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pLenti-EF1alpha-IRES-EGFP-HA-hNPHP1

Plasmid

#122874

Purpose

Expresses HA-tagged human NPHP1 in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Insert

human NPHP1 (NPHP1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

EF1 alpha

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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Glycine receptor Alpha3 scFv [N424/45]

Plasmid

#206774

Purpose

Mammalian Expression of Glycine receptor Alpha3 scFV. Derived from hybridoma N424/45 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Glycine receptor Alpha3 (Homo sapiens) recombinant scFV (GLRA3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

Plasmid

#160624

Purpose

TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:MS2:VPR:Tnos

Use

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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Tet-Inducible Human Caspase-9 Alpha

Plasmid

#194708

Purpose

Inducible Expression of Human Caspase-9 Alpha

Depositor

Hannah Rabinowich

Article

Han et al J Biol Chem. 2014 Mar 7;289(10):6485-649

Insert

Human Caspase-9 Alpha (CASP9 Human)

Use

Tags

None

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS SARS-CoV-2 Alpha Spike

Plasmid

#185691

Purpose

Expresses codon-optimized full length SARS-CoV-2 Alpha Spike

Depositor

Marceline Côté

Article

Benlarbi et al iScience. 2022 Nov 18;25(11):105316. doi

Insert

SARS-CoV-2 Alpha Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Contains the following mutations: Δ69-70, Δ145, N…

Promoter

chicken β-actin promoter, CMV enhancer

Available since

June 8, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pBa.GFP-KIF1Balpha tail 387-1150

Plasmid

#134619

Purpose

Mammalian expression of mouse KIF1Balpha 387-1150

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1Balpha (Kif1b Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 386 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBABE puro human RXR alpha

Plasmid

#11441

Depositor

Ronald Kahn

Insert

RXR alpha (RXRA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
Myr-(iSH2-p85)-p110alpha-Myc

Plasmid

#1410

Depositor

Lewis Cantley

Insert

PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

SRalpha promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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FKBP-alpha(740-977)-GFP

Plasmid

#100731

Purpose

To express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

AP2A1 (Ap2a1 Mouse)

Use

Tags

FKBP and GFP

Expression

Mammalian

Mutation

Long isoform

Promoter

CMV

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pPICZ-pre-pro-alphaf(I)-E2-Crimson

Plasmid

#117660

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Benjamin Glick, Pau Ferrer

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf(I)-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe hygro human RXR alpha

Plasmid

#11440

Depositor

Ronald Kahn

Insert

RXR alpha (RXRA Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only
pDGB3 alpha2 P35S:dCas9:VPR:Tnos (GB1826)

Plasmid

#160623

Purpose

TU for the constitutive expression of dCas9 fused to VPR (VP64-p65-Rta) activation domains

Depositor

Diego Orzaez

Article

Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

Insert

P35S:dCas9:VPR-Tnos

Use

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBSSK p85 alpha (delta iSH2)

Plasmid

#13432

Depositor

Ronald Kahn

Article

Taniguchi et al Proc Natl Acad Sci U S A. 2006 Aug 8. 10

Insert

p85 alpha (delta iSH2) (PIK3R1 Human)

Use

Tags

flag

Expression

Bacterial

Mutation

Dominant negative p85 alpha. The p110 alpha bindi…

Promoter

Available since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pPICZ-pre-pro-alphaf-E2-Crimson

Plasmid

#117658

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Benjamin Glick, Pau Ferrer

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf-E2-Crimson

Use

Tags

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q

Plasmid

#172106

Purpose

Mammalian expression of Gαq fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq (GNAQ Human)

Use

Tags

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMKO.1 GFP B55 alpha shRNA 3-1

Plasmid

#10679

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha shRNA (PPP2R2A Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
Reduced Expression Alpha-5-Integrin-GFP

Plasmid

#30450

Depositor

Rick Horwitz

Insert

alpha 5 integrin (ITGA5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

91-544 of enhancer region of CMV promoter removed…

Promoter

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

Plasmid

#160608

Purpose

TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

Insert

P35s:dCas9:SRDX:tNOS

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

P35S

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-EF1alpha-VP64-dCas9-p300_Blast

Plasmid

#192654

Purpose

3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

VP64-dCas9-p300

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A; H840A in Cas9

Promoter

EF1a

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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VBS mutant of alpha catenin sensor

Plasmid

#71710

Purpose

This form of the alpha catenin conformation sensor lacks the vinculin binding site and does not bind vinculin.

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

alpha-catenin ΔVBS FRET Sensor

Use

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Deleted amino acid residues 316-405 in α -catenin…

Promoter

CMV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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