We narrowed to 13,211 results for: CAR

1. pOTTC589 - pAAV c-fos Nuc-eYFP

   Plasmid

   #59133

   Purpose

   An AAV vector that expresses nuclear localized eYFP under control of the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   Nuclear-localized Enhanced Yellow Fluorescent Protein
   

   Use

   AAV

   Tags

   3xNLS

   Expression

   Mammalian

   Promoter

   mFos

   Available Since

   Oct. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3 1 kb prom. + Enhancer CD274

   Plasmid

   #107005

   Purpose

   Modified Luciferase expression vector

   Depositor

   Julian Downward

   Article

   Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

   Insert

   CD274 (CD274 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   N.B. There are two published SNPs in this region …

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMega-MaFRSA

   Plasmid

   #200226

   Purpose

   The plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with meta-trifluoromethylphenylalanine..

   Depositor

   Alanna Schepartz

   Article

   Fricke et al Nat Chem. 2023 Jun 1. doi: 10.1038/s4155

   Inserts

   Pyrrolysyl-tRNA synthetase
   pyrrolysyl-tRNA
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   mutated Asparagine 166 to Alanine and Valine 168 …

   Promoter

   proK-lacO and tacI

   Available Since

   June 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. VN-SOD1

   Plasmid

   #89757

   Purpose

   expresses human SOD1 fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Brasil et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   SOD1 (SOD1 Human)
   

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSET-iGluSnFR4s

   Plasmid

   #234443

   Purpose

   Bacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s
   

   Tags

   6xHis-Xpress epi

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRM049 His-TEV-Cas9 triNLS 2C

   Plasmid

   #196245

   Purpose

   Expression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)

   Depositor

   Ross Wilson

   Article

   Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   HA, His6 (N terminal on backbone), NLS, Nucleopla…

   Expression

   Bacterial

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATP1A1_plasmid_donor_RD

   Plasmid

   #86551

   Purpose

   Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

   Depositor

   Yannick Doyon

   Article

   Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

   Insert

   ATP1A1 (ATP1A1 Human)
   

   Use

   CRISPR and TALEN; Co-selection via hdr using ouab…

   Expression

   Mammalian

   Mutation

   Q118R, N129D

   Available Since

   March 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pInducer20 2xHA GPR153

   Plasmid

   #226812

   Purpose

   Tet-inducible lentiviral vector for 2xHA GPR153 expression

   Depositor

   Ramiro Iglesias-Bartolome

   Article

   Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

   Insert

   GPR153 (GPR153 Human)
   

   Use

   Lentiviral

   Tags

   2xHA tag followed by a GDPPVAT linker

   Mutation

   Sequence optimized from NP_997253.2

   Available Since

   May 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28b-ptetO::bac::gfp

   Plasmid

   #217882

   Purpose

   expresses the bac-BGC in bacterial cells, produces bacillamide D

   Depositor

   Tobias A. M. Gulder

   Article

   Hohmann et al Commun Biol. 2024 May 28;7(1):655. doi:

   Inserts

   Dehydrogenase
   Non-ribosomal peptide synthetase
   Aminotransferase
   

   Expression

   Bacterial

   Promoter

   ptetO

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAGT9569

    Plasmid

    #219431

    Purpose

    Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)
    

    Use

    CRISPR and Synthetic Biology; Moclo compatible le…

    Tags

    PapE-4LF2

    Expression

    Plant

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST

    Plasmid

    #208040

    Purpose

    Enables constitutive expression of N-terminal TurboID-fused PML (isoform IVa; WT); selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, TurboID

    Promoter

    EFS

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. MYC-PGK-blast

    Plasmid

    #190618

    Purpose

    Lentiviral expression plasmid of human MYC, blast selection

    Depositor

    Junwei Shi

    Article

    Cao et al Mol Cell. 2021 Sep 2;81(17):3604-3622.e1

    Insert

    MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. eSpCas9(1.1)_No_FLAG_ATP1A1_G2_Dual_sgRNA

    Plasmid

    #86612

    Purpose

    Vector for tandem expression of ATP1A1 G2 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos for second sgRNA using BbsI sites. Px333-like plasmid

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA + FLAGless enhanced specificity Cas9 (1.1)
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Expression

    Mammalian

    Mutation

    K848A, K1003A, & R1060A

    Promoter

    CBh

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

    Plasmid

    #107009

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas9_sgRNA_0

    Plasmid

    #70763

    Purpose

    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating

    Depositor

    Regine Kahmann

    Article

    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10

    Inserts

    U6 promoter
    cas9
    tnos terminator
    

    Use

    Self-replicating in ustilago maydis, conferring c…

    Tags

    N-terminal NLS, C-terminal HA-tag +NLS

    Mutation

    Streptococcus pyogenes gene codon-optimized for U…

    Promoter

    U6 from Ustilago maydis and synthetic otef promot…

    Available Since

    Nov. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOTTC337 - pAAV EF1a DIO mCherry

    Plasmid

    #47636

    Purpose

    An AAV vector that expresses mCherry in a Cre-dependent manner (DIO, double floxed inverted open reading frame).

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    mCherry
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. acta2_mCherry_pA_pDestTolpA2

    Plasmid

    #78685

    Purpose

    acta2:mCherry transgenesis construct

    Depositor

    Sarah Childs

    Article

    Whitesell et al PLoS One. 2014 Mar 3;9(3):e90590. doi: 1

    Insert

    acta2 promoter (acta2 Zebrafish)
    

    Use

    Unspecified

    Tags

    mCherry

    Promoter

    acta2

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3-EF1aH-TAZ S89A

    Plasmid

    #179910

    Purpose

    Construct for expressing an activated form of TAZ (encoding be gene WWTR1)

    Depositor

    Xin Chen

    Article

    Wang et al J Hepatol. 2021 Aug 28. pii: S0168-8278(

    Insert

    WW Domain Containing Transcription Regulator 1 (WWTR1 Human)
    

    Tags

    3X Flag-tag

    Expression

    Mammalian

    Mutation

    Serine 89 mutated to Alanine

    Promoter

    EF1a

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRIPZ Myc2-mouse TTP aa S52 S178 MUT

    Plasmid

    #107001

    Purpose

    Expresses epitope-tagged double-mutant murine TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (Zfp36 Mouse)
    

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Mutation

    Changed Ser52 to Ala and Ser178 to Ala

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCW57.1-FLAG-SRSF3_Full.Length_Codon.Optimized-Blast

    Plasmid

    #171951

    Purpose

    All-in-one lentiviral vector for doxycycline-inducible expression of FLAG-tagged, codon-optimized, full-length SRSF3

    Depositor

    Sujatha Jagannathan

    Article

    Campbell et al Cell Rep. 2023 Jun 13;42(6):112642. doi:

    Insert

    serine and arginine rich splicing factor 3 (SRSF3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    TRE

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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