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pDONR223_SERPINB4_WT

Plasmid

#81766

Purpose

Gateway Donor vector containing SERPINB4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SERPINB4 (SERPINB4 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.F133S

Plasmid

#81637

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

F133S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast mAtg7

Plasmid

#173170

Purpose

Retroviral vector to express sgRNA resistant mouse Atg7

Depositor

Kivanc Birsoy

Article

Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

Insert

Autophagy Related 7 (Atg7 Mouse)

Use

Retroviral

Mutation

Synonymous mutations at sgRNA sites

Promoter

MMLV

Available Since

Feb. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_TBL1XR1_WT

Plasmid

#82059

Purpose

Gateway Donor vector containing TBL1XR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBL1XR1 (TBL1XR1 Human)

Use

Gateway entry vector

Mutation

L512P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-HA_N-human Prkd2 without kinase domain

Plasmid

#138411

Purpose

Express Prkd2 without kinase domain in mammalian cells

Depositor

Bruce Beutler

Article

Misawa et al Sci Immunol. 2020 Jan 24;5(43):eaaz0085.

Insert

Prkd2 without kinase domain (PRKD2 Human)

Tags

HA

Expression

Mammalian

Mutation

kinase domain deleted from human Prkd2

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-CD20-Puro

Plasmid

#209757

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGMC00005

Plasmid

#166722

Purpose

sgRNA against human ASNS

Depositor

Raj Chari

Article

Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

Insert

ASNS (ASNS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00006

Plasmid

#166723

Purpose

sgRNA against human ASNS

Depositor

Raj Chari

Article

Ruiz-Rodado et al Cancer Metab. 2020 Oct 21;8:23. doi: 10.

Insert

ASNS (ASNS Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.W131G

Plasmid

#81632

Purpose

Gateway Donor vector containing SPOP , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

W131G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCR4_miniFXN_7

Plasmid

#234360

Purpose

Mini FXN plasmid with a partial deletion of the 5' UTR and intron 1 in FXN. Does not contain GAA repeats.

Depositor

Marek Napierala

Article

Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

Insert

FXN (FXN Human)

Use

Cloning

Tags

Flag Tag

Mutation

There is a partial deletion in the 5'UTR of …

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_p.R171W

Plasmid

#81592

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

R171W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)

Plasmid

#145840

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_SPOP_p.F102C

Plasmid

#81642

Purpose

Gateway Donor vector containing SPOP, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SPOP (SPOP Human)

Use

Gateway entry vector

Mutation

F102C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PB-mCherry-DMDEx23-eGFP

Plasmid

#211366

Purpose

Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

Depositor

Ulrich Lächelt

Article

Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

Insert

mCherry-DMDEx23-eGFP

Expression

Mammalian

Mutation

mCherry interrupted by mdx dystrophin exon 23 bet…

Promoter

CAG

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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TDP-REGv2 Cryptic PE-Max

Plasmid

#216163

Purpose

Expression of prime editor PEMax (Plasmid #174820) only in cells with TDP-43 loss of function. Note: It is not recommended to produce lentivirus containing TDP-REG sequences – see Depositor Comments

Depositor

Pietro Fratta

Article

Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

Insert

PEMax for prime editing with internal cryptic exon

Use

CRISPR

Expression

Mammalian

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCR-XL-rAkap9

Plasmid

#196867

Purpose

Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Akap9 (Akap9 Rat)

Expression

Bacterial

Promoter

Lac promoter and T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAi14-luc-MC

Plasmid

#87113

Purpose

minicircle parental backbone plasmid including luc-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

Luciferase-SV40pA

Use

CRISPR and Luciferase

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBactin-EB3-mNeonGreen

Plasmid

#196848

Purpose

labelling of microtubule plus ends

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EB3-NeonGreen (MAPRE3 Human)

Expression

Mammalian

Mutation

None (wt)

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_WT

Plasmid

#81944

Purpose

Gateway Donor vector containing PPP2R1A, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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