We narrowed to 18,937 results for: Tec

1. TRIM28 gRNA (BRDN0001145369)

   Plasmid

   #76141

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TRIM28

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TRIM28 (TRIM28 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Venus-cpVenus-FLARE-AKAR

   Plasmid

   #123329

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-AKAR
   

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pICE-HA-Ku70-siR-WT

   Plasmid

   #82329

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

   Insert

   Ku70 (XRCC6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV-tet

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Circular 200,100 (mPCSK9)

   Plasmid

   #170122

   Purpose

   AAV vector carrying 2 copies of a guide RNA targeting the mouse PCSK9 mRNA, expressed from human and mouse U6 promoters

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

   Insert

   Circular 200,100 guide RNA (Pcsk9 Mouse)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Human U6 and mouse U6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. TBK1 gRNA (BRDN0001148607)

   Plasmid

   #76363

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human TBK1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   TBK1 (TBK1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES-hrGFP II-mTET1_CD

   Plasmid

   #83571

   Purpose

   Expresses a mutant catalytic domain of TET1 in mammalian cells

   Depositor

   Jean-Pierre Issa

   Article

   Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

   Insert

   Tet Methylcytosine Dioxygenase 1 (TET1 Human)
   

   Tags

   3X FLAG tag and hr GFP II

   Expression

   Mammalian

   Mutation

   TET1 amino acids 1-1417 deleted, catalytic domain…

   Promoter

   CMV

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYTSK01K_0G7

   Plasmid

   #177296

   Purpose

   genomic integration of genes (inserted in I-SceI-site) via transposon Tn7 with GmR

   Depositor

   Karl-Erich Jaeger

   Article

   Tiso et al Front Bioeng Biotechnol. 2020 Aug 20;8:9

   Insert

   PaacC1-aacC1
   

   Use

   Synthetic Biology; Yeast expression, tn7 genomic …

   Expression

   Bacterial

   Available Since

   Aug. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PRKCE gRNA (BRDN0001148049)

   Plasmid

   #76791

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRKCE

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRKCE (PRKCE Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_UdgX-APOBEC1-UdgX-HF-NG-nCas9

   Plasmid

   #163561

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-APOBEC1-UdgX-HF-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Venus-cpVenus-FLARE-AKAR(T/A)

    Plasmid

    #123330

    Purpose

    Negative control mutant for Venus-cpVenus-FLARE-AKAR biosensor.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR(T/A)
    

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Target Thr residue in substrate domain mutated to…

    Promoter

    CMV

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX307-SARS-CoV-2-3CL-C145A

    Plasmid

    #160279

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C145A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-BE4GamRA-P2A-Puro

    Plasmid

    #112673

    Purpose

    Lentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE4GamRA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    NLS sequence at the N-terminus and D10A

    Promoter

    EF1s

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-Foxo1-ADA 6KQ

    Plasmid

    #17563

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 ADA 6KQ (Foxo1 Mouse)
    

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    K242, K245, K259, K262, K271 and K291 were replac…

    Promoter

    CMV

    Available Since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pBK086

    Plasmid

    #183145

    Purpose

    pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

    Depositor

    Daan Swarts

    Article

    Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

    Insert

    MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)
    

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-2X-P2A-Puro

    Plasmid

    #110840

    Purpose

    Lentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3RA-2X
    

    Use

    Lentiviral

    Mutation

    D10A and 2 tandem NLS sequences in the XTEN linker

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. miniCGBE1-VRQR (pBM1173)

    Plasmid

    #140255

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)_VRQR-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQE80L-SpyCatcher-ELP-GFP

    Plasmid

    #69835

    Purpose

    Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

    Depositor

    Viviana Gradinaru

    Article

    Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

    Insert

    SpyCatcher-ELP-GFP
    

    Tags

    6x His Tag, GFP, and TEV Tag

    Expression

    Bacterial

    Promoter

    T5 promoter/lac operator element

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSpG-PPE

    Plasmid

    #170130

    Purpose

    For plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103

    Insert

    nCas9-SpG(H840A)-M-MLV
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    H840A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T1…

    Promoter

    maize Ubiquitin-1

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid

    #131313

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-HA-Flag-natT1R2 ECD-MHC

    Plasmid

    #113957

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R2 (TAS1R2 Human)
    

    Tags

    FLAG, Signal/leader sequence from influenza hemag…

    Expression

    Mammalian

    Mutation

    residues 22-568 fused to a transmembrane tether

    Promoter

    cmv

    Available Since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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