Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 16,010 results for: LEA

Clear keywords
Showing: 9141 - 9160 of 16010 results

  1. CPH3736_GST-TEV-Spt6p-tSH2-1223-1454

    Plasmid
    #227088
    Purpose
    Bacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H, K1355A, K1435A
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227088-plasmid-map-sequence-id-451126

    Enlarge

  2. pET24a MDH_HALMA

    Plasmid
    #204241
    Purpose
    Bacterial expression of H. marismortui (Dead Sea halophilic red archaeon) cytosolic malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_HALMA (mdh Haloarcula marismortui)
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204241-plasmid-map-sequence-id-413344

    Enlarge

  3. lenti-Cas9-Exo-Blast-BFP

    Plasmid
    #196721
    Purpose
    Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.
    Depositor
    Bernhard Schmierer
    Insert
    Cas9-Exo1-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Promoter
    EF1a
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196721-plasmid-map-sequence-id-389753

    Enlarge

  4. pT2iC6FAST1nls2ER4(#638)

    Plasmid
    #184068
    Purpose
    cyclofen-inducible YFAST nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Insert
    YFAST-2nls-ERT2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available Since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    184068-plasmid-map-sequence-id-365820

    Enlarge

  5. pFLAG GLTPD2

    Plasmid
    #170745
    Purpose
    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.
    Depositor
    Rhoderick Brown
    Insert
    GLTPD2 (GLTPD2 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    V49F, V152A and D209E- Please see depositor comme…
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170745-plasmid-map-sequence-id-337083

    Enlarge

  6. pKP1411

    Plasmid
    #167638
    Purpose
    Express FepA I14C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA I14C
    Expression
    Bacterial
    Mutation
    fepA with I14C mutation (Mutation is I36C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167638-plasmid-map-sequence-id-339563

    Enlarge

  7. pKP1383

    Plasmid
    #167639
    Purpose
    Express FepA V15C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA V15C
    Expression
    Bacterial
    Mutation
    fepA with V15C mutation (Mutation is V37C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167639-plasmid-map-sequence-id-339571

    Enlarge

  8. pKP1384

    Plasmid
    #167641
    Purpose
    Express FepA V16C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA V16C
    Expression
    Bacterial
    Mutation
    fepA with V16C mutation (Mutation is V38C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167641-plasmid-map-sequence-id-339577

    Enlarge

  9. pKP1416

    Plasmid
    #167643
    Purpose
    Express FepA T17C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA T17C
    Expression
    Bacterial
    Mutation
    fepA with T17C mutation (Mutation is T39C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167643-plasmid-map-sequence-id-339580

    Enlarge

  10. pKP1583

    Plasmid
    #167645
    Purpose
    Express FepA D34C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA D34C
    Expression
    Bacterial
    Mutation
    fepA with D34C mutation (Mutation is D56C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167645-plasmid-map-sequence-id-339586

    Enlarge

  11. pKP1382

    Plasmid
    #167647
    Purpose
    Express FepA G54C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA G54C
    Expression
    Bacterial
    Mutation
    fepA with G54C mutation (Mutation is G76C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167647-plasmid-map-sequence-id-339587

    Enlarge

  12. pKP718

    Plasmid
    #167636
    Purpose
    Express FepA T13C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA T13C
    Expression
    Bacterial
    Mutation
    fepA with T13C mutation (Mutation is T35C based o…
    Available Since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167636-plasmid-map-sequence-id-339560

    Enlarge

  13. pKP1410

    Plasmid
    #167634
    Purpose
    Express FepA D12C . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Article
    Postle et al bioRxiv 2021.12.08.471779
    Insert
    fepA D12C
    Expression
    Bacterial
    Mutation
    FepA with D12C mutation. (Numbering based on star…
    Available Since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167634-plasmid-map-sequence-id-337287

    Enlarge

  16. psubCMV-WPRE-IL3SP-mVEGF-D-dNdC

    Plasmid
    #119229
    Purpose
    Plasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.
    Depositor
    Kari Alitalo
    Insert
    IL3SP-Vegfd-dNdC (Vegfd Mouse)
    Use
    AAV
    Promoter
    CMV
    Available Since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119229-plasmid-map-sequence-id-239316

    Enlarge

  19. STK10 (STK10A-c043)

    Plasmid
    #110255
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    STK10 (STK10 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110255-plasmid-map-sequence-id-212331

    Enlarge

  20. pVP16-tev-PRT1-C195A

    Plasmid
    #83408
    Purpose
    bacterial expression vector pVP16 containing a mutant variant of Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    C195A substitution
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    83408-plasmid-map-sequence-id-175084

    Enlarge

Showing: 9141 - 9160 of 16010 results