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pCAD131_hIrg1_M154I_pCMV6

Plasmid

#239120

Purpose

hACOD1 with M154I mutation in mammalian expression vector

Depositor

Konrad Buessow

Article

Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

Insert

cis-aconitate decarboxylase (ACOD1 Human)

Tags

Myc and FLAG

Expression

Mammalian

Mutation

M154I

Promoter

CMV

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-delta848-918

Plasmid

#238313

Purpose

ScVip1-536-1107-delta848-918 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A-H548A-R551A

Plasmid

#238317

Purpose

ScVip1-536-1107-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A-H548A-R551A

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGB1-tail-mTurquoise2

Plasmid

#145142

Purpose

Shuttle vector with mTurquoise2-tagged ITGB1 cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGB1-tail-mTurquoise2 (ITGB1 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet

Plasmid

#145143

Purpose

Shuttle vector with YPet-tagged ITGA2 wild-type cytoplasmic tail as an insert

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (ITGA2 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b ITGA2-tail-YPet (GFFKR/GAAKR)

Plasmid

#145144

Purpose

Shuttle vector with YPet-tagged ITGA2 cytoplasmic tail as an insert with a GFFKR/GAAKR mutation

Depositor

Johanna Ivaska

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

ITGA2-tail-YPet (GFFKR/GAAKR) (ITGA2 Human)

Use

Entry vector for gateway cloning

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-SOX17WT

Plasmid

#216176

Purpose

Generate lentiviruses encoding for wild-type SOX17

Depositor

Ralf Jauch

Article

Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

Insert

SOX17 (SOX17 Human)

Use

Lentiviral

Mutation

Glu113Glu (silent mutation); Glu120Glu (silent mu…

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-DeltaER

Plasmid

#238416

Purpose

Expresses GRP78 lacking the ER signal peptide in mammalian cells

Depositor

Amy Shiu Lee

Article

Liu et al J Biol Chem. 2025 Feb 28;301(4):108369.

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

The first 18 amino acids which is the ER signal p…

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A-K556A-K644A

Plasmid

#238328

Purpose

ScVip1-536-1107-K554A-K556A-K644A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A-K556A-K644A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-P553V-G646V-G647A

Plasmid

#238322

Purpose

ScVip1-536-1107-P553V-G646V-G647A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-P553V-G646V-G647A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K817S-D819A-S821A

Plasmid

#238326

Purpose

ScVip1-536-1107-K817S-D819A-S821A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K817S-D819A-S821A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-LEO1

Plasmid

#231727

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

LEO1 (LEO1 Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-PAF1

Plasmid

#231728

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

PAF1 (PAF1 Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-Cyclin T1

Plasmid

#231720

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CCNT1 (CCNT1 Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEIF3D-WT-sgNT

Plasmid

#222120

Purpose

EIF3D overexpression vector with non-targeting sgRNA

Depositor

Jonathan Weissman

Article

She et al Nucleic Acids Res. 2023 Jul 7;51(12):635

Insert

eIF3D (EIF3D Human)

Use

Lentiviral

Tags

BFP-P2A

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCM100_ PB5'IR-hU6-gRNA-CS1- CAG-tdTomato- PB3'IR

Plasmid

#229995

Purpose

PiggyBac sgRNA cloning plasmid with tdTomato reporter with a capture sequence (cs1)

Depositor

Christian Mayer

Article

Dvoretskova et al Nat Neurosci. 2024 May;27(5):862-872. do

Type

Empty backbone

Use

CRISPR

Tags

NotI site for NEBuilder HiFi DNA Assembly with ss…

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pJExpress401-KDAC8 D101N

Plasmid

#224348

Purpose

Expresses human KDAC8 (HDAC8) D101N in E. coli

Depositor

Terry Watt

Article

Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Aspartate 101 mutated to asparagine

Promoter

T5

Available Since

Feb. 19, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

Plasmid

#232246

Purpose

Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Mutation

delta73-82

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro -GFP-I77P I78P REX1-IRES-mCherry

Plasmid

#232245

Purpose

Mammalian expression of I77P I78P REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

I77P I78P REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Mutation

I77P I78P

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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11_T7-SP-mScarlet3-GPI

Plasmid

#229806

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and a GPI attachment site

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

SP-mScarlet3-GPI

Use

In vitro transcription

Mutation

T7 promoter

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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