We narrowed to 8,823 results for: BLI;

1. AAV-CAG-M-SPOTIT2(R279F)

   Plasmid

   #191493

   Purpose

   Green fluorescent-based opioid sensor for the mu opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoter

   Depositor

   Wenjing Wang

   Insert

   M-SPOTIT2(R279F)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   R279F in OR

   Promoter

   CAG

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PAS_HeSAHS4

   Plasmid

   #215648

   Purpose

   Expresses SUMO-tagged HeSAHS4 protein from tardigrade H. exemplaris in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS4
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PAS_RvSAHS4

   Plasmid

   #215646

   Purpose

   Expresses SUMO-tagged RvSAHS4 protein from tardigrade R. varieornatus in E. coli

   Depositor

   Pamela Silver

   Insert

   SAHS4
   

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX458-tRNA-SpCas9

   Plasmid

   #195183

   Purpose

   Vector for transient Cas9 and EGFP expression. Small tRNA promoter for sgRNA cloning by GoldenGate.

   Depositor

   Stephanie Roessler

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   tRNA-Gln

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7CFE1_EVD68_VP12A_C107R

   Plasmid

   #212982

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A C107R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed cysteine 107 from 2A to Arginine

   Promoter

   T7

   Available since

   Feb. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7CFE1_EVD68_3ABC_C147R

   Plasmid

   #212978

   Purpose

   Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABC C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed Cysteine 147 in 3C to Arginine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7CFE1_EVD68_3ABCD_C147R

   Plasmid

   #212980

   Purpose

   Encodes EV-D68 MO/14/18947 3ABCD with active site mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 3ABCD C147R
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed cysteine 147 from 3C to arginine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7CFE1_EVD68_VP12A_N84T

   Plasmid

   #212983

   Purpose

   Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts

   Depositor

   Karla Kirkegaard

   Insert

   EV-D68 MO/14/18947 VP12A N84T
   

   Use

   Mammalian cell free protein expression

   Tags

   HA

   Expression

   Mutation

   changed Asparagine 84 from 2A to Threonine

   Promoter

   T7

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28-Flag-RBM24-1~93

   Plasmid

   #208633

   Purpose

   for E. coli expression of truncated Flag tagged human RBM24 (ENST00000379052), aa1~93

   Depositor

   Quanfu Ma

   Insert

   human RBM24 (ENST00000216146) aa 1~93 (RBM24 Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   only aa 1~93, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28-RPL3-Twstrep

    Plasmid

    #208623

    Purpose

    for E. coli expression of C-ter Twin Strep tagged human RPL3 (ENST00000216146)

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) (RPL3 Human)
    

    Use

    Tags

    6xHis and Twin Strep

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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