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pEGFP-TRAF4(K190R)

Plasmid

#58319

Purpose

EGFP-tagged TRAF4 K190R mutant

Depositor

Ying Zhang

Article

Wang et al J Biol Chem. 2013 Jul 26;288(30):21784-9

Insert

TNF receptor associated factor 4 (Traf4 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

K 190 to R

Promoter

CMV

Available since

July 28, 2014

Availability

Academic Institutions and Nonprofits only
pLVX-mCherry-SPASTIN, L177D

Plasmid

#180649

Purpose

Lentiviral expression vector for SPASTIN. Has L177D mutation. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. SPASTIN starts on M87. Internal ID: WISP20-45.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

SPASTIN

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Starts at M87, L177D mutation, has siRNA resistan…

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-THTAP-VPS9D1, residues 1-97

Plasmid

#180640

Purpose

Bacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

VPS9D1 (C16orf7 Human)

Use

Tags

HIS-GST

Expression

Bacterial

Mutation

Residues 1-97

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNAL1 MIT residues 1-87

Plasmid

#180618

Purpose

Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNAL1 (KATNAL1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-87

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 KATNAL2 residues 1-98

Plasmid

#180619

Purpose

Bacterial expression for KATNAL2 , residues 1-98. Has N-terminal HIS-SUMO tag. Internal ID: WISP21-28.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNAL2 (KATNAL2 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 1-98

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCA528 RPS6KC1 MIT, residues 235-316

Plasmid

#180622

Purpose

Bacterial expression for RPS6KC1 MIT domain, residues 235-316. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-87.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

RPS6KC1 (RPS6KC1 Human)

Use

Tags

HIS-SUMO

Expression

Bacterial

Mutation

Residues 235-316

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Myc-KATNB1

Plasmid

#160054

Purpose

Mammalian expression vector for KATNB1, (Katanin p80) with N-terminal Myc tag. Internal ID: WISP20-16.

Depositor

Wesley Sundquist

Article

Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

Insert

KATNB1 (KATNB1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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gh109

Plasmid

#106768

Purpose

expression of gRNA targeting B3GALT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B3GALT6 (B3GALT6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Zeo_BACE1

Plasmid

#227105

Purpose

BACE1 enzyme overexpression for Nrg1 activation in luciferase and barcode assays

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

BACE1 (BACE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB8

Plasmid

#63109

Purpose

Expression of HSPB8 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb8 (HSPB8 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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