We narrowed to 16,586 results for: EMB

1. pcDNA3.1-39S Heavy chain-SpyTag003

   Plasmid

   #216296

   Purpose

   Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   39S Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AA019

   Plasmid

   #216039

   Purpose

   Fragmid fragment: (C' terminus) VP64; NpNLS

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   VP64_v2.1; NpNLS_v1.1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pmiRFP670-C1-Nir1-LNS2

   Plasmid

   #220085

   Purpose

   high affinity PA biosensor with far-red fluorophore

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Use

   Tags

   iRFP670-GGSGGM

   Expression

   Mammalian

   Mutation

   amino acids 613-897

   Promoter

   CMV

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGM258

   Plasmid

   #220176

   Purpose

   Expresses exogenous C-terminal mutant TTC1 (I271D)

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TTC1 (TTC1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   I271D

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGM133

   Plasmid

   #220173

   Purpose

   CRISRPi guide RNA against TTC1

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TTC1 (sgRNA) (TTC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKL2-138

   Plasmid

   #219413

   Purpose

   Estradiol-inducible expression of 42-10 Zinc finger activator

   Depositor

   Ahmad Khalil

   Article

   Bragdon et al Cell. 2023 Aug 31;186(18):3810-3825.e18.

   Insert

   {pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Four Arg to Ala mutations in zinc finger backbone

   Promoter

   Available since

   May 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGM268

   Plasmid

   #220181

   Purpose

   Expresses exogenous C-terminal mutant TTC1 (I271D) with an N-terminal sfGFP tag

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TTC1 (TTC1 Human)
   

   Use

   Lentiviral

   Tags

   sfGFP

   Expression

   Mutation

   I271D

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGM265

   Plasmid

   #220179

   Purpose

   Expresses exogenous wild-type TTC1 with an N-terminal sfGFP tag

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TTC1 (TTC1 Human)
   

   Use

   Lentiviral

   Tags

   sfGFP

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGM216

   Plasmid

   #220175

   Purpose

   Expresses exogenous TPR mutant TTC1 (R190A_R194A)

   Depositor

   Jonathan Weissman

   Article

   Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

   Insert

   TTC1 (TTC1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   R190A + R194A

   Promoter

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_575

    Plasmid

    #216076

    Purpose

    Piggybac, constitutive expression destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Piggybac, destination

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNeonGreen-N1-Nir1-613-630

    Plasmid

    #220088

    Purpose

    N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Use

    Tags

    GGSGG-NeonGreen

    Expression

    Mammalian

    Mutation

    amino acids 613-630

    Promoter

    CMV

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AA018

    Plasmid

    #215976

    Purpose

    Fragmid fragment: (N' terminus) 4xVP16 transcritional activators

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1; VP64_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRDB_162

    Plasmid

    #216092

    Purpose

    Cas9 [Sp] CRISPRi targeting CD47, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD47 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKH160_FLAG-APEX2-YIPF4 LIR mut

    Plasmid

    #214979

    Purpose

    Flag-APEX2 tagged LIR mutant YIPF4

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    FLAG-APEX2_YIPF4_mut (YIPF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    LIR mutant

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKH145_mCherry-YIPF4 LIR mut

    Plasmid

    #214973

    Purpose

    mCherry tagged LIR mutant YIPF4

    Depositor

    Wade Harper

    Article

    Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

    Insert

    mCherry-YIPF4_mut (YIPF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    LIR mutant

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AA177

    Plasmid

    #215983

    Purpose

    Fragmid fragment: (N' terminus) Start codon only

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AA134

    Plasmid

    #216044

    Purpose

    Fragmid fragment: (C' terminus) SV40NLS; Linker; MeCP2

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    SV40NLS_v1.7; Linker_v4.1; MeCP2_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AA006

    Plasmid

    #216056

    Purpose

    Fragmid fragment: (2A Selection) Blasticidin resistance

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    T2A_v1.1; BlastR_v1.1; Stop
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AA199

    Plasmid

    #216050

    Purpose

    Fragmid fragment: (C' terminus) p65; NpNLS

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    p65_v1.1; NpNLS_v1.1
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AA266

    Plasmid

    #216064

    Purpose

    Fragmid fragment: (2A Selection) TagRFP

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    P2A_v1.1; TagRFP_v1.1; Stop
    

    Use

    Fragment

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge