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Plasmid#83920PurposeCo-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.DepositorInsertsUseTagsIvy protein has C-terminal hisx6 tagExpressionBacterialMutationIvy leader sequence deleted and replaced.PromoterT7 and t7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only
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pRS406-CMV-lacI-3FLAG
Plasmid#83410PurposeExpression of lacI in yeastDepositorInsertlacI repressor
UseTags3xFLAG and NLSExpressionYeastMutationPromoterCMVAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV10-3xFLAG-prs-MCP1
Plasmid#184341PurposeExpress yeast MCP1 in Expi293F cellsDepositorInsertYeast MCP1 (MCP1 Budding Yeast)
UseTags3xFLAG-PreScission cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS303 Gal TDP-43 YFP
Plasmid#27468DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationD23A mutation in TDP-43 sequencePromoterAvailable sinceFeb. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal M337V YFP
Plasmid#27449DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationMethionine 337 mutated to Valine (M337V)PromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS6 AA 1035-1284
Plasmid#196904PurposeExpresses His-tagged Drosophila IntS6 amino acids 1035-1284DepositorInsertIntegrator subunit 6 (IntS6 Fly)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN1-C911
Plasmid#125784Purposea seed-matched control for pRSITEP-puro-shWRN1DepositorInsertshWRN1-C911 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mEos3.1 (mEosFP3.1-A69T)
Plasmid#99221PurposeBacterial expression of the primed conversion capable protein pr-mEos3.1 (mEos3.1-A69T).DepositorInsertpr-mEos3.1
UseTagsExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS11 AA 300-597
Plasmid#199329PurposeExpresses His-tagged Drosophila IntS11 amino acids 300-597DepositorInsertIntegrator subunit 11 (IntS11 Fly)
UseTags6xHisExpressionBacterialMutationamino acids 300-597 onlyPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
PRS415-Gal1pr-Mag1-sctetR
Plasmid#44752DepositorInsertMAG1-sctetR
UseTagsExpressionYeastMutationPromoterGal1prAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN2-C911
Plasmid#125786Purposea seed-matched control for pRSITEP-puro-shWRN2DepositorInsertshWRN2 (WRN Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Gal4-dCas9-VP64
Plasmid#71128PurposeThis plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.DepositorInsertGal4-dCas9-VP64
UseTagsNLS n-terminal, N terminal Gal4 Activator domain,…ExpressionYeastMutationPromoterTef1Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS41K-mScarlet-I-mNeonGreen (pKBJ010)
Plasmid#173461PurposeC-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timerDepositorInsertmScarlet-I-mNeonGreen
UseTagsExpressionYeastMutationPromoterno promoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PRS303-Gal1pr-Mag1-sctetR
Plasmid#44753DepositorInsertMAG1-sctetR
UseTagsExpressionYeastMutationPromoterGal1prAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc
Plasmid#91932PurposeBacterial expression of maize SUMO1a and SCE1bDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1b-Myc
UseTags6His and MycExpressionBacterialMutationTruncation of ZmSUMO1a after the diGlycine motifPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMW37: pRS426-R2Bm RNA
Plasmid#209291PurposeContains the R2Bm non-LTR retrotransposon 5'UTR and 3'UTR sequencesDepositorInsertR2Bm 5'UTR and 3'UTR
UseTagsExpressionBacterial and YeastMutationPromoterGAL1 GAPDHAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal G294A YFP
Plasmid#27448DepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionYeastMutationGlycine 294 mutated to Alanine (G294A)PromoterAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc
Plasmid#91934PurposeBacterial expression of maize SUMO1a and SCE1dDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1d-Myc
UseTags6His and MycExpressionBacterialMutationTruncation of ZmSUMO1a after the diGlycine motifPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
UseTagsExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETC-RdgBbeta-splice variant 1
Plasmid#58276Purposebacterial expression of human RdgBbeta splice variant 1DepositorInsertRdgBbeta (PITPNC1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only