We narrowed to 11,590 results for: 110

1. pMOD_A0701

   Plasmid

   #91019

   Purpose

   Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_H840A nickase, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-AtCas9_H840A nickase
   

   Use

   CRISPR

   Mutation

   H840A

   Promoter

   35S

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. SMT205

   Plasmid

   #134817

   Purpose

   SMT201 with mutated RBS for DHFR, in vivo assays

   Depositor

   Tanja Kortemme

   Article

   Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

   Inserts

   Dihydrofolate reductase
   Thymidylate synthase
   

   Expression

   Bacterial

   Mutation

   Silent mutations to eliminate internal restrictio…

   Promoter

   T7 and TET

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAPi

   Plasmid

   #127548

   Purpose

   Empty control for pGAPi-based RNAi silencing in tandem with APT transcript segment

   Depositor

   Luis Vidali

   Article

   Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

   Insert

   Adenine Phosphorybosyltransferase transcript segment
   

   Use

   RNAi; Empty control

   Promoter

   Ubiquitin

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. MyoUTipGAPi

   Plasmid

   #127549

   Purpose

   Positive RNAi control for pGAPi-based RNAi silencing in tandem with APT transcript segment

   Depositor

   Luis Vidali

   Article

   Orr et al Plant Physiol. 2020 Aug 6. pii: pp.20.00

   Insert

   Myosin XIa+b 5'UTR and Adenine Phosphorybosyltransferase transcript segment
   

   Use

   RNAi; Positive control

   Promoter

   Ubiquitin

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTC393

   Plasmid

   #91221

   Purpose

   protoplast vector expressing gRNA24 targeting tomato ANT1 (with TREX2 expression)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   gRNA targeting tomato ANT1
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTC363

   Plasmid

   #91211

   Purpose

   protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtU6 promoters)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   2 gRNAs targeting tomato ARF8A
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-HA-mIFITM3-C105A

   Plasmid

   #58392

   Purpose

   Expressed murine IFITM3-C105A with an N-terminal HA tag in mammalian cells

   Depositor

   Howard Hang, Jacob Yount

   Article

   Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Cysteine 105 to Alanine

   Promoter

   CMV IE

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

8. 824 pGEX2T PTEN delta274-342

   Plasmid

   #10739

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Deletion of amino acids 274-342.

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pCMV-myc-mIFITM3

   Plasmid

   #58458

   Purpose

   Expresses murine IFITM3 with an N-terminal myc tag in mammalian cells.

   Depositor

   Jacob Yount

   Article

   Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Sept. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. Cenp-B DBD INCENP TSS/AAA mCherry

    Plasmid

    #45234

    Purpose

    Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
    

    Tags

    CENP-B DBD, DNA binding domain of CENP-B and mChe…

    Expression

    Mammalian

    Mutation

    INCENP C-terminal TSS mutated to AAA

    Promoter

    t7

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. px330-MRPsg4

    Plasmid

    #87192

    Purpose

    disruption of human RMRP gene via CRISPR-Cas9, guide 4

    Depositor

    Thomas Cech

    Article

    Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

    Insert

    human MRP RNA CRISPR guide #4 (RMRP Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. phNFL-N98S

    Plasmid

    #132592

    Purpose

    Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

    Depositor

    Anthony Brown

    Article

    Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

    Insert

    NEFL (NEFL Human)
    

    Expression

    Mammalian

    Mutation

    293A>G; Asn98Ser

    Promoter

    CMV

    Available Since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pInducer21 Flag-Sesn1b

    Plasmid

    #61865

    Purpose

    Dox inducible lentivirial expression of Flag-Sesn1b (mouse)

    Depositor

    Ming Li

    Article

    Peng et al Cell. 2014 Sep 25;159(1):122-33. doi: 10

    Insert

    Sesn1 (Sesn1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pKP4010

    Plasmid

    #113336

    Purpose

    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Inserts

    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    

    Tags

    3X-FLAG/8X-Gly linker

    Expression

    Yeast

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

    Plasmid

    #46838

    Purpose

    Express cGFP transcript ending in the minimal functional MALAT1 triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. D-raf R217L

    Plasmid

    #39762

    Depositor

    Norbert Perrimon

    Article

    Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

    Insert

    d-raf (Raf Fly)
    

    Expression

    Insect

    Mutation

    R217L

    Available Since

    Jan. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pTC303

    Plasmid

    #91210

    Purpose

    protoplast vector expressing 2 gRNAs targeting tomato ARF8A (AtU6 and AtSL7 promoters)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    2 gRNAs targeting tomato ARF8A
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAtCas9-dead_3

    Plasmid

    #91167

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A+H840A (dead)
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    D10A, H840A

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJG484

    Plasmid

    #91194

    Purpose

    WDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi8+donor)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    nTaCas9_H840A+gUbi8+donor
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCUX1.1.0-gDNA

    Plasmid

    #112434

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor CUX1

    Depositor

    Kevin White

    Insert

    CUX1 (CUX1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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