We narrowed to 8,882 results for: plat

1. pRS263

   Plasmid

   #122379

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD364

   Plasmid

   #91831

   Purpose

   Lox511I-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Lox511I-SEC-Lox511I
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDD382

   Plasmid

   #91830

   Purpose

   Lox2272-flanked SEC donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Lox2272-SEC-Lox2272
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJAT21

   Plasmid

   #204309

   Purpose

   For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-GFPn
   

   Use

   CRISPR

   Promoter

   ie1

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAT25

   Plasmid

   #204310

   Purpose

   For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   ie1-GFPc
   

   Use

   CRISPR

   Promoter

   ie1

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMaCTag-07

   Plasmid

   #124786

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (without selection) with mNeonGreen

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mNeonGreen
   

   Use

   Pcr template

   Tags

   mNeonGreen

   Promoter

   None

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. B2395_CIBN-BTB-IDR-FRB

   Plasmid

   #215107

   Purpose

   Expresses CIBN-BTB-mCherry-FUS(IDR)-FRB in mammalian cells

   Depositor

   Chandra Tucker

   Article

   Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

   Insert

   CIBN-BTB-FUS(IDR)-FRB
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDB4282

   Plasmid

   #98701

   Purpose

   A plasmid containing the 3' portion of the ura4 marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-ura4 system

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   gRNA PCR template
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJAT56

   Plasmid

   #204300

   Purpose

   Express phiC31 under control of D. melanoagaster vasa promoter, with Vasa 3’UTR.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   3XP3-EGFP
   

   Use

   CRISPR

   Promoter

   3XP3

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJUMP18-Histag_C

    Plasmid

    #127035

    Purpose

    Basic Part C- C-terminus; polyhistidine tag

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part His6_C
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBSV2_Psyn-sgRNA500

    Plasmid

    #149613

    Purpose

    gRNA expression in B. burgdorferi, parental vector

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS267

    Plasmid

    #122380

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MXS_E2-Crimson

    Plasmid

    #62410

    Purpose

    MXS_chaining vector with E2-Crimson

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    E2-Crimson
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJUMP44-2A(sfGFP)

    Plasmid

    #126988

    Purpose

    Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. IVT-MCP-MMLV-RT

    Plasmid

    #207457

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    MCP-RT
    

    Expression

    Bacterial

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. TOPO mTagBFP2

    Plasmid

    #68413

    Purpose

    TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    mTagBFP2
    

    Use

    Gmap

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJUMP18-tetR_O

    Plasmid

    #127021

    Purpose

    Basic Part O- ORF; TetR; transcription factor

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Insert

    Part TetR_o
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKIKan_idCas9_Chr_center

    Plasmid

    #149637

    Purpose

    suicide vector for knock in into the B. burgdorferi chromosome

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PflaB, PpQE30

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB4283

    Plasmid

    #98702

    Purpose

    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    gRNA PCR template
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJAT27

    Plasmid

    #204308

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in flight muscles.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    MHC-GFPc
    

    Use

    CRISPR

    Promoter

    MHC

    Available Since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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