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pcDNA5/FRT/TO GFP HSPA14

Plasmid

#19488

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPA14 (HSPA14 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBASH3B-1/1

Plasmid

#91462

Purpose

Protein expression and purification of human SH3 domain construct UBASH3B-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

UBASH3B-1/1 (UBASH3B Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMX-Ascl1-puro

Plasmid

#198227

Purpose

Retroviral expression of Ascl1

Depositor

Li Qian

Article

Wang et al Cell Stem Cell. 2022 Oct 6;29(10):1491-1

Insert

Ascl1 (Ascl1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-BBS4

Plasmid

#218713

Purpose

Expresses N-terminally EGFP-tagged BBS4 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS4 (BBS4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

I354T (t1110c)

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

Plasmid

#197979

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-vCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 SPASTIN , residues 112-196

Plasmid

#180627

Purpose

Bacterial expression for SPASTIN MIT, residues 112-196. Has N-term HIS-SUMO tag. Used for X-ray crystallography. Internal ID:WISP21-111.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 112-196

Promoter

T7

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Epsin 2

Plasmid

#22241

Depositor

Pietro De Camilli

Article

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
pTU425

Plasmid

#178984

Purpose

pACYC containing Candidatus “Scalindua brodae” TPR-CHAT H585A C627A (codon-optimized)

Depositor

Stan Brouns

Article

van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

Insert

TPR-CHAT H585A C627A

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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ELF5_pet28a

Plasmid

#131659

Purpose

Bacterial expression of ETS gene ELF5

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ELF5 (ELF5 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-puro GFP-UVRAG

Plasmid

#38267

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

UV radiation resistance-associated gene protein (UVRAG Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only
pSVL-mo FceRI gamma

Plasmid

#8372

Depositor

Jean Kinet

Article

Ra et al J Biol Chem 1989 Sep 15;264(26):15323-7.

Insert

mouse Fc epsilon RI gamma cDNA (Fcer1g Mouse)

Expression

Mammalian

Available Since

June 7, 2005

Availability

Academic Institutions and Nonprofits only
ZIKV_Trigger_32B

Plasmid

#75009

Purpose

Portion of Zika Virus genome (KU312312: 7166-7299) containing sensor 32B trigger sequence

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_32B

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSR284

Plasmid

#125093

Purpose

Expression of ompR(1-122aa)-manR(120-234aa) chimera under PLtetO-1, output Promoter PmntCAB143

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-manR(DBD)122
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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psgRNA (GB0645)

Plasmid

#68210

Purpose

Provides a Short guide RNA which is the combination of the bacterial crRNA and tracrRNA into a single guide transcript; it does not contain any target site

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SgRNA

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Sfi1

Plasmid

#136793

Purpose

Mammalian expression of the centrosomal protein hSfi1 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-hSfi1 (SFI1 Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-OC43-nsp1

Plasmid

#168931

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp1 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp1

Plasmid

#168893

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp1 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-nsp1

Plasmid

#168841

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp1 (ORF1ab )

Use

Gateway-compatible entry vector

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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NG0750 pCI-TPI-PTC48-4H

Plasmid

#65803

Purpose

NMD reporter mRNA

Depositor

Niels Gehring

Article

Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

Insert

TPI (TPI1 Human)

Tags

4H (4 copies of short beta-globin 3'UTR for …

Expression

Mammalian

Mutation

premature stop codon at 48

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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