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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,648 results for: Ada;

Showing: 9141 - 9160 of 9648 results

  1. pVH009

    Plasmid
    #80399
    Purpose
    Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    3xFLAG-Venus YFP-AAV
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    C-terminal LZx domain and N-terminal 3xFLAG tag, …
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    80399-plasmid-map-sequence-id-155900

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  4. TLCV2

    Plasmid
    #87360
    Purpose
    LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive sgRNA expression.
    Depositor
    Adam Karpf
    Article
    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:
    Has Service
    Cloning Grade DNA
    Insert
    Cas9-2A-eGFP
    Use
    CRISPR and Lentiviral; Doxycycline inducible; egf…
    Tags
    Cas9-T2A-eGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Tight TRE promoter
    Available since
    Feb. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87360-plasmid-map-sequence-id-473508

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  6. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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  9. pSAM_AraC

    Plasmid
    #91569
    Purpose
    Mariner transposon mutagenesis & sequencing vector - Transposon contains KanR and Illumina sequencing adapters. Arabinose promoter expresses transposase. Ampicillin resistance on backbone.
    Depositor
    Harry Mobley
    Article
    Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.
    Insert
    AraC/PBAD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91569-plasmid-map-sequence-id-181631

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  12. pCW57-GFP-P2A-MCS (Neo)

    Plasmid
    #89181
    Purpose
    All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    Lentiviral; Doxycycline inducible; p2a self cleav…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    April 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    89181-plasmid-map-sequence-id-176294

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  13. PA-Dre

    Plasmid
    #169706
    Purpose
    Photoactivatable Dre recombinase for AAV production and expression in mammalian cells
    Depositor
    Dali Li
    Article
    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11
    Insert
    Dre246-nMag-T2A-pMag-Dre247
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CAG
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    169706-plasmid-map-sequence-id-333284

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  16. AYBEv3

    Plasmid
    #193967
    Purpose
    An engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter
    Depositor
    Huawei Tong
    Article
    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038
    Insert
    ecTadA8e-nSpCas9(D10A)-MPG(v3)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G163R, N169S, S198A, K202A, G203A, S206A, K210A
    Promoter
    hU6, EF1a, CBh
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    193967-plasmid-map-sequence-id-381640

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  19. pLPT234

    Plasmid
    #127855
    Purpose
    Repressilator 2.0, without degradation tags, integrated triple reporter
    Depositor
    Johan Paulsson
    Article
    Riglar et al Nat Commun. 2019 Oct 11;10(1):4665. doi:
    Inserts
    bla
    mSCFP3
    mKate2
    mVenus
    TetR
    LacI
    cI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    First 11aa derived from mCherry as fusion protein
    Promoter
    pAmpR, pL LacO1, pL tetO1, and pR
    Available since
    July 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    127855-plasmid-map-sequence-id-253039

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Showing: 9141 - 9160 of 9648 results