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pMXs-EGFP-ERas C220S/C222S-IP

Plasmid

#13829

Depositor

Shinya Yamanaka

Publication

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas C220S/C222S (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

C220 and C222 changed to serine residues

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1L16A/L17A

Plasmid

#13478

Depositor

Anton Bennett

Publication

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1L16A/L17A (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Leucine 16 to Alanine; changed Leucine 1…

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1 ASA

Plasmid

#13479

Depositor

Anton Bennett

Publication

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1ASA (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Changed Arginine 53 to Alanine, Changed Arginine…

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
HOXA10 (human) bluescript

Plasmid

#8617

Depositor

Corey Largman

Publication

Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

Insert

HOXA10 (HOXA10 Human)

Expression

Bacterial

Mutation

improved Ribosomal binding site (NcoI site) at AT…

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pRetroX-TRE3G_CBP-GFP

Plasmid

#221926

Purpose

Dox-inducible expression of CBP-GFP in mammalian cells by retroviral transduction

Depositor

Daniel Bose

Publication

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Use

Retroviral

Tags

GFP

Mutation

insertion of CBP-GFP

Available since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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SKIV2L

Plasmid

#156014

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SKIV2L (SKIC2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

D3E

Promoter

EF-1a

Available since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

Plasmid

#182415

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Publication

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-DARPinOFF7

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLentiCRISPRv2 sgSp1

Plasmid

#174907

Purpose

Second generation sgRNA against Sp1

Depositor

Jane Clifford

Publication

Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

Insert

Specificity Protein 1 (SP1 Human)

Expression

Mammalian

Mutation

Missense mutation to mutate PAM sequence

Promoter

EFS-NS

Available since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pONSY-CoNMM:mCherry

Plasmid

#111878

Purpose

This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia.

Depositor

Inaki Ruiz-Trillo

Publication

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

Capsaspora N-Myristoylation motif (CoNMM) from the Src2 gene fused to mCherry

Use

Capsaspora owczarzaki

Tags

mCherry

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-FLAG-MAGE-2-429

Plasmid

#89090

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Publication

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only
pCDNA-FRT-FAK-kinase30

Plasmid

#59124

Purpose

FAK fret control sensor without FERM domain with 30 nm linker

Depositor

Sivaraj Sivaramakrishnan

Publication

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX4T1-GST-eIF4G1-His6

Plasmid

#37231

Depositor

Jon Lorsch

Publication

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF4631 (TIF4631 Budding Yeast)

Tags

GST and His6

Expression

Bacterial

Promoter

tac

Available since

Sept. 4, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Publication

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-EQ-mycStrep

Plasmid

#31838

Depositor

Hemmo Meyer

Publication

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

E578Q

Promoter

CMV

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV-Tag2B Flag BimEL(TA)

Plasmid

#23093

Depositor

Roger Davis

Publication

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (TA) (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Threonine 112 to Alanine

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
Cdc42 T17N sp64T

Plasmid

#16672

Depositor

De-Li Shi

Publication

Djiane et al Development. 2000 Jul . 127(14):3091-100

Insert

Cdc42 T17N (CDC42 Human)

Use

Xenopus expression

Mutation

T17N. Dominant negative.

Available since

April 28, 2008

Availability

Academic Institutions and Nonprofits only
3441 pcDNA3 Bid G60A

Plasmid

#8775

Depositor

Stanley Korsmeyer

Publication

Zha et al Science 2000 Dec 1;290(5497):1761-5.

Insert

Bid p22 G60A (Bid Mouse)

Expression

Mammalian

Mutation

G60A

Available since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
Tet-O-FUW-NR4A1-P2A-mCherry

Plasmid

#203859

Purpose

Tet-inducible lentiviral plasmid expressing NR4A1-P2A-mCherry, with ORF specific barcode close to 3'LTR site

Depositor

Andrea Califano

Publication

Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

Insert

NR4A1 (NR4A1 Human)

Expression

Mammalian

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TERT

Plasmid

#156078

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TERT (TERT Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Type I inositol-1,4,5-trisphosphate 5-phosphatase in pcDNA3

Plasmid

#121976

Purpose

transfection studies in eukaryotic cells such as COS-7 cells

Depositor

Christophe Erneux

Publication

De Smedt et al J Biol Chem. 1996 Apr 26;271(17):10419-2

Insert

Type I Inositol-1,4,5-trisphoosphate 5-phosphatase (INPP5A Human)

Expression

Mammalian

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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