We narrowed to 9,263 results for: CAG

1. CALM1 gRNA (BRDN0001148506)

   Plasmid

   #75764

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CALM1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CALM1 (CALM1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pU6-pegRNA-LRRK2-G2019S-3b

   Plasmid

   #180433

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pU6-pegRNA-LRRK2-G2019S-3c

   Plasmid

   #180434

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pU6-pegRNA-LRRK2-G2019S-3d

   Plasmid

   #180435

   Purpose

   Transiently expressing a pegRNA to introduce LRRK2-G2019S mutation in human cells

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   LRRK2-G2019S

   Promoter

   U6

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLKO-Tet-On-YAP1-shRNA1

   Plasmid

   #176846

   Purpose

   lentiviral vector for doxycycline-inducible human YAP1 knockdown

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   YAP1-Sh1 (YAP1 Human)
   

   Use

   Lentiviral

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AICSDP-45: TTN-mEGFP

   Plasmid

   #114412

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human TTN, via Cas9-excisable CAGGS-mCherry selection cassette

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

   Insert

   TTN Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TTN Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH

   Plasmid

   #131507

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hUBC

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

   Plasmid

   #184556

   Purpose

   Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

   Depositor

   Sandeep Prabhu

   Article

   Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

   Insert

   Kirsten rat sarcoma virus (Kras Mouse)
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Tags

   Flag

   Expression

   Bacterial

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV-KrasG12D/sgKras/Cre

   Plasmid

   #99851

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G12D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Anti-CASK [K56A/50R]

    Plasmid

    #177443

    Purpose

    Mammalian Expression Plasmid of anti-CASK (Human). Derived from hybridoma K56A/50.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-CASK (Homo sapiens) recombinant mouse monoclonal antibody (CASK Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pORANGE Gria3-GFP KI

    Plasmid

    #131491

    Purpose

    Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria3, Gria3 Mouse, Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pαH-S-GSAS-OMICRON.BA.2-RBD

    Plasmid

    #184830

    Purpose

    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6X His tag

    Expression

    Mammalian

    Mutation

    SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pαH-S-GSAS-OMICRON.BA.1-RBD

    Plasmid

    #184831

    Purpose

    Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.1 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

    Depositor

    Priyamvada Acharya

    Article

    Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

    Insert

    S-GSAS-OMICRON.BA.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    6X His tag

    Expression

    Mammalian

    Mutation

    SARS-CoV-2 Spike RBD protein of OMICRON.BA.1 vari…

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXPR_BRD051-sgMLH1-1

    Plasmid

    #125776

    Purpose

    constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgMLH1-1 (MLH1 Human)
    

    Use

    CRISPR

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXPR_BRD051-sgMLH1-2

    Plasmid

    #125777

    Purpose

    constitutive expression of a guide RNA targeting human MLH1 (CRISPR cutting control) and S. pyogenes Cas9

    Depositor

    Francisca Vazquez

    Article

    Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

    Insert

    sgMLH1-2 (MLH1 Human)
    

    Use

    CRISPR

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJT230 lenti pEF-rTetR(SE-G72P)-3XFLAG-NCOA3-TAD-T2A-mCherry-BSD-WPRE

    Plasmid

    #188763

    Purpose

    Lentiviral backbone containing NCOA3 as a fusion with rTetR(SE-G72P)-3XFLAG

    Depositor

    Michael Bassik, Lacra Bintu

    Article

    Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

    Insert

    NCOA3 (NCOA3 Human)
    

    Use

    Lentiviral

    Tags

    rTetR(SE-G72P)-3xFLAG

    Expression

    Mammalian

    Promoter

    pEF1a

    Available Since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-KrasG13C/sgKras/Cre

    Plasmid

    #99856

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13C mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-KrasG12R/sgKras/Cre

    Plasmid

    #99852

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G12R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXPR_BRD003 PSMB5 guide 1

    Plasmid

    #117073

    Purpose

    single guide RNA targeting PSMB5; guide 1

    Depositor

    William Hahn

    Article

    Hong et al Elife. 2019 Mar 12;8. pii: 44161. doi: 1

    Insert

    LMPX (PSMB5 Human)
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    March 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-KrasG13D/sgKras/Cre

    Plasmid

    #99857

    Purpose

    Expresses Cre-recombinase, a barcoded HDR template to introduce a G13D mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

    Depositor

    Monte Winslow

    Article

    Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

    Insert

    Kras (Kras Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge