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pPI192

Plasmid

#113219

Purpose

generic knockin vector for C-terminal tagging with mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mCherry

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pWS936

Plasmid

#123050

Purpose

G_ subunit for creating C-terminal transplants

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Gpa1 C-terminal Truncation Vector [3-4a]

Expression

Yeast

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 L892W

Plasmid

#1308

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L892W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 892 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 F130W

Plasmid

#1302

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 F130W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Phe 130 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 L553W

Plasmid

#1306

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 L553W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Leu 553 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pYO759

Plasmid

#235758

Purpose

Expression of FL ScVPS38-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

3xFLAG

Expression

Yeast

Promoter

ScVPS38 promoter

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pYO831

Plasmid

#235754

Purpose

Protein expression of ZZ-ScVPS38 (1-212F)

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 1-212F

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO225

Plasmid

#235729

Purpose

Protin expression of FL yeast VPS15 -ZZ in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS15

Tags

3xTEV-ZZ

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO290

Plasmid

#235731

Purpose

Protein expression in FL yeast VPS34 D731N mutant in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Expression

Yeast

Mutation

S2A, D731N

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO478

Plasmid

#235757

Purpose

Expression of FL ScVPS34-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Tags

3xFLAG

Expression

Yeast

Promoter

ScVPS34 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pYO764

Plasmid

#235761

Purpose

Expression of ScVPS38-EGFP under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

EGFP

Expression

Yeast

Promoter

ScVPS38 promoter

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pIT6EG7mlh

Plasmid

#185887

Purpose

In vivo gene amplification (HapAmp) of limonene synthetic module at RPL25 locus (23 copy).

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

see comments

Expression

Yeast

Mutation

ERG20 F96W N127W

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILAC2

Plasmid

#185889

Purpose

In vivo gene amplification (HapAmp) of lycopene synthetic module at RPL25 locus

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

see comments

Expression

Yeast

Mutation

ERG20 F96C; Xd.CRtYB E83K

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIT6EG7m

Plasmid

#185885

Purpose

A backbone plasmid for in vivo gene amplification (HapAmp) of a monoterpene synthetic module (+ GPP synthase without a monoterpene synthase) at RPL25 locus (14 copy)

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDM1355

Plasmid

#108994

Purpose

generic knockin vector for C-terminal tagging with GFP

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

GFP

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y257W

Plasmid

#1303

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y257W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 257 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pJC45BS104 Y507W

Plasmid

#1305

Depositor

Susan Lindquist

Article

Cashikar et al Mol Cell 2002 Apr;9(4):751-60.

Insert

Hsp104 Y507W (HSP104 Budding Yeast)

Tags

10X His tag

Expression

Bacterial

Mutation

Changed Tyr 507 to Trp

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pCMV-Cre

Plasmid

#123133

Purpose

Mammalian expression vector for wild-type Cre.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

Cre recombinase

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-EGFP

Plasmid

#52343

Purpose

AAVS1 donor vector for inducible gene expression

Depositor

Su-Chun Zhang

Article

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

EGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)IRES GFP

Plasmid

#51406

Purpose

Expresses GFP from internal ribosome entry sequence, cloning component

Depositor

Kathleen_L Collins

Article

Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

Insert

IRES GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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