with insert: promoter PLAT1

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Insert

   LAT1 promoter
   

   Expression

   Bacterial

   Mutation

   Internal BsaI and BpiI sites removed from insert

   Available Since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. BB1_12_pPFK300

    Plasmid

    #98510

    Purpose

    BB1 with insert: promoter PPpPfk

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    PpPfk promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BB1_12_pDAS2

    Plasmid

    #98513

    Purpose

    BB1 with insert: promoter PDAS2

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    DAS2 promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BB1_12_pRPL2A

    Plasmid

    #98508

    Purpose

    BB1 with insert: promoter PRPL2A

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    RPL2A promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. BB1_12_pDAS1

    Plasmid

    #98515

    Purpose

    BB1 with insert: promoter PDAS1

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Insert

    DAS1 promoter
    

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BB3rN_AH

    Plasmid

    #98556

    Purpose

    empty BB3 for insertion and overexpression of 7 genes in P. pastoris

    Depositor

    Diethard Mattanovich, Michael Sauer, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKEK3235

    Plasmid

    #234107

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKEK3220

    Plasmid

    #234099

    Purpose

    CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

    Depositor

    Karl Klose

    Article

    Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

    Insert

    sfGFP
    

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    S30R/Y39N/N105T/Y145F/I171V/A206V

    Promoter

    pJ23100-BsaI

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pp-CBBC-Fluc

    Plasmid

    #232920

    Purpose

    Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.

    Depositor

    Philippe Mangeot

    Article

    Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLPC53

    Plasmid

    #209962

    Purpose

    Contains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    5' Connector (5C1CSN_Tcat+sai)
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pDM097

    Plasmid

    #216819

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold
    

    Tags

    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGA-pTRE-B

    Plasmid

    #218894

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    TRE
    

    Use

    Synthetic Biology

    Mutation

    580G>C

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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