Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 927 results for: pgem

Clear keywords
Showing: 881 - 900 of 927 results

  1. pVD1 Multiplexing Edit E3-E4-En-1 (GB2244)

    Plasmid
    #160566
    Purpose
    GBoligomers for the position [3_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Multiplexing Edit (E3-E4-En-1)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160566-plasmid-map-sequence-id-319680

    Enlarge

  2. pVD1 Multiplexing Edit En-1 (GB2242)

    Plasmid
    #160564
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Multiplexing Edit (En-1)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160564-plasmid-map-sequence-id-319675

    Enlarge

  3. pVD1 Multiplexing Edit E4 (GB2241)

    Plasmid
    #160563
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA.
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Multiplexing Edit (E4)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160563-plasmid-map-sequence-id-319674

    Enlarge

  7. pVD1 tRNA-gRNA position E3 (GB2240)

    Plasmid
    #160562
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the third position (positon [D3_4]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tRNA-gRNA position E3 (Multiplexing Edit)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160562-plasmid-map-sequence-id-319301

    Enlarge

  8. pD10AH840AhCas9 (GB1041)

    Plasmid
    #68207
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part
    Depositor
    Diego Orzaez
    Publication
    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed; human codon optimis…
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    68207-plasmid-map-sequence-id-135575

    Enlarge

  9. pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

    Plasmid
    #160568
    Purpose
    tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Publication
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    M1_1-pTRNA-scf 2.1
    Use
    CRISPR
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160568-plasmid-map-sequence-id-319683

    Enlarge

  10. pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

    Plasmid
    #160567
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)
    Use
    CRISPR and Synthetic Biology
    Mutation
    BsaI and BsmBI sites removed
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160567-plasmid-map-sequence-id-319681

    Enlarge

  11. x.Amylase

    Plasmid
    #87132
    Purpose
    To make in situ hybridization probe for Xenopus amylase.
    Depositor
    Francesca Spagnoli
    Insert
    Amylase
    Expression
    Mammalian
    Available since
    March 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  12. XE50 XPKC-beta

    Plasmid
    #16747
    Depositor
    Randall Moon
    Insert
    XPKC-Beta
    Use
    Xenopus expression
    Available since
    March 14, 2008
    Availability
    Academic Institutions and Nonprofits only
Showing: 881 - 900 of 927 results