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Showing: 9021 - 9040 of 25608 results
  1. AAV-TRE-NS1-dTomato

    Plasmid
    #175278
    Purpose
    AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato
    Depositor
    Insert
    NS1; dTomato (POLY Yellow fever virus strain 17D)
    Use
    AAV
    Tags
    dTomato (from bidirectional promoter)
    Promoter
    bidirectional TRE promoter
    Available Since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. FRIG-myc-Sema4D-CD4

    Plasmid
    #51606
    Purpose
    expresses myc-tagged Sema4D/CD4 non-cleavable chimera with extracellular Sema4D, intracellular CD4 in lentiviral backbone
    Depositor
    Insert
    Sema4D/CD4-FLAG (Sema4d Human, Mouse)
    Use
    Lentiviral
    Tags
    FLAG and myc
    Expression
    Mammalian
    Mutation
    Sema4D amino acids 660-861 were replaced with CD4…
    Promoter
    RSV
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pelB-MBP-BB0238-ss

    Plasmid
    #137046
    Purpose
    E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage
    Depositor
    Insert
    AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
    Tags
    PelB signal peptide + His10 + maltose binding pro…
    Expression
    Bacterial
    Mutation
    mature BB0238: lacks the BB0238 signal peptide
    Promoter
    T7lac
    Available Since
    April 22, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pLKO_HA.V5.FLAG_NGFR

    Plasmid
    #158250
    Purpose
    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).
    Depositor
    Insert
    Pro-Code Tagged human dNGFR (NGFR Human)
    Use
    CRISPR and Lentiviral
    Tags
    HA.V5.FLAG
    Mutation
    Truncation of the signaling domain
    Promoter
    EF1a
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 9021 - 9040 of 25608 results