We narrowed to 920 results for: pgem

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pVD1 M1_3-pTRNA-scf 2.1 (GB2073)

Plasmid

#160558

Purpose

tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

Depositor

Diego Orzaez

Publication

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

M1_3-pTRNA-scf 2.1

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

Plasmid

#160567

Purpose

tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA

Depositor

Diego Orzaez

Publication

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pVD1 M2_3-pTRNA-scf 2.1 (GB2074)

Plasmid

#160559

Purpose

tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

Depositor

Diego Orzaez

Publication

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

M2_3-pTRNA-scf 2.1

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Available since

Feb. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pD10AH840AhCas9 (GB1041)

Plasmid

#68207

Purpose

Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed; human codon optimis…

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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x.Amylase

Plasmid

#87132

Purpose

To make in situ hybridization probe for Xenopus amylase.

Depositor

Francesca Spagnoli

Insert

Amylase

Expression

Mammalian

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only
pAW32

Plasmid

#110225

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt1 promoter

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW33

Plasmid

#110226

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt41 promoter

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAW34

Plasmid

#110227

Purpose

integration of loxP site directly upstream of gene start site - contains S. pombe nmt81 promoter

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Type

Empty backbone

Use

Cre/Lox

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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BtA2

Plasmid

#238982

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Jeffrey Benovic, Vsevolod Gurevich

Publication

Sterne-Marr et al J Biol Chem. 1993 Jul 25;268(21):15640-8

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

none

Promoter

SP6

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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XE50 XPKC-beta

Plasmid

#16747

Depositor

Randall Moon

Insert

XPKC-Beta

Use

Xenopus expression

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
BtA2-3A

Plasmid

#238984

Purpose

In vitro transcription of mutant bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Publication

Celver et al J Biol Chem. 2002 Mar 15;277(11):9043-8.

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

I386A,V387A,F388A

Promoter

SP6

Available since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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BtA1N

Plasmid

#238979

Purpose

In vitro transcription of bovine arrestin-1

Depositor

Vsevolod Gurevich

Publication

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-1 (SAG Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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BtA2N

Plasmid

#238980

Purpose

In vitro transcription of bovine arrestin-2 long isoform

Depositor

Vsevolod Gurevich

Publication

Vishnivetskiy et al J Biol Chem. 2004 Jan 9;279(2):1262-8. d

Insert

arrestin-2 long (ARRB2 Bovine)

Use

In vitro transcription

Mutation

silent

Promoter

SP6

Available since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pUPD DsRed-CT (GB1183)

Plasmid

#160556

Purpose

DsRed for C-terminal fusions

Depositor

Diego Orzaez

Insert

DsRed_CT

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pAW41

Plasmid

#110228

Purpose

integration of loxP site a distance upstream of gene start site

Depositor

Tony Carr

Publication

Watson et al Gene. 2008 Jan 15;407(1-2):63-74

Insert

ura4+ (ura4 Fission Yeast)

Use

Cre/Lox

Mutation

See depositor comments below.

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD Pnos (GB0555)

Plasmid

#160555

Purpose

Nos promoter for N-terminal fusions

Depositor

Diego Orzaez

Insert

pNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-RFP

Plasmid

#255759

Purpose

General tracer plasmid. RFP expressed under the ubiquitous CAG promoter

Depositor

Natalya Nikitina

Publication

Oberholzer et al Dev Biol. 2026 Jun;534:160-172. doi: 10.

Insert

CAG promoter expressing human H2B linked to mRFP1 reporter protein

Use

Reporter

Tags

mRFP1

Expression

Mammalian

Mutation

N/A

Available since

Aug. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pNH (GB0064)

Plasmid

#68165

Purpose

Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlNH promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPMtb (GB0080)

Plasmid

#68167

Purpose

Provides the S. Lycopersicum metallothionein II-like protein (MTB) gene promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlMtb promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPTctp (GB0083)

Plasmid

#68169

Purpose

Provides the S. Lycopersicum translationally-controlled tumor protein homolog promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlTCTP promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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