We narrowed to 18,671 results for: Met;

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pICH47742:PtFCP:Cas9YFP

Plasmid

#104894

Purpose

Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

Cas9

Use

CRISPR; ; golden gate assembly

Tags

YFP

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSECB

Plasmid

#106305

Purpose

Express spCas9 with sgRNA, blast resistance

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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Dendra2-Zyxin-6

Plasmid

#57750

Purpose

Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Zyxin (ZYX Human)

Tags

Dendra2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pILGFPQ3

Plasmid

#83558

Purpose

Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL2 promoter-yEGFP

Expression

Yeast

Promoter

GAL2

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot2

Plasmid

#79255

Purpose

Expresses EGFP-tagged DmNot2 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot2 (Rga Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot3

Plasmid

#79250

Purpose

Expresses EGFP-tagged DmNot3 in S2 cells

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

DmNot3 (Not3 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1wt-PA-mCherry

Plasmid

#138252

Purpose

Wild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry).

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1-PAmCherry

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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SEPLuc

Plasmid

#141102

Purpose

bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEP-Nanoluc

Use

Lentiviral

Promoter

EF1α

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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A102-GAP-BoCCS-GRM

Plasmid

#123286

Purpose

Expression of Glo3 ΔAmpH (1-459) in E.Coli

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

ADP-ribosylation factor GTPase-activating protein (GLO3 Budding Yeast)

Tags

His and MBP

Expression

Bacterial

Promoter

T5

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA_(1,20,6)-GFP

Plasmid

#124619

Purpose

Expresses adRNA (1,20,6) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(1,20,6)

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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SI15-CD276

Plasmid

#46305

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

CD276 mouse monoclonal antibody (cd276 Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
CALCOCO1 V142A-mycDDK

Plasmid

#137768

Purpose

CALCOCO1 CLIR mutant

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

CALCOCO1 V142A

Tags

mycDDK

Expression

Mammalian

Mutation

V142A

Promoter

CMV

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSB4C5_J23101-CocE

Plasmid

#128129

Purpose

Expressing CocE enzyme (cocaine to benzoate) gene under control of the constitutive promoter J23101, and RBS B0032

Depositor

Jean-Loup Faulon

Article

Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

Insert

CocE

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23101

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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SI6-IGSF21a

Plasmid

#36141

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

IGSF21a mouse monoclonal antibody (igsf21a Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE intramol-1m

Plasmid

#13551

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIP-E intramol A207W (gltI E. coli)

Tags

EYFP

Expression

Bacterial

Mutation

A207W, ECFP insert in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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As5 (pBW414J105-arsR)

Plasmid

#123162

Purpose

Arsenic sensor used in the sensor cell array. pSB3K3 carrying J105-30arsR-t-ParsR-30gfp-t

Depositor

Baojun Wang

Article

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J105-30arsR-t-ParsR-30gfp-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J105, ParsR

Available Since

Sept. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Coho salmon GLUT4 (okGLUT4)

Plasmid

#65102

Purpose

Full length SLC2A4 cDNA (Oncorhynchus kisutch)

Depositor

Josep Planas

Article

Capilla et al Am J Physiol Endocrinol Metab. 2004 Aug;

Insert

SLC2A4

Expression

Bacterial

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 G260E mutant

Plasmid

#67610

Purpose

bacterial expression of GST-PRMT9 G260E Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

G260E double E loop mutant

Promoter

tac

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
GST-PRMT9 D258G mutant

Plasmid

#67609

Purpose

bacterial expression of GST-PRMT9 D258G Double E loop mutant

Depositor

Steven Clarke

Article

Hadjikyriacou et al J Biol Chem. 2015 May 15. pii: jbc.M115.

Insert

PRMT9 (PRMT9 Human)

Tags

GST

Expression

Bacterial

Mutation

D258G double E loop mutant

Promoter

tac

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA-TAL-NC2

Plasmid

#43855

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Tags

Flag and FokI nuclease

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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